A multiplex real-time PCR method to detect and quantify mitochondrial DNA deletions in individual cells

2007 ◽  
Vol 370 (1) ◽  
pp. 127-129 ◽  
Author(s):  
Kim J. Krishnan ◽  
Andreas Bender ◽  
Robert W. Taylor ◽  
Douglass M. Turnbull
Keyword(s):  
Mitochondrial Dna ◽  
Real Time ◽  
Real Time Pcr ◽  
Dna Deletions ◽  
Pcr Method

scholarly journals Application of real-time PCR for DNA identification of ruminants in raw animal products

2018 ◽  
Vol 74 (1) ◽  
pp. 6061-2018
Author(s):  
ANNA WEINER ◽  
ILONA PAPROCKA ◽  
AGATA GOŁĘBIOWSKA ◽  
KRZYSZTOF KWIATEK
Keyword(s):  
Mitochondrial Dna ◽  
Real Time ◽  
Real Time Pcr ◽  
Animal Origin ◽  
Dna Identification ◽  
Animal Products ◽  
Modified Method ◽  
Pcr Method ◽  
Processed Products ◽  
By Products

According with current regulations it is possible export of processed animal proteins produced from tissues other animals than ruminants. In order to ensure appropriate control studies were undertaken to adopt of method real-time PCR used for analysis of processed products of animal origin. The material consisted of raw animal by-products of beef, pork and poultry. In the assays mitochondrial DNA was used. Modified method allows to detect the DNA of ruminants at the level of 0.025%. Based on obtained results of the validation studies were found that the real-time PCR method can be used in routine tests for identification of ruminant DNA in raw animal by-products..

A real-time PCR method for quantifying mixed cashmere and wool based on hair mitochondrial DNA

2014 ◽  
Vol 84 (15) ◽  
pp. 1612-1621 ◽  
Author(s):  
Minfeng Tang ◽  
Weiping Zhang ◽  
Hui Zhou ◽  
Jing Fei ◽  
Juan Yang ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Real Time ◽  
Real Time Pcr ◽  
Pcr Method

scholarly journals Triplex real-time PCR–an improved method to detect a wide spectrum of mitochondrial DNA deletions in single cells

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Karolina A. Rygiel ◽  
John P. Grady ◽  
Robert W. Taylor ◽  
Helen A. L. Tuppen ◽  
Doug M. Turnbull
Keyword(s):  
Mitochondrial Dna ◽  
Real Time ◽  
Real Time Pcr ◽  
Wide Spectrum ◽  
Single Cells ◽  
Improved Method ◽  
Dna Deletions

Development of a real-time PCR method for the simultaneous detection of soya and lupin mitochondrial DNA as markers for the presence of allergens in processed food

2011 ◽  
Vol 127 (2) ◽  
pp. 834-841 ◽  
Author(s):  
Antonio M. Gomez Galan ◽  
Marcel Brohée ◽  
Eugénia de Andrade Silva ◽  
Arjon J. van Hengel ◽  
Hubert Chassaigne
Keyword(s):  
Mitochondrial Dna ◽  
Real Time ◽  
Real Time Pcr ◽  
Simultaneous Detection ◽  
Processed Food ◽  
Pcr Method

Detection of Streptococcus pneumoniae in Sputum Samples by Real- Time PCR.

2020 ◽  
Vol 18 ◽  
Author(s):  
Pegah Shakib ◽  
Mohammad Reza Zolfaghari
Keyword(s):  
Streptococcus Pneumoniae ◽  
Real Time ◽  
Real Time Pcr ◽  
False Negative ◽  
Cross Sectional Study ◽  
Laboratory Culture ◽  
Cross Sectional ◽  
Negative Results ◽  
False Negative Results ◽  
Pcr Method

Background: Conventional laboratory culture-based methods for diagnosis of Streptococcus pneumoniae are time-consuming and yield false negative results. Molecular methods including real-time (RT)-PCR rapid methods and conventional PCR due to higher sensitivity and accuracy have been replaced instead traditional culture assay. The aim of the current study was to evaluate lytA gene for detection of Streptococcus pneumoniae in the cerebrospinal fluid of human patients with meningitis using real-time PCR assay. Material and Methods: In this cross-sectional study, a total of 30 clinical specimens were collected from patients in a period from September to December 2018. In order to evaluate the presence of lytA gene, conventional and real-time PCR methods were used without culture. Results: From 30 sputum samples five (16.66%) isolates were identified as S. pneumoniae by lytA PCR and sequencing. Discussion: In this research, an accurate and rapid real-time PCR method was used, which is based on lytA gene for diagnosis of bacteria so that it can be diagnosed. Based on the sequencing results, the sensitivity for detection of lytA gene was 100% (5/5).

scholarly journals Real-Time PCR Assay for Detection and Enumeration of Dekkera bruxellensis in Wine

2003 ◽  
Vol 69 (12) ◽  
pp. 7430-7434 ◽  
Author(s):  
Trevor G. Phister ◽  
David A. Mills
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Pcr Primers ◽  
Rrna Gene ◽  
Dekkera Bruxellensis ◽  
Pcr Assay ◽  
Specific Pcr ◽  
Spoilage Yeast ◽  
Pcr Method ◽  
26S Rrna

ABSTRACT Traditional methods to detect the spoilage yeast Dekkera bruxellensis from wine involve lengthy enrichments. To overcome this difficulty, we developed a quantitative real-time PCR method to directly detect and enumerate D. bruxellensis in wine. Specific PCR primers to D. bruxellensis were designed to the 26S rRNA gene, and nontarget yeast and bacteria common to the winery environment were not amplified. The assay was linear over a range of cell concentrations (6 log units) and could detect as little as 1 cell per ml in wine. The addition of large amounts of nontarget yeasts did not impact the efficiency of the assay. This method will be helpful to identify possible routes of D. bruxellensis infection in winery environments. Moreover, the time involved in performing the assay (3 h) should enable winemakers to more quickly make wine processing decisions in order to reduce the threat of spoilage by D. bruxellensis.

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