Determination of denaturated proteins and biotoxins by on-line size-exclusion chromatography–digestion–liquid chromatography–electrospray mass spectrometry

2005 ◽  
Vol 346 (1) ◽  
pp. 150-157 ◽  
Author(s):  
Jeroen Carol ◽  
Maarten C.J.K. Gorseling ◽  
Camiel F. de Jong ◽  
Henk Lingeman ◽  
Charles E. Kientz ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Size Exclusion Chromatography ◽  
Electrospray Mass Spectrometry ◽  
Size Exclusion ◽  
Exclusion Chromatography ◽  
On Line

scholarly journals Coupling of Size-Exclusion Chromatography to Liquid Chromatography/Mass Spectrometry for Determination of Trace Levels of Thifensulfuron-Methyl and Tribenuron-Methyl in Cottonseed and Cotton Gin Trash

2000 ◽  
Vol 83 (3) ◽  
pp. 651-659 ◽  
Author(s):  
James J Stry ◽  
Jennifer S Amoo ◽  
Steve W George ◽  
Teresa Hamilton-Johnson ◽  
Ellen Stetser
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Size Exclusion Chromatography ◽  
Reversed Phase ◽  
Size Exclusion ◽  
Liquid Chromatography Mass Spectrometry ◽  
Exclusion Chromatography ◽  
Tribenuron Methyl ◽  
Cotton Gin

Abstract Size-exclusion chromatography (SEC) was coupled to reversed-phase liquid chromatography/mass spectrometry for the determination of thifensulfuron-methyl and tribenuron-methyl in cottonseed and cotton gin trash. The limit of quantitation was 20 parts per billion (ppb), and the limit of detection was 6 ppb. The analytes were extracted by homogenization in a buffer solution. The extracts underwent a solvent exchange into methanol and were injected onto an SEC column. As the analytes eluted from the SEC column, the eluate was diverted onto a reversed-phase column for additional separation of the analytes and their detection via mass spectrometry. This method is unique because the samples are not cleaned up before analysis, the analytes are injected in methanol, and the entire analysis is completed in 30 min. Average recoveries and standard deviations for thifensulfuronmethyl and tribenuron-methyl in cotton gin trash were 91 ± 6% and 88 ± 5%, respectively. Average recoveries and standard deviations for thifensulfuron-methyl and tribenuron-methyl in cottonseed were 91 ± 11% and 99 ± 12%, respectively. This is an effective method for the detection and determination of thifensulfuron-methyl and tribenuronmethyl in cotton.

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