Separation of Two Distinct O-Glycoforms of Human IgA1 by Serial Lectin Chromatography Followed by Mass Spectrometry O-Glycan Analysis

2017 ◽  
pp. 61-75 ◽  
Author(s):  
S. Lehoux ◽  
T. Ju
Keyword(s):  
Mass Spectrometry ◽  
Glycan Analysis ◽  
Lectin Chromatography

scholarly journals N-Glycosylation profiling of intact target proteins by high-resolution mass spectrometry (MS) and glycan analysis using ion mobility-MS/MS

The Analyst ◽  
2020 ◽  
Vol 145 (5) ◽  
pp. 1737-1748
Author(s):  
Alessandro Quaranta ◽  
Maya Spasova ◽  
Elena Passarini ◽  
Isabella Karlsson ◽  
Lorena Ndreu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quality Control ◽  
High Resolution ◽  
Ion Mobility ◽  
High Resolution Mass Spectrometry ◽  
Target Proteins ◽  
Intact Proteins ◽  
Glycan Analysis ◽  
High Resolution Mass ◽  
Resolution Mass

Glycosylation characterization could lead to the discovery of biomarkers and is crucial in quality control of biopharmaceuticals. Here we present a method to quantify glycoforms on intact proteins, with parallel glycan identification by IMS-MS/MS.

scholarly journals Non-derivatized glycan analysis by reverse phase liquid chromatography and ion mobility-mass spectrometry

The Analyst ◽  
2015 ◽  
Vol 140 (10) ◽  
pp. 3335-3338 ◽  
Author(s):  
Nichole M. Lareau ◽  
Jody C. May ◽  
John A. McLean
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Ion Mobility ◽  
Ion Mobility Mass Spectrometry ◽  
Reverse Phase ◽  
Simple Method ◽  
Glycan Analysis ◽  
Reverse Phase Liquid Chromatography ◽  
Phase Liquid ◽  
Phase Column

A simple method for the analysis of non-derivatized glycans using a reverse phase column on a liquid chromatography-ion mobility-mass spectrometry (LC-IM-MS) instrument.

Online nanoliquid chromatography–mass spectrometry and nanofluorescence detection for high-resolution quantitative N-glycan analysis

2012 ◽  
Vol 423 (1) ◽  
pp. 153-162 ◽  
Author(s):  
Hakan Kalay ◽  
Martino Ambrosini ◽  
Patrick H.C. van Berkel ◽  
Paul W.H.I. Parren ◽  
Yvette van Kooyk ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Glycan Analysis ◽  
Chromatography Mass Spectrometry ◽  
Nanoliquid Chromatography
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