Cryo-electron tomography of SYCP3 fibers under native conditions

2018 ◽  
pp. 347-371 ◽  
Author(s):  
Daniel Bollschweiler ◽  
Laura Radu ◽  
Luca Pellegrini
Keyword(s):  
Electron Tomography ◽  
Cryo Electron Tomography

scholarly journals Determining the Patchwork Lattice of Ebola and Marburg Virus Matrix Layers Using Cryo-Electron Tomography

2021 ◽  
Vol 27 (S1) ◽  
pp. 1884-1884
Author(s):  
William Wan ◽  
Mairi Clarke ◽  
Michael Norris ◽  
Larissa Kolesnikova ◽  
Alexander Koehler ◽  
...  
Keyword(s):  
Electron Tomography ◽  
Marburg Virus ◽  
Cryo Electron Tomography

scholarly journals Tubulin glycylation controls axonemal dynein activity, flagellar beat, and male fertility

Science ◽  
2021 ◽  
Vol 371 (6525) ◽  
pp. eabd4914
Author(s):  
Sudarshan Gadadhar ◽  
Gonzalo Alvarez Viar ◽  
Jan Niklas Hansen ◽  
An Gong ◽  
Aleksandr Kostarev ◽  
...  
Keyword(s):  
Posttranslational Modifications ◽  
Male Fertility ◽  
Electron Tomography ◽  
Structural Basis ◽  
Cellular Functions ◽  
Flagellar Beat ◽  
Cryo Electron Tomography ◽  
Axonemal Dynein ◽  
Dynein Arms ◽  
Cilia And Flagella

Posttranslational modifications of the microtubule cytoskeleton have emerged as key regulators of cellular functions, and their perturbations have been linked to a growing number of human pathologies. Tubulin glycylation modifies microtubules specifically in cilia and flagella, but its functional and mechanistic roles remain unclear. In this study, we generated a mouse model entirely lacking tubulin glycylation. Male mice were subfertile owing to aberrant beat patterns of their sperm flagella, which impeded the straight swimming of sperm cells. Using cryo–electron tomography, we showed that lack of glycylation caused abnormal conformations of the dynein arms within sperm axonemes, providing the structural basis for the observed dysfunction. Our findings reveal the importance of microtubule glycylation for controlled flagellar beating, directional sperm swimming, and male fertility.

scholarly journals Fluorescence-guided lamella fabrication with ENZEL, an integrated cryogenic CLEM solution for the cryo-electron tomography workflow

2021 ◽  
Vol 27 (S1) ◽  
pp. 3234-3235
Author(s):  
Caspar Jonker ◽  
Daan Boltje ◽  
Jacob Hoogenboom ◽  
Arjen Jakobi ◽  
Grant Jensen ◽  
...  
Keyword(s):  
Electron Tomography ◽  
Cryo Electron Tomography
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