Modification of the Stewart biphasic colorimetric assay for stable and accurate quantitatitive determination of Pluronic and Tetronic block copolymers for application in biological systems

2007 ◽  
Vol 361 (2) ◽  
pp. 287-293 ◽  
Author(s):  
Othman Al-Hanbali ◽  
Nneka M. Onwuzo ◽  
Kenneth J. Rutt ◽  
Christopher M. Dadswell ◽  
S. Moein Moghimi ◽  
...  
Keyword(s):  
Block Copolymers ◽  
Colorimetric Assay ◽  
Biological Systems

Transmission Electron Microscopy of Protein Macromolecules

1971 ◽  
Vol 29 ◽  
pp. 424-425
Author(s):  
Henry S. Slayter
Keyword(s):  
Biological Systems ◽  
Metal Vapor ◽  
Resolving Power ◽  
Electron Microscopic ◽  
Chemical Methods ◽  
Molecular Weights ◽  
The Past ◽  
Physical Chemical ◽  
Transmission Electron

Electron microscopic methods have been applied increasingly during the past fifteen years, to problems in structural molecular biology. Used in conjunction with physical chemical methods and/or Fourier methods of analysis, they constitute powerful tools for determining sizes, shapes and modes of aggregation of biopolymers with molecular weights greater than 50, 000. However, the application of the e.m. to the determination of very fine structure approaching the limit of instrumental resolving power in biological systems has not been productive, due to various difficulties such as the destructive effects of dehydration, damage to the specimen by the electron beam, and lack of adequate and specific contrast. One of the most satisfactory methods for contrasting individual macromolecules involves the deposition of heavy metal vapor upon the specimen. We have investigated this process, and present here what we believe to be the more important considerations for optimizing it. Results of the application of these methods to several biological systems including muscle proteins, fibrinogen, ribosomes and chromatin will be discussed.

Development of a colorimetric protein phosphorylation assay for detecting cyanobacterial toxins

1995 ◽  
Vol 31 (5-6) ◽  
pp. 47-49 ◽  
Author(s):  
C. Ash ◽  
C. MacKintosh ◽  
R. MacKintosh ◽  
C. R. Fricker
Keyword(s):  
Protein Phosphorylation ◽  
Colorimetric Assay ◽  
Cyanobacterial Toxins ◽  
Sensitive Determination

A new colorimetric assay is described, based on inhibition of protein phosphotases, that enables the rapid, simple and sensitive determination of the concentration of toxins from cyanobacteria.

The Need for Using An Enzymatic Colorimetric Assay in Creatinine Determination of Peritoneal Dialysis Solutions

1990 ◽  
Vol 10 (1) ◽  
pp. 89-92 ◽  
Author(s):  
Liliane Larpent ◽  
Christian Verger
Keyword(s):  
Peritoneal Dialysis ◽  
Reliable Method ◽  
Colorimetric Assay ◽  
Colorimetric Method ◽  
Peritoneal Membrane ◽  
Creatinine Kinetics ◽  
Dialysis Solutions ◽  
Peritoneal Dialysis Solutions ◽  
Enzymatic Test

The fate of the peritoneal membrane on continuous ambulatory peritoneal dialysis (CAPD) is usually evaluated through the modification of its permeability to various solutes as glucose, creatinine, and urea. Therefore, the accuracy of the methods used for measurements of creatinine is of great importance. A particular problem does exist for creatinine determination as it may be influenced by the presence of glucose. We studied a new enzymatic colorimetric method for creatinine determination in peritoneal dialysis solutions which contain high dextrose concentrations. Creatinine was measured in plasma, urine, and dialysate from 18 patients on CAPD and in pure dextrose solutions, with an enzymatic test (Boehringer Mannheim) and with Jaffe's reaction on two different analyzers: Astra (Beckman) and Eris (Merck). Creatinine results were similar with both assays (Jaffe's reaction and enzymatic test) when measured in blood and urine. However the Jaffe's reaction gave higher creatinine results than the enzymatic test (p < 0.001), when assays were performed in peritoneal dialysis solutions and in pure glucose solutions. In addition, it appeared that other components of dialysis solutions, mainly calcium chloride, influenced unpredictably the results of creatinine with the Jaffe's reaction. We conclude that specific enzymatic test is a more accurate and reliable method to evaluate creatinine kinetics through the peritoneal membrane when determined in CAPD solutions.

Fluorescence chemosensors for hydrogen sulfide detection in biological systems

The Analyst ◽  
2015 ◽  
Vol 140 (6) ◽  
pp. 1772-1786 ◽  
Author(s):  
Zhi Guo ◽  
Guiqiu Chen ◽  
Guangming Zeng ◽  
Zhongwu Li ◽  
Anwei Chen ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Biological Systems ◽  
Sulfate Reducing Bacteria ◽  
Fluorescence Sensing ◽  
Sulfate Reducing ◽  
Potential Applications ◽  
Reducing Bacteria

The development of H2S fluorescence-sensing strategies and their potential applications in the determination of sulfate-reducing bacteria activity.

A novel colorimetric assay for the determination of lysophosphatidic acid in plasma using an enzymatic cycling method

2003 ◽  
Vol 333 (1) ◽  
pp. 59-67 ◽  
Author(s):  
Tatsuya Kishimoto ◽  
Takeshi Matsuoka ◽  
Shigeyuki Imamura ◽  
Koji Mizuno
Keyword(s):  
Lysophosphatidic Acid ◽  
Colorimetric Assay ◽  
Enzymatic Cycling
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