Molecular wiring of glucose oxidase enzyme with Mn polypyridine complex on MWCNT modified electrode surface and its bio-electrocatalytic oxidation and glucose sensing

2020 ◽  
pp. 249-262
Author(s):  
Natarajan Saravanan ◽  
Annamalai Senthil Kumar
Keyword(s):  
Glucose Oxidase ◽  
Modified Electrode ◽  
Electrode Surface ◽  
Electrocatalytic Oxidation ◽  
Glucose Sensing ◽  
Oxidase Enzyme ◽  
Molecular Wiring

Evidence of short-range electron transfer of a redox enzyme on graphene oxide electrodes

2014 ◽  
Vol 16 (33) ◽  
pp. 17426-17436 ◽  
Author(s):  
Marccus V. A. Martins ◽  
Andressa R. Pereira ◽  
Roberto A. S. Luz ◽  
Rodrigo M. Iost ◽  
Frank N. Crespilho
Keyword(s):  
Electron Transfer ◽  
Graphene Oxide ◽  
Glucose Oxidase ◽  
Electrode Surface ◽  
Short Range ◽  
Redox Enzyme ◽  
Oxide Electrodes ◽  
Oxidase Enzyme ◽  
Graphene Oxide Sheets

Graphene oxide sheets provide short-range electron transfer from the glucose oxidase enzyme to the electrode surface.

scholarly journals Carbon nanofiber modified with osmium based redox polymer for glucose sensing

2017 ◽  
Vol 7 (4) ◽  
pp. 181-191 ◽  
Author(s):  
Amos Mugweru ◽  
Reaz Mahmud ◽  
Kartik Ghosh ◽  
Adam Wanekaya
Keyword(s):  
Glucose Oxidase ◽  
Carbon Nanofibers ◽  
Carbon Nanofiber ◽  
Energy Dispersive Spectrometer ◽  
High Sensitivity ◽  
Glucose Sensing ◽  
Redox Polymer ◽  
Glucose Determination ◽  
Km Value ◽  
Oxidase Enzyme

Electrochemical detection of glucose was performed on carbon nanofibers containing an osmium based redox polymer and using glucose oxidase enzyme. Redox polymer assembled on the nanofibers provided a more stable support that preserved enzyme activity and promoted the electrical communication to the glassy carbon electrode. The morphologies, structures, and electrochemical behavior of the redox polymer modified nanofibers were characterized by scanning electron microscope, energy dispersive spectrometer and voltammetry. The glucose oxidase showed excellent communication with redox polymer as observed with the increased activity toward glucose. Both cyclic voltammetry and amperometry showed a linear response with glucose concentration.  The linear range for glucose determination was from 1 to 12 mM with a relatively high sensitivity of 0.20±0.01 μA mM−1 for glucose oxidase in carbon nanofibers and 0.10±0.01 μA mM−1 without carbon nanofibers. The apparent Michaelis–Menten constant (Km) for glucose oxidase with carbon nanofibers was 0.99 mM. On the other hand, the Km value for the glucose oxidase without the nanofibers was 4.90 mM.

scholarly journals Highly Sensitive Voltammetric Glucose Biosensor Based on Glucose Oxidase Encapsulated in a Chitosan/Kappa-Carrageenan/Gold Nanoparticle Bionanocomposite

2018 ◽  
Author(s):  
Nicole Jaffrezic-Renault ◽  
Ilhem Rassas ◽  
Mohamed Braiek ◽  
Anne Bonhomme ◽  
François Bessueille ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
Modified Electrode ◽  
Gold Electrode ◽  
Polyelectrolyte Complex ◽  
Glucose Sensing ◽  
Glucose Biosensor ◽  
Good Reproducibility ◽  
Highly Sensitive ◽  
Kappa Carrageenan ◽  
Higher Sensitivity

