Rapid detection of Clostridium botulinum toxins A, B, E, and F in clinical samples, selected food matrices, and buffer using paramagnetic bead-based electrochemiluminescence detection

2006 ◽  
Vol 353 (2) ◽  
pp. 248-256 ◽  
Author(s):  
Victor R. Rivera ◽  
Frank J. Gamez ◽  
William K. Keener ◽  
Jill A. White ◽  
Mark A. Poli
Keyword(s):  
Rapid Detection ◽  
Clostridium Botulinum ◽  
Clinical Samples ◽  
Botulinum Toxins ◽  
Food Matrices ◽  
Paramagnetic Bead

scholarly journals Rapid Detection of Clostridium botulinum in Food Using Loop-Mediated Isothermal Amplification (LAMP)

2021 ◽  
Vol 18 (9) ◽  
pp. 4401
Author(s):  
Yufei Chen ◽  
Hao Li ◽  
Liu Yang ◽  
Lei Wang ◽  
Ruyi Sun ◽  
...  
Keyword(s):  
Rapid Detection ◽  
High Throughput Screening ◽  
Clostridium Botulinum ◽  
Lamp Assay ◽  
High Specificity ◽  
Isothermal Amplification ◽  
Clinical Samples ◽  
Loop Mediated Isothermal Amplification ◽  
Specificity And Sensitivity ◽  
Target Dna

Botulinum neurotoxins are considered as one of the most potent toxins and are produced by Clostridium botulinum. It is crucial to have a rapid and sensitive method to detect the bacterium Clostridium botulinum in food. In this study, a rapid detection assay of C. botulinum in food using loop-mediated isothermal amplification (LAMP) technology was developed. The optimal primers were identified among three sets of primers designed specifically based on the partial ntnh gene encoding nontoxic-nonhaemagglutinin (NTNH) for rapid detection of the target DNA in plasmids. The optimal temperature and reaction time of the LAMP assay were determined to be 64 °C and 60 min, respectively. The chemical kit could be assembled based on these optimized reaction conditions for quick, initial high-throughput screening of C. botulinum in food samples. The established LAMP assay showed high specificity and sensitivity in detecting the target DNA with a limit of 0.0001 pg/ul (i.e., ten times more sensitive than that of the PCR method) and an accuracy rate of 100%. This study demonstrated a potentially rapid, cost-effective, and easy-operating method to detect C. botulinum in food and clinical samples based on LAMP technology.

Survey of Clostridium botulinum toxins in Iranian traditional food products

2009 ◽  
Vol 19 (3) ◽  
pp. 247-250 ◽  
Author(s):  
Hedayat Hosseini ◽  
Hamid Reza Tavakoli ◽  
Mahzad Aghazadeh Meshgi ◽  
Ramin Khaksar ◽  
Marzieh Hosseini ◽  
...  
Keyword(s):  
Clostridium Botulinum ◽  
Food Products ◽  
Traditional Food ◽  
Botulinum Toxins

Preparation of specifically activatable endopeptidase derivatives of Clostridium botulinum toxins type A, B, and C and their applications

2005 ◽  
Vol 40 (1) ◽  
pp. 31-41 ◽  
Author(s):  
J. Mark Sutton ◽  
Jonathan Wayne ◽  
Anthony Scott-Tucker ◽  
Susan M. O’Brien ◽  
Philip M.H. Marks ◽  
...  
Keyword(s):  
Clostridium Botulinum ◽  
Type A ◽  
Botulinum Toxins ◽  
Derivatives Of ◽  
Botulinum Toxins Type A

scholarly journals Microcolony detection in thin layer culture as an alternative method for rapid detection of Mycobacterium tuberculosis in clinical samples

2007 ◽  
Vol 38 (3) ◽  
pp. 421-423 ◽  
Author(s):  
Pedro Eduardo Almeida da Silva ◽  
Fernanda Wiesel ◽  
Maria Marta Santos Boffo ◽  
Andréa von Groll ◽  
Ivo Gomes de Mattos ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Thin Layer ◽  
Rapid Detection ◽  
Clinical Samples ◽  
Alternative Method

Rapid detection of Escherichia coli O157:H7 spiked into food matrices

2007 ◽  
Vol 584 (1) ◽  
pp. 66-71 ◽  
Author(s):  
Lisa C. Shriver-Lake ◽  
Stephanie Turner ◽  
Chris R. Taitt
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Escherichia Coli O157 ◽  
Food Matrices

A new MSPQC system for rapid detection of pathogens in clinical samples

2006 ◽  
Vol 66 (1) ◽  
pp. 56-62 ◽  
Author(s):  
Fengjiao He ◽  
Xiaoqing Zhang ◽  
Jiandang Zhou ◽  
Zhenhua Liu
Keyword(s):  
Rapid Detection ◽  
Clinical Samples

Development of multiplex PCR assay for rapid detection Listeria monocytogenes in clinical samples

2021 ◽  
Vol 30 (4) ◽  
pp. 20-26
Author(s):  
Le Thanh Huong ◽  
Ha Thi Phuong Mai ◽  
Hoang Thi Thu Ha ◽  
Nguyen Dong Tu ◽  
Bui Tien Sy ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Multiplex Pcr ◽  
Rapid Detection ◽  
Pathogenic Bacteria ◽  
Vulnerable Groups ◽  
Clinical Samples ◽  
Specific Gene ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Specificity And Sensitivity

Listeria monocytogenes is widely present in the natural environment. This bacteria can cause infections in both humans and animals. In humans, the most vulnerable groups to be infected with L. monocytogenes are the elderly, people with an impaired immune system and chronically illness, pregnant women, and newborn babies. The aim of this study was to develop a multiplex PCR assay for the rapid detection of L. monocytogenes in mock clinical samples. A pair of primers were designed for detection of L. monocytogenes based on prs, a Listeria genus specific gene, and hly, a hemolysin gene. The specificity of the primers were tested by using different L. monocytogenes strains and other common pathogenic bacteria. The results showed that L. monocytogenes strains were positive in the detection and other tested strains were negative in mock (spiked) clinical samples. The sensitivity of multiplex PCR assay was 102 CFU/ml per reaction. The specificity and sensitivity of multiplex PCR technology for detecting L. monocytogenes in mock (spiked) clinical samples were high, and the assay could be completed within 1.5 hours. Therefore, this established multiplex PCR provides a rapid and reliable method and will be useful for the detection of L. monocytogenes in mock clinical samples.

scholarly journals Survey and Rapid Detection of Bordetella pertussis in Clinical Samples Targeting the BP485 in China

2015 ◽  
Vol 3 ◽  
Author(s):  
Wei Liu ◽  
Yinghua Xu ◽  
Derong Dong ◽  
Huan Li ◽  
Xiangna Zhao ◽  
...  
Keyword(s):  
Bordetella Pertussis ◽  
Rapid Detection ◽  
Clinical Samples
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