Kinetic analysis of enzymatic hydrolysis of crystalline cellulose by cellobiohydrolase using an amperometric biosensor

2006 ◽  
Vol 357 (2) ◽  
pp. 257-261 ◽  
Author(s):  
Hirosuke Tatsumi ◽  
Hajime Katano ◽  
Tokuji Ikeda
Keyword(s):  
Enzymatic Hydrolysis ◽  
Kinetic Analysis ◽  
Amperometric Biosensor ◽  
Crystalline Cellulose ◽  
Hydrolysis Of

scholarly journals Effect of Subsequent Dilute Acid and Enzymatic Hydrolysis on Reducing Sugar Production from Sugarcane Bagasse and Spent Citronella Biomass

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Robinson Timung ◽  
Narendra Naik Deshavath ◽  
Vaibhav V. Goud ◽  
Venkata V. Dasu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
Crystallinity Index ◽  
Sem Analysis ◽  
Reducing Sugar ◽  
Dilute Acid Pretreatment ◽  
Crystalline Cellulose ◽  
Dilute Acid ◽  
Acid Pretreatment ◽  
Hydrolysis Of

This work was aimed at investigating the effect of process parameters on dilute acid pretreatment and enzymatic hydrolysis of spent citronella biomass (after citronella oil extraction) and sugarcane bagasse on total reducing sugar (TRS) yield. In acid pretreatment, the parameters studied were acid concentration, temperature, and time. At the optimized condition (0.1 M H2SO4, 120°C, and 120 min), maximum TRS obtained was 452.27 mg·g−1and 487.50 mg·g−1for bagasse and citronella, respectively. Enzymatic hydrolysis of the pretreated biomass usingTrichoderma reesei26291 showed maximum TRS yield of 226.99 mg·g−1for citronella and 282.85 mg·g−1for bagasse at 10 FPU, 50°C, and 48 hr. The maximum crystallinity index (CI) of bagasse and citronella after acid pretreatment obtained from X-ray diffraction analysis was 64.41% and 56.18%, respectively. Decreased CI after enzymatic hydrolysis process to 37.28% and 34.16% for bagasse and citronella, respectively, revealed effective conversion of crystalline cellulose to glucose. SEM analysis of the untreated and treated biomass revealed significant hydrolysis of holocellulose and disruption of lignin.

Multifunctional cellulolytic auxiliary activity protein HcAA10-2 from Hahella chejuensis enhances enzymatic hydrolysis of crystalline cellulose

2014 ◽  
Vol 99 (7) ◽  
pp. 3041-3055 ◽  
Author(s):  
Sunil S. Ghatge ◽  
Amar A. Telke ◽  
Tatoba R. Waghmode ◽  
Yuno Lee ◽  
Keun-Woo Lee ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Crystalline Cellulose ◽  
Hahella Chejuensis ◽  
Hydrolysis Of

Enzymatic hydrolysis of fish protein by-products: Kinetic analysis

2008 ◽  
Vol 136 ◽  
pp. S378-S379
Author(s):  
S. Almonacid ◽  
C. Castillo ◽  
M. Pinto ◽  
A. Abakarov ◽  
R. Simpson
Keyword(s):  
Enzymatic Hydrolysis ◽  
Kinetic Analysis ◽  
Fish Protein ◽  
Hydrolysis Of ◽  
By Products

Kinetic analysis of an enzymatic hydrolysis of p-nitrophenyl acetate with carboxylesterase by pressure-assisted capillary electrophoresis/dynamic frontal analysis

2020 ◽  
Vol 12 (48) ◽  
pp. 5846-5851
Author(s):  
Masanori Mine ◽  
Naoya Matsumoto ◽  
Hitoshi Mizuguchi ◽  
Toshio Takayanagi
Keyword(s):  
Capillary Electrophoresis ◽  
Enzymatic Hydrolysis ◽  
Kinetic Analysis ◽  
Frontal Analysis ◽  
Hydrolysis Of

An enzymatic hydrolysis of p-nitrophenyl acetate with carboxylesterase was analyzed by capillary electrophoresis/dynamic frontal analysis (CE/DFA).

scholarly journals Surface Pitting on Nanofibrous Matrix as an Ultra- Sensitive Indicator for Enzymatic Hydrolysis of Crystalline Cellulose

2021 ◽  
Author(s):  
mikiko tsudome ◽  
Mikako Tachioka ◽  
Miwako Tsuda ◽  
Yoshihiro Takaki ◽  
Shigeru Deguchi
Keyword(s):  
Enzymatic Hydrolysis ◽  
Enzymatic Degradation ◽  
Synthetic Polymers ◽  
Water Soluble ◽  
Crystalline Cellulose ◽  
Optical Profilometry ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Reaction Proceeds ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

<p>Assaying enzymatic degradation of the water-insoluble substrate such as cellulose and synthetic polymers has remained technically challenging, primarily because only the surface of the substrate is accessible to the enzymes and the reaction proceeds very slowly compared with those of water-soluble substrates. Here we show an ultra-sensitive and semi-quantitative assay for enzymatic hydrolysis of cellulose. By combining nanofibrous matrices with piezo-driven inkjet printing and optical profilometry, enzymatic hydrolysis of less than 1 nanogram of crystalline cellulose was successfully quantified. Unprecedented genetic diversity of cellulase was revealed when the same principle was applied for elucidating microbial degradation of cellulose in the deep sea. This work demonstrates that truly interdisciplinary efforts, encompassing diverse disciplines from nanotechnology to microbiology, are crucial to address scientific and technological problems towards sustainability.<br></p>

Kinetic modeling of rapid enzymatic hydrolysis of crystalline cellulose after pretreatment by NMMO

2011 ◽  
Vol 39 (3) ◽  
pp. 429-438 ◽  
Author(s):  
Mahdi Khodaverdi ◽  
Azam Jeihanipour ◽  
Keikhosro Karimi ◽  
Mohammad J. Taherzadeh
Keyword(s):  
Enzymatic Hydrolysis ◽  
Kinetic Modeling ◽  
Crystalline Cellulose ◽  
Hydrolysis Of

scholarly journals A pyranose dehydrogenase-based biosensor for kinetic analysis of enzymatic hydrolysis of cellulose by cellulases

2014 ◽  
Vol 58-59 ◽  
pp. 68-74 ◽  
Author(s):  
Nicolaj Cruys-Bagger ◽  
Silke Flindt Badino ◽  
Radina Tokin ◽  
Mark Gontsarik ◽  
Samin Fathalinejad ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Kinetic Analysis ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Hydrolysis Of Cellulose ◽  
Pyranose Dehydrogenase ◽  
Hydrolysis Of
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