Characterization of GSTM3 polymorphism by real-time polymerase chain reaction with LightCycler

2004 ◽  
Vol 330 (1) ◽  
pp. 175-177
Author(s):  
Paola Mozzoni ◽  
Giuseppe De Palma ◽  
Eleonora Scotti ◽  
Marzia Capelletti ◽  
Antonio Mutti
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Chain Reaction ◽  
Polymerase Chain

Characterization of high-capacity adenovirus production by the quantitative real-time polymerase chain reaction: a comparative study of different titration methods

2008 ◽  
Vol 10 (10) ◽  
pp. 1092-1101 ◽  
Author(s):  
Julien Crettaz ◽  
Cristina Olague ◽  
Africa Vales ◽  
Igor Aurrekoetxea ◽  
Pedro Berraondo ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Comparative Study ◽  
Real Time ◽  
High Capacity ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Molecular characterization of field isolates of Avian Infectious Laryngeotracheitis Virus from different farms in Iraq

2017 ◽  
Vol 40 (2) ◽  
pp. 31-35
Author(s):  
Aida Bara Allawe
Keyword(s):  
Polymerase Chain Reaction ◽  
Positive Result ◽  
Real Time ◽  
Virus Detection ◽  
Chain Reaction ◽  
Glycoprotein G ◽  
Polymerase Chain ◽  
Labeled Probe ◽  
First Time

     This study was conducted to detect virulent isolates of avian infectious laryngeotracheitis virus in Iraq by Real Time-Polymerase Chain Reaction with the amplification of glycoprotein G gene which is responsible for virulence of the virus. Seventy samples (larynx and trachea) were collected from different farms in Iraq to investigate presence of avian infectious laryngeotracheitis virus (detection of virulent isolates from other vaccine strains). Five samples out of seventy samples were virulent isolates (positive result) by using Real Time-Polymerase Chain Reaction utilizing flurescein amidite labeled probe specific for detection of isolates that have G gene (by amplification of G gene) for the first time in Iraq. These virulent isolates were negative by using Real Time-Polymerase Chain Reaction utilizing Quasar-labeled probe specific for the detection of attenuated isolates that lack G gene and targeted a region within glycoprotein J downstream from the sequence of glycoprotein G.

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