A two-step procedure for extracting genomic DNA from dried blood spots on filter paper for polymerase chain reaction amplification

2006 ◽  
Vol 354 (1) ◽  
pp. 159-161 ◽  
Author(s):  
H. Zhou ◽  
J.G.H. Hickford ◽  
Q. Fang
Keyword(s):  
Polymerase Chain Reaction ◽  
Filter Paper ◽  
Genomic Dna ◽  
Polymerase Chain Reaction Amplification ◽  
Dried Blood Spots ◽  
Chain Reaction ◽  
Blood Spots ◽  
Step Procedure ◽  
Reaction Amplification ◽  
Polymerase Chain

Two Novel Nonradioactive Polymerase Chain Reaction-Based Assays of Dried Blood Spots, Genomic DNA, or Whole Cells for Fast, Reliable Detection of Z and S Mutations in the α1-Antitrypsin Gene

1992 ◽  
Vol 38 (10) ◽  
pp. 2100-2107 ◽  
Author(s):  
B S Andresen ◽  
I Knudsen ◽  
P K Jensen ◽  
K Rasmussen ◽  
N Gregersen
Keyword(s):  
Polymerase Chain Reaction ◽  
Genomic Dna ◽  
Dried Blood Spots ◽  
Chain Reaction ◽  
Tissue Samples ◽  
Whole Cells ◽  
Blood Spots ◽  
Allele Specific ◽  
Polymerase Chain ◽  
Alpha 1 Antitrypsin

Abstract Two new nonradioactive polymerase chain reaction (PCR)-based assays for the Z and S mutations in the alpha 1-antitrypsin gene are presented. The assays take advantage of PCR-mediated mutagenesis, creating new diagnostic restriction enzyme sites for unambiguous discrimination between test samples from individuals who are normal, heterozygous, or homozygous for the mutations. We show that the two assays can be performed with purified genomic DNA as well as with boiled blood spots. The new assays were validated by parallel testing with a technique in which PCR is combined with allele-specific oligonucleotide (ASO) probes. In all cases tested the results obtained by the different techniques were in accordance. The new assays can be used for prenatal diagnostics and can be performed directly with boiled tissue samples. Because the new assays are easy to perform and reliable, we conclude that they are well suited for routine diagnosis.

scholarly journals P-SELECTIN GLYCOPROTEIN LIGAND-1 GENE POLYMORPHISMS IN SUDANESE PREGNANT WOMEN WITH THROMBOSIS

2018 ◽  
Vol 11 (12) ◽  
pp. 226
Author(s):  
Abdelrahman A Elhassan ◽  
Fathelrahman Mahdi Hassan ◽  
Hanan B Eltahir
Keyword(s):  
Polymerase Chain Reaction ◽  
Pregnant Women ◽  
Gene Polymorphisms ◽  
Genomic Dna ◽  
Polymerase Chain Reaction Amplification ◽  
Healthy Controls ◽  
Chain Reaction ◽  
Increased Risk ◽  
Reaction Amplification ◽  
Polymerase Chain

Objectives: In this study we aimed to investigate whether P-selectin gene polymorphism is associated with thrombosis in Sudanese pregnant women in Khartoum state.Methods: After informed consent, 96 Sudanese pregnant women with thrombosis and 53 healthy pregnant women were recruited in the study. Genomic DNA was isolated from whole blood. Genotyping of PSGL-1 gene was performed by polymerase chain reaction amplification for exon 14 and electrophoresis.Results: The frequency of the B allele was found to be significantly higher in pregnant women with thrombosis (26%) compared to the controls (17.4%).Conclusion: The AB genotype was found to be higher in women with thrombosis 40.6% than in healthy controls 20% (p=0.02). Our results suggest that the PSGL-1 AB genotype is associated with an increased risk of thrombosis in Sudanese pregnant women.

Sign in / Sign up

Export Citation Format

Share Document