scholarly journals Morphological structure of shell and expression patterns of five matrix protein genes during the shell regeneration process in Hyriopsis cumingii

2018 ◽  
Vol 3 (6) ◽  
pp. 225-231
Author(s):  
Xiaojun Liu ◽  
Can Jin ◽  
Haoran Li ◽  
Zhiyi Bai ◽  
Jiale Li
Keyword(s):  
Matrix Protein ◽  
Expression Patterns ◽  
Morphological Structure ◽  
Regeneration Process ◽  
Hyriopsis Cumingii ◽  
Shell Regeneration

scholarly journals Hic74, a novel alanine and glycine rich matrix protein related to nacreous layer formation in the mollusc Hyriopsis cumingii

2017 ◽  
Vol 2 (3) ◽  
pp. 119-123 ◽  
Author(s):  
Xiaojun Liu ◽  
Can Jin ◽  
Leiming Wu ◽  
Shaojian Dong ◽  
Shimei Zeng ◽  
...  
Keyword(s):  
Matrix Protein ◽  
Nacreous Layer ◽  
Layer Formation ◽  
Hyriopsis Cumingii

scholarly journals Transcription Factor Erg Variants and Functional Diversification of Chondrocytes during Limb Long Bone Development

2000 ◽  
Vol 150 (1) ◽  
pp. 27-40 ◽  
Author(s):  
Masahiro Iwamoto ◽  
Yoshinobu Higuchi ◽  
Eiki Koyama ◽  
Motomi Enomoto-Iwamoto ◽  
Kojiro Kurisu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Limb Development ◽  
Matrix Protein ◽  
Molecular Mechanisms ◽  
Articular Chondrocytes ◽  
Bone Cells ◽  
Expression Patterns ◽  
Extracellular Matrix Protein ◽  
Long Bone ◽  
Chondrocyte Maturation

During limb development, chondrocytes located at the epiphyseal tip of long bone models give rise to articular tissue, whereas the more numerous chondrocytes in the shaft undergo maturation, hypertrophy, and mineralization and are replaced by bone cells. It is not understood how chondrocytes follow these alternative pathways to distinct fates and functions. In this study we describe the cloning of C-1-1, a novel variant of the ets transcription factor ch-ERG. C-1-1 lacks a short 27–amino acid segment located ∼80 amino acids upstream of the ets DNA binding domain. We found that in chick embryo long bone anlagen, C-1-1 expression characterizes developing articular chondrocytes, whereas ch-ERG expression is particularly prominent in prehypertrophic chondrocytes in the growth plate. To analyze the function of C-1-1 and ch-ERG, viral vectors were used to constitutively express each factor in developing chick leg buds and cultured chondrocytes. We found that virally driven expression of C-1-1 maintained chondrocytes in a stable and immature phenotype, blocked their maturation into hypertrophic cells, and prevented the replacement of cartilage with bone. It also induced synthesis of tenascin-C, an extracellular matrix protein that is a unique product of developing articular chondrocytes. In contrast, virally driven expression of ch-ERG significantly stimulated chondrocyte maturation in culture, as indicated by increases in alkaline phosphatase activity and deposition of a mineralized matrix; however, it had modest effects in vivo. The data show that C-1-1 and ch-ERG have diverse biological properties and distinct expression patterns during skeletogenesis, and are part of molecular mechanisms by which limb chondrocytes follow alternative developmental pathways. C-1-1 is the first transcription factor identified to date that appears to be instrumental in the genesis and function of epiphyseal articular chondrocytes.

scholarly journals Investigation of Tenascin Expression in Endometriosis

2012 ◽  
Vol 2012 ◽  
pp. 1-5
Author(s):  
Zehra Sema Ozkan ◽  
Hasan Cilgin ◽  
Remzi Atilgan ◽  
Mehmet Simsek ◽  
Bengu Cobanoglu ◽  
...  
Keyword(s):  
Matrix Protein ◽  
Expression Patterns ◽  
Serum Levels ◽  
Extracellular Matrix Protein ◽  
Secretory Phase ◽  
Tissue Samples ◽  
Intense Staining ◽  
Tissue Levels ◽  
Significant Difference ◽  
Ectopic Endometrium

