Assessing Coupled Protein Folding and Binding Through Temperature-Dependent Isothermal Titration Calorimetry

2016 ◽  
pp. 23-45 ◽  
Author(s):  
Debashish Sahu ◽  
Monique Bastidas ◽  
Chad W. Lawrence ◽  
William G. Noid ◽  
Scott A. Showalter
Keyword(s):  
Protein Folding ◽  
Isothermal Titration Calorimetry ◽  
Titration Calorimetry ◽  
Temperature Dependent

scholarly journals Modeling of Multivalent Ligand-Receptor Binding Measured by kinITC

Computation ◽  
2019 ◽  
Vol 7 (3) ◽  
pp. 46
Author(s):  
Franziska Erlekam ◽  
Sinaida Igde ◽  
Susanna Röblitz ◽  
Laura Hartmann ◽  
Marcus Weber
Keyword(s):  
Protein Folding ◽  
Kinetic Parameters ◽  
Invariant Subspace ◽  
Isothermal Titration Calorimetry ◽  
Receptor Binding ◽  
Kinetic Data ◽  
Titration Calorimetry ◽  
Subspace Projection ◽  
Binding Process ◽  
Sequential Binding

In addition to the conventional Isothermal Titration Calorimetry (ITC), kinetic ITC (kinITC) not only gains thermodynamic information, but also kinetic data from a biochemical binding process. Moreover, kinITC gives insights into reactions consisting of two separate kinetic steps, such as protein folding or sequential binding processes. The ITC method alone cannot deliver kinetic parameters, especially not for multivalent bindings. This paper describes how to solve the problem using kinITC and an invariant subspace projection. The algorithm is tested for multivalent systems with different valencies.

scholarly journals Thermodynamic characterization of calcium-milk protein interaction by isothermal titration calorimetry

2009 ◽  
Vol 89 (3-4) ◽  
pp. 257-267 ◽  
Author(s):  
Latha-Selvi Canabady-Rochelle ◽  
Christian Sanchez ◽  
Michel Mellema ◽  
Sylvie Banon
Keyword(s):  
Isothermal Titration Calorimetry ◽  
Protein Interaction ◽  
Milk Protein ◽  
Titration Calorimetry ◽  
Thermodynamic Characterization
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