A New Method to Determine the Transmembrane Conformation of Substrates in Intramembrane Proteolysis by Deep-UV Resonance Raman Spectroscopy

2017 ◽  
pp. 207-228 ◽  
Author(s):  
J.W. Cooley ◽  
A. Abdine ◽  
M. Brown ◽  
J. Chavez ◽  
B. Lada ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Resonance Raman Spectroscopy ◽  
Resonance Raman ◽  
New Method ◽  
Intramembrane Proteolysis ◽  
Uv Resonance Raman ◽  
Uv Resonance Raman Spectroscopy ◽  
Deep Uv

scholarly journals Monitoring membrane protein structural changes and interactions via deep UV resonance Raman spectroscopy

2015 ◽  
Author(s):  
◽  
Mia C. Brown
Keyword(s):  
Membrane Proteins ◽  
Structural Changes ◽  
Resonance Raman Spectroscopy ◽  
Resonance Raman ◽  
The Body ◽  
Intramembrane Proteolysis ◽  
Parkinson’S Diseases ◽  
Uv Resonance Raman ◽  
Uv Resonance Raman Spectroscopy ◽  
Deep Uv

Membrane proteins perform a variety of functions within our cells. They transport nutrients and waste across the lipid barrier, transmit signals from one part of the body to another, and run our immune system. However, despite their ubiquitous and vital presence in all organisms, relatively little is known about this class of proteins compared to their soluble counterparts. Intramembrane proteolysis is a process involving membrane proteins that occurs in all biological organisms and has garnered particular interest due to its involvement in various disease pathologies, such as Alzheimer's and Parkinson's Diseases. In this work I have set out to use deep UV resonance Raman (DUVRR) spectroscopy to characterize structural and environmental transitions of proteins and applied the results to studies involving intramembrane proteolysis in an effort to better understand the key concepts behind it.

Simultaneous Observation of Peptide Backbone Lipid Solvation and α-Helical Structure by Deep-UV Resonance Raman Spectroscopy

ChemBioChem ◽  
2011 ◽  
Vol 12 (14) ◽  
pp. 2125-2128 ◽  
Author(s):  
Christopher M. Halsey ◽  
Jian Xiong ◽  
Olayinka O. Oshokoya ◽  
Jeanette A. Johnson ◽  
Sandip Shinde ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Resonance Raman Spectroscopy ◽  
Resonance Raman ◽  
Helical Structure ◽  
Simultaneous Observation ◽  
Peptide Backbone ◽  
Uv Resonance Raman ◽  
Uv Resonance Raman Spectroscopy ◽  
Deep Uv
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