Objective. To evaluate the serum and tissue levels and local expression pattern of tenascin, a high molecular weight extracellular matrix protein, in eutopic and ectopic endometrium from patients with and without endometriosis and to compare the proliferative and secretory phase differences. Materials and Methods. Thirty women with endometriosis and fifteen women without endometriosis undergoing surgery for benign indications were included in the study. Serum and tissue levels and proliferative and secretory phase expression patterns of tenascin in the ectopic and eutopic endometrium were analyzed with immunohistochemistry and immunoassays. The results were compared with Mann-Whitney U test. P values <0.05 were considered as statistically significant. Results. Tenascin expression was detected in both of eutopic and ectopic endometrium of women with and without endometriosis. In immunohistochemical staining, intense staining of tenascin was observed in glandular cells of eutopic and ectopic endometrial tissue samples of both groups during secretory phase (P<0.01). Eutopic and ectopic tissue levels of tenascin were higher than serum tenascin levels only secretory phase (P=0.02). There was no significant difference between groups for tissue and serum levels of tenascin during cycle phases. Conclusion. Tenascin expression showed cyclic change on eutopic and ectopic endometrium.

scholarly journals Distinct Expression Patterns of Osteopontin and Dentin Matrix Protein 1 Genes in Pituitary Gonadotrophs

2019 ◽  
Vol 10 ◽  
Author(s):  
Ivana Bjelobaba ◽  
Marija M. Janjic ◽  
Rafael Maso Prévide ◽  
Daniel Abebe ◽  
Marek Kucka ◽  
...  
Keyword(s):  
Matrix Protein ◽  
Expression Patterns ◽  
Dentin Matrix Protein 1 ◽  
Dentin Matrix Protein ◽  
Dentin Matrix

Inflammation-dependent expression of SPARC during development of chronic pancreatitis in WBN/Kob rats and a microarray gene expression analysis

2009 ◽  
Vol 38 (2) ◽  
pp. 196-204 ◽  
Author(s):  
T. Reding ◽  
U. Wagner ◽  
A. B. Silva ◽  
L-K. Sun ◽  
M. Bain ◽  
...  
Keyword(s):  
Gene Expression ◽  
Chronic Pancreatitis ◽  
Chronic Inflammation ◽  
Wistar Rats ◽  
Matrix Protein ◽  
Expression Patterns ◽  
Acinar Cells ◽  
Extracellular Matrix Protein ◽  
Human Pancreas ◽  
Inflammatory Genes

The pathophysiology of human chronic pancreatitis is not well understood and difficult to follow on a molecular basis. Therefore, we used a rat model [Wistar-Bonn/Kobori (WBN/Kob)] that exhibits spontaneous chronic inflammation and fibrosis in the pancreas. Using microarrays we compared gene expression patterns in the pancreas during development of inflammation and fibrosis of WBN/Kob rats with age-matched healthy Wistar rats. The extracellular matrix protein SPARC (secreted protein, acidic, and rich in cysteines) and other transcripts of inflammatory genes were quantified by real-time PCR, and some were localized by immunohistochemistry. When pancreatic inflammation becomes obvious at the age of 16 wk, several hundred genes are increased between 3- and 50-fold in WBN/Kob rats compared with healthy Wistar rats. Proteins produced by acinar cells and characteristic for inflammation, e.g., pancreatitis-associated protein, are highly upregulated. Other proteins, derived from infiltrating inflammatory cells and from activated stellate cells (fibrosis) such as collagens and fibronectins are also significantly upregulated. SPARC was localized to acinar cells where it increased in the vicinity of inflammatory foci. However, acinar expression of SPARC was lost during destruction of acinar cells. In human pancreatic specimens with chronic pancreatitis, SPARC exhibited a similar expression profile. During chronic inflammation and fibrosis in the WBN/Kob rat, inflammatory genes, growth factors, and structural genes exhibit a high increase of expression. A temporal profile including pre- and postinflammatory phases indicates a concurrent activation of inflammatory and fibrotic changes. Inflammation dependent expression of SPARC appears to be lost during acinar-to-duct metaplasia both in rat and human pancreas.

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