Automated high-throughput process for site-directed mutagenesis, production, purification, and kinetic characterization of enzymes

Raphaël Carapito; Benoit Gallet; André Zapun; Thierry Vernet

doi:10.1016/j.ab.2006.04.047

Automated high-throughput process for site-directed mutagenesis, production, purification, and kinetic characterization of enzymes

Analytical Biochemistry ◽

10.1016/j.ab.2006.04.047 ◽

2006 ◽

Vol 355 (1) ◽

pp. 110-116 ◽

Cited By ~ 11

Author(s):

Raphaël Carapito ◽

Benoit Gallet ◽

André Zapun ◽

Thierry Vernet

Keyword(s):

High Throughput ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Kinetic Characterization

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Kinetic characterization of the recombinant ribonuclease from Bacillus amyloliquefaciens (barnase) and investigation of key residues in catalysis by site-directed mutagenesis

Biochemistry ◽

10.1021/bi00435a033 ◽

1989 ◽

Vol 28 (9) ◽

pp. 3843-3850 ◽

Cited By ~ 106

Author(s):

Danuta E. Mossakowska ◽

Kerstin Nyberg ◽

Alan R. Fersht

Keyword(s):

Bacillus Amyloliquefaciens ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Kinetic Characterization ◽

Key Residues

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Characterization of the phosphotransferase domain of casein kinase II by site-directed mutagenesis and expression in Escherichia coli.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)35921-0 ◽

1992 ◽

Vol 267 (33) ◽

pp. 23894-23902

Author(s):

R Jakobi ◽

J.A. Traugh

Keyword(s):

Escherichia Coli ◽

Casein Kinase Ii ◽

Casein Kinase ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Expression In Escherichia Coli

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Characterization of regions of fibronectin besides the arginine-glycine-aspartic acid sequence required for adhesive function of the cell-binding domain using site-directed mutagenesis

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)98498-x ◽

1991 ◽

Vol 266 (24) ◽

pp. 15938-15943 ◽

Cited By ~ 3

Author(s):

S. Aota ◽

T. Nagai ◽

K.M. Yamada

Keyword(s):

Aspartic Acid ◽

Binding Domain ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Cell Binding ◽

Arginine Glycine Aspartic Acid ◽

Cell Binding Domain

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Characterization of cathepsin B specificity by site-directed mutagenesis. Importance of Glu245 in the S2-P2 specificity for arginine and its role in transition state stabilization.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)54140-5 ◽

1993 ◽

Vol 268 (1) ◽

pp. 235-240

Author(s):

S. Hasnain ◽

T. Hirama ◽

C.P. Huber ◽

P. Mason ◽

J.S. Mort

Keyword(s):

Transition State ◽

Cathepsin B ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Transition State Stabilization

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Site-directed mutagenesis of conserved cysteine residues within the .beta. subunit of Escherichia coli nitrate reductase. Physiological, biochemical, and EPR characterization of the mutated enzymes

Biochemistry ◽

10.1021/bi00059a018 ◽

1993 ◽

Vol 32 (8) ◽

pp. 2013-2023 ◽

Cited By ~ 31

Author(s):

Valerie Augier ◽

Bruno Guigliarelli ◽

Marcel Asso ◽

Patrick Bertrand ◽

Chantal Frixon ◽

...

Keyword(s):

Escherichia Coli ◽

Nitrate Reductase ◽

Beta Subunit ◽

Site Directed Mutagenesis ◽

Cysteine Residues ◽

Directed Mutagenesis

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Characterization of inhibitor binding to human kinesin spindle protein by site-directed mutagenesis

Archives of Biochemistry and Biophysics ◽

10.1016/j.abb.2009.01.015 ◽

2009 ◽

Vol 484 (1) ◽

pp. 1-7 ◽

Cited By ~ 5

Author(s):

C. Gary Marshall ◽

Maricel Torrent ◽

Olusegun Williams ◽

Kelly A. Hamilton ◽

Carolyn A. Buser

Keyword(s):

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Inhibitor Binding ◽

Kinesin Spindle Protein

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Characterization of Loops of the Yeast Mitochondrial ADP/ATP Carrier Facing the Cytosol by Site-Directed Mutagenesis

Biochemical and Biophysical Research Communications ◽

10.1006/bbrc.2001.5498 ◽

2001 ◽

Vol 286 (5) ◽

pp. 936-942 ◽

Cited By ~ 16

Author(s):

Takashi Hatanaka ◽

Yoshitaka Kihira ◽

Yasuo Shinohara ◽

Eiji Majima ◽

Hiroshi Terada

Keyword(s):

Site Directed Mutagenesis ◽

Directed Mutagenesis

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Characterization of the Human Spuma Retrovirus Integrase by Site-directed Mutagenesis, by Complementation Analysis, and by Swapping the Zinc Finger Domain of HIV-1

Journal of Biological Chemistry ◽

10.1074/jbc.270.7.2957 ◽

1995 ◽

Vol 270 (7) ◽

pp. 2957-2966 ◽

Cited By ~ 35

Author(s):

Armin Pahl ◽

Rolf M. Flügel

Keyword(s):

Zinc Finger ◽

Site Directed Mutagenesis ◽

Complementation Analysis ◽

Directed Mutagenesis ◽

Zinc Finger Domain ◽

Hiv 1

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Characterization of an Autoreduction Pathway for the [Fe4S4]3+Cluster of MutantChromatium vinosumHigh-Potential Iron Proteins. Site-Directed Mutagenesis Studies To Probe the Role of Phenylalanine 66 in Defining the Stability of the [Fe4S4] Center Provide Evidence for Oxidative Degradation via a [Fe3S4] Cluster†

Biochemistry ◽

10.1021/bi961658l ◽

1996 ◽

Vol 35 (46) ◽

pp. 14544-14552 ◽

Cited By ~ 13

Author(s):

Shumin Bian ◽

C. F. Hemann ◽

Russ Hille ◽

J. A. Cowan

Keyword(s):

Oxidative Degradation ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Iron Proteins ◽

The Stability

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Characterization of the Ferrous Ion Binding Sites of Apple 1-Aminocyclopropane-1-carboxylate Oxidase by Site-Directed Mutagenesis

Biochemical and Biophysical Research Communications ◽

10.1006/bbrc.1996.1237 ◽

1996 ◽

Vol 225 (3) ◽

pp. 697-700 ◽

Cited By ~ 26

Author(s):

Jei-Fu Shaw ◽

Yun-Shan Chou ◽

Rey-Chang Chang ◽

Shang Fa Yang

Keyword(s):

Binding Sites ◽

Site Directed Mutagenesis ◽

Ferrous Ion ◽

Directed Mutagenesis ◽

Ion Binding ◽

Ion Binding Sites

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