scholarly journals Site-specific analysis of the Asp- and Glu-ADP-ribosylated proteome by quantitative mass spectrometry

2019 ◽  
pp. 301-321 ◽  
Author(s):  
Peng Li ◽  
Yuanli Zhen ◽  
Yonghao Yu
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Mass Spectrometry ◽  
Site Specific ◽  
Specific Analysis

scholarly journals Global and site‐specific analysis of protein glycosylation in complex biological systems with Mass Spectrometry

2019 ◽  
Vol 38 (4-5) ◽  
pp. 356-379 ◽  
Author(s):  
Haopeng Xiao ◽  
Fangxu Sun ◽  
Suttipong Suttapitugsakul ◽  
Ronghu Wu
Keyword(s):  
Mass Spectrometry ◽  
Biological Systems ◽  
Protein Glycosylation ◽  
Site Specific ◽  
Specific Analysis

Site-specific analysis of theN-glycans on murine polymeric immunoglobulin A using liquid chromatography/electrospray mass spectrometry

1995 ◽  
Vol 30 (12) ◽  
pp. 1679-1686 ◽  
Author(s):  
Eddy A. Kragten ◽  
Aldert A. Bergwerff ◽  
Jan van Oostrum ◽  
Dieter R. Müller ◽  
Wilhelm J. Richter
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Electrospray Mass Spectrometry ◽  
Immunoglobulin A ◽  
Site Specific ◽  
Specific Analysis

The challenge of detecting modifications on proteins

2020 ◽  
Vol 64 (1) ◽  
pp. 135-153 ◽  
Author(s):  
Lauren Elizabeth Smith ◽  
Adelina Rogowska-Wrzesinska
Keyword(s):  
Mass Spectrometry ◽  
Protein Function ◽  
Chemical Properties ◽  
Lysine Acetylation ◽  
Mass Determination ◽  
Post Translational Modifications ◽  
Site Specific ◽  
The Common

Abstract Post-translational modifications (PTMs) are integral to the regulation of protein function, characterising their role in this process is vital to understanding how cells work in both healthy and diseased states. Mass spectrometry (MS) facilitates the mass determination and sequencing of peptides, and thereby also the detection of site-specific PTMs. However, numerous challenges in this field continue to persist. The diverse chemical properties, low abundance, labile nature and instability of many PTMs, in combination with the more practical issues of compatibility with MS and bioinformatics challenges, contribute to the arduous nature of their analysis. In this review, we present an overview of the established MS-based approaches for analysing PTMs and the common complications associated with their investigation, including examples of specific challenges focusing on phosphorylation, lysine acetylation and redox modifications.

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