Determination of granulocyte colony stimulating factor and its antibody in human serum samples using the BIAcore 3000 biosensor

2004 ◽  
Vol 324 (2) ◽  
pp. 304-306 ◽  
Author(s):  
Ateeq Ahmad ◽  
Qing Zong ◽  
Marie Rock ◽  
Matthew McLean ◽  
Alan Breau
Keyword(s):  
Human Serum ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Serum Samples ◽  
Human Serum Samples ◽  
Stimulating Factor

scholarly journals Characterization of recombinant-derived granulocyte-colony stimulating factor (G-CSF)

1988 ◽  
Vol 256 (1) ◽  
pp. 213-218 ◽  
Author(s):  
P Wingfield ◽  
R Benedict ◽  
G Turcatti ◽  
B Allet ◽  
J J Mermod ◽  
...  
Keyword(s):  
Coli Strain ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Wild Type ◽  
Disulphide Bonds ◽  
Disulphide Bond Formation ◽  
Stimulating Factor ◽  
Factor G

Human granulocyte colony-stimulating factor (G-CSF), and a mutant having a Ser for Cys substitution at residue 18 were produced in Escherichia coli strain W3110. About 60 mg of pure protein was obtained from 50 g of wet cells with a recovery of about 20%. The proteins were characterized physically and chemically, including determination of disulphide bonds, which were found to exist between residues 37-43 and 65-75. Cys-18 is not involved in disulphide bond formation and was substituted by Ser with no effects on gross protein conformation or biological activity. Both the wild-type and the mutant recombinant-derived proteins, although not glycosylated, possess colony-stimulating activities. In a bioassay using the murine myelomonocytic leukaemic cell line WEH1 3B D+, activities were obtained which were similar to those of natural G-CSF and of a glycosylated recombinant-derived human G-CSF produced in monkey cells.

Simultaneous determination of ascorbic and uric acids and dopamine in human serum samples using three-way calibration with data from square wave voltammetry

2016 ◽  
Vol 129 ◽  
pp. 205-212 ◽  
Author(s):  
Adrian Marcelo Granero ◽  
Gastón Darío Pierini ◽  
Sebastián Noel Robledo ◽  
María Susana Di Nezio ◽  
Héctor Fernández ◽  
...  
Keyword(s):  
Human Serum ◽  
Simultaneous Determination ◽  
Square Wave Voltammetry ◽  
Serum Samples ◽  
Square Wave ◽  
Human Serum Samples ◽  
Wave Voltammetry

scholarly journals Preclinical immunogenicity testing using anti-drug antibody analysis of GX-G3, Fc-fused recombinant human granulocyte colony-stimulating factor, in rat and monkey models

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yun Jung Kim ◽  
Eun Mi Koh ◽  
Chi Hun Song ◽  
Mi Sun Byun ◽  
Yu Ri Choi ◽  
...  
Keyword(s):  
Recovery Period ◽  
White Blood Cells ◽  
Serum Levels ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Serum Samples ◽  
Toxicological Studies ◽  
Validation Parameters ◽  
Immunogenicity Assessment ◽  
Stimulating Factor

AbstractHuman granulocyte colony-stimulating factor (G-CSF, this study used Fc-fused recombinant G-CSF; GX-G3) is an important glycoprotein that stimulates the proliferation of granulocytes and white blood cells. Thus, G-CSF treatment has been considered as a crucial regimen to accelerate recovery from chemotherapy-induced neutropenia in cancer patients suffering from non-myeloid malignancy or acute myeloid leukemia. Despite the therapeutic advantages of G-CSF treatment, an assessment of its immunogenicity must be performed to determine whether the production of anti-G-CSF antibodies causes immune-related disorders. We optimized and validated analytical tools by adopting validation parameters for immunogenicity assessment. Using these validated tools, we analyzed serum samples from rats and monkeys injected subcutaneously with GX-G3 (1, 3 or 10 mg/kg once a week for 4 weeks followed by a 4-week recovery period) to determine immunogenicity response and toxicokinetic parameters with serum concentration of GX-G3. Several rats and monkeys were determined to be positive for anti-GX-G3 antibodies. Moreover, the immunogenicity response of GX-G3 was lower in monkeys than in rats, which was relevant to show less inhibition of toxicokinetic profiles in monkeys, at least 1 mg/kg administrated group, compared to rats. These results suggested the establishment and validation for analyzing anti-GX-G3 antibodies and measurement of serum levels of GX-G3 and anti-GX-G3 antibodies, which was related with toxicokinetic profiles. Taken together, this study provides immunogenicity assessment which is closely implicated with toxicokinetic study of GX-G3 in 4-week repeated administrated toxicological studies.

Determination of ciprofloxacin and enoxacin in human serum samples by micellar liquid chromatography

2004 ◽  
Vol 516 (1-2) ◽  
pp. 135-140 ◽  
Author(s):  
José Luis Vı́lchez ◽  
Lilia Araujo ◽  
Avismelsi Prieto ◽  
Alberto Navalón
Keyword(s):  
Liquid Chromatography ◽  
Human Serum ◽  
Micellar Liquid Chromatography ◽  
Serum Samples ◽  
Human Serum Samples

scholarly journals Determination of Direct Oral Anticoagulants from Human Serum Samples

2013 ◽  
Vol 40 (01) ◽  
pp. 129-134
Author(s):  
Sandra Kraemer ◽  
Shanshan Du ◽  
Christina Giese ◽  
Astrid Schulze ◽  
Roland Kraemer ◽  
...  
Keyword(s):  
Human Serum ◽  
Oral Anticoagulants ◽  
Direct Oral Anticoagulants ◽  
Serum Samples ◽  
Human Serum Samples
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