In this work, an enzymatic sensor, based on a bionanocomposite film consisting of a polyelectrolyte complex (PEC) [Chitosan/kappa-carrageenan] doped with gold nanoparticles (AuNPs) encapsulating glucose oxidase (GOD) deposited on a gold electrode (Au) for glucose sensing, is described. Using the electrocatalytic synergy of AuNPs and GOD as a model of enzyme, the variation of the current (µA) as a function of the log of the glucose concentration (log [glucose]), shows 3 times higher sensitivity for the modified electrode (283.9) compared to that of the PEC/GOD modified electrode (93.7), with a detection limit of about 5 µM and a linearity range between 10µM and 7mM. The response of the PEC/AuNPs/GOD based biosensor also presents good reproducibility, stability and negligible interfering effects from ascorbic acid, uric acid, urea and creatinine. The applicability of the PEC/AuNPs/GOD based biosensor was tested in glucose-spiked saliva samples and acceptable recovery rates were obtained.

Electrosynthesis and electrochemical characteristics of 2,2′-(4,5-dihydroxy-3-methoxy-1,2-phenylene)bis(3-oxo-3-phenylpropanenitrile): application as a mediator for determination of hydroxylamine at a carbon nanotube modified electrode surface

2014 ◽  
Vol 6 (15) ◽  
pp. 5999-6008 ◽  
Author(s):  
Hamid R. Zare ◽  
Milad Tashkili ◽  
Hossein Khoshro ◽  
Davood Nematollahi ◽  
Ali Benvidi
Keyword(s):  
Carbon Nanotube ◽  
Glassy Carbon Electrode ◽  
Modified Electrode ◽  
Glassy Carbon ◽  
Electrode Surface ◽  
Electrocatalytic Oxidation ◽  
Electrochemical Characteristics ◽  
Carbon Electrode ◽  
Modified Glassy Carbon Electrode

Electrosynthesis and electrochemical characteristics of an electrodeposited DMPP film on a multi-wall carbon nanotube modified glassy carbon electrode and its role as a mediator for electrocatalytic oxidation of hydroxylamine.

Glucose Oxidase–graphene–chitosan modified electrode for direct electrochemistry and glucose sensing

2009 ◽  
Vol 25 (4) ◽  
pp. 901-905 ◽  
Author(s):  
Xinhuang Kang ◽  
Jun Wang ◽  
Hong Wu ◽  
Ilhan A. Aksay ◽  
Jun Liu ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
Modified Electrode ◽  
Direct Electrochemistry ◽  
Glucose Sensing

scholarly journals Highly Sensitive Voltammetric Glucose Biosensor Based on Glucose Oxidase Encapsulated in a Chitosan/Kappa-Carrageenan/Gold Nanoparticle Bionanocomposite

Sensors ◽  
2019 ◽  
Vol 19 (1) ◽  
pp. 154 ◽  
Author(s):  
Ilhem Rassas ◽  
Mohamed Braiek ◽  
Anne Bonhomme ◽  
Francois Bessueille ◽  
Guy Raffin ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
Modified Electrode ◽  
Gold Electrode ◽  
Polyelectrolyte Complex ◽  
Glucose Sensing ◽  
Glucose Biosensor ◽  
Good Reproducibility ◽  
Highly Sensitive ◽  
Kappa Carrageenan ◽  
Higher Sensitivity

In this work, an enzymatic sensor, based on a bionanocomposite film consisting of a polyelectrolyte complex (PEC) (Chitosan/kappa-carrageenan) doped with gold nanoparticles (AuNPs) encapsulating glucose oxidase (GOD) deposited on a gold electrode (Au) for glucose sensing, is described. Using the electrocatalytic synergy of AuNPs and GOD as a model of enzyme, the variation of the current (µA) as a function of the log of the glucose concentration (log [glucose]), shows three times higher sensitivity for the modified electrode (283.9) compared to that of the PEC/GOD modified electrode (93.7), with a detection limit of about 5 µM and a linearity range between 10 µM and 7 mM. The response of the PEC/AuNPs/GOD based biosensor also presents good reproducibility, stability, and negligible interfering effects from ascorbic acid, uric acid, urea, and creatinine. The applicability of the PEC/AuNPs/GOD based biosensor was tested in glucose-spiked saliva samples and acceptable recovery rates were obtained.

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