scholarly journals Inserting Extrahelical Structures into Long DNA Substrates for Single-Molecule Studies of DNA Mismatch Repair

2017 ◽  
pp. 221-238 ◽  
Author(s):  
M.W. Brown ◽  
A. de la Torre ◽  
I.J. Finkelstein
Keyword(s):  
Mismatch Repair ◽  
Single Molecule ◽  
Dna Mismatch Repair ◽  
Dna Mismatch ◽  
Single Molecule Studies ◽  
Dna Substrates

scholarly journals Single-Molecule Studies on EXOI Excision during DNA Mismatch Repair

2016 ◽  
Vol 110 (3) ◽  
pp. 63a
Author(s):  
Yongmoon Jeon ◽  
Daehyung Kim ◽  
Juana Martín-López ◽  
Ryanggeun Lee ◽  
Jungsic Oh ◽  
...  
Keyword(s):  
Mismatch Repair ◽  
Single Molecule ◽  
Dna Mismatch Repair ◽  
Dna Mismatch ◽  
Single Molecule Studies

scholarly journals Single molecule studies of DNA mismatch repair

DNA Repair ◽  
2014 ◽  
Vol 20 ◽  
pp. 71-81 ◽  
Author(s):  
Dorothy A. Erie ◽  
Keith R. Weninger
Keyword(s):  
Mismatch Repair ◽  
Single Molecule ◽  
Dna Mismatch Repair ◽  
Dna Mismatch ◽  
Single Molecule Studies

scholarly journals Single-molecule imaging reveals target-search mechanisms during DNA mismatch repair

2012 ◽  
Vol 109 (45) ◽  
pp. E3074-E3083 ◽  
Author(s):  
J. Gorman ◽  
F. Wang ◽  
S. Redding ◽  
A. J. Plys ◽  
T. Fazio ◽  
...  
Keyword(s):  
Mismatch Repair ◽  
Single Molecule ◽  
Dna Mismatch Repair ◽  
Single Molecule Imaging ◽  
Target Search ◽  
Dna Mismatch

scholarly journals Mismatch Repair on the Go: Extending the Mechanical Model of DNA Mismatch Repair through Single Molecule Study

2017 ◽  
Vol 112 (3) ◽  
pp. 514a-515a
Author(s):  
Pengyu Hao ◽  
Ruoyi Qiu ◽  
Dorothy Erie ◽  
Keith Weninger
Keyword(s):  
Mismatch Repair ◽  
Single Molecule ◽  
Mechanical Model ◽  
Dna Mismatch Repair ◽  
Dna Mismatch

scholarly journals Toward Single Molecule FRET Studies of DNA Mismatch Repair in Live Bacteria

2019 ◽  
Vol 116 (3) ◽  
pp. 139a
Author(s):  
Pengning Xu ◽  
Andrew Hensley ◽  
Edward Chan ◽  
Keith R. Weninger
Keyword(s):  
Mismatch Repair ◽  
Single Molecule ◽  
Dna Mismatch Repair ◽  
Dna Mismatch ◽  
Single Molecule Fret ◽  
Live Bacteria

scholarly journals The unstructured linker arms of MutL enable GATC site incision beyond roadblocks during initiation of DNA mismatch repair

2019 ◽  
Vol 47 (22) ◽  
pp. 11667-11680 ◽  
Author(s):  
Yannicka S N Mardenborough ◽  
Katerina Nitsenko ◽  
Charlie Laffeber ◽  
Camille Duboc ◽  
Enes Sahin ◽  
...  
Keyword(s):  
Mismatch Repair ◽  
Single Molecule ◽  
Genome Stability ◽  
Dna Mismatch Repair ◽  
Dna Mismatch ◽  
Replication Errors ◽  
Dna Backbone ◽  
Dna Strand ◽  
Dna Replication Errors ◽  
Gatc Site

Abstract DNA mismatch repair (MMR) maintains genome stability through repair of DNA replication errors. In Escherichia coli, initiation of MMR involves recognition of the mismatch by MutS, recruitment of MutL, activation of endonuclease MutH and DNA strand incision at a hemimethylated GATC site. Here, we studied the mechanism of communication that couples mismatch recognition to daughter strand incision. We investigated the effect of catalytically-deficient Cas9 as well as stalled RNA polymerase as roadblocks placed on DNA in between the mismatch and GATC site in ensemble and single molecule nanomanipulation incision assays. The MMR proteins were observed to incise GATC sites beyond a roadblock, albeit with reduced efficiency. This residual incision is completely abolished upon shortening the disordered linker regions of MutL. These results indicate that roadblock bypass can be fully attributed to the long, disordered linker regions in MutL and establish that communication during MMR initiation occurs along the DNA backbone.

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