Monitoring complete and incomplete abscission in the germ line stem cell lineage of Drosophila ovaries

2017 ◽  
pp. 105-118 ◽  
Author(s):  
J. Mathieu ◽  
J.-R. Huynh
Keyword(s):  
Stem Cell ◽  
Germ Line ◽  
Cell Lineage ◽  
Stem Cell Lineage ◽  
Germ Line Stem Cell

scholarly journals A Misexpression Screen Reveals Effects of bag-of-marbles and TGFβ Class Signaling on the Drosophila Male Germ-Line Stem Cell Lineage

Genetics ◽  
2004 ◽  
Vol 167 (2) ◽  
pp. 707-723 ◽  
Author(s):  
Cordula Schulz ◽  
Amy A. Kiger ◽  
Salli I. Tazuke ◽  
Yukiko M. Yamashita ◽  
Luiz C. Pantalena-Filho ◽  
...  
Keyword(s):  
Stem Cell ◽  
Germ Line ◽  
Cell Lineage ◽  
Male Germ Line ◽  
Stem Cell Lineage ◽  
Germ Line Stem Cell ◽  
Bag Of Marbles

144. MAELSTROM - A PROTEIN THAT IS ESSENTIAL FOR SPERMATOGENESIS AND TRANSPOSABLE REPRESSION IS EXPRESSED IN ADULT OVARY OF MAMMALS AND BIRDS

2010 ◽  
Vol 22 (9) ◽  
pp. 62
Author(s):  
S. Lim ◽  
E. Tsend-Ayush ◽  
R. Kortschak ◽  
C. Ricciardelli ◽  
M. Oehler ◽  
...  
Keyword(s):  
Germ Cells ◽  
Germ Line ◽  
In Situ Hybridisation ◽  
Computational Modelling ◽  
Cell Lineage ◽  
Cumulus Cells ◽  
Rnai Pathway ◽  
Stem Cell Lineage ◽  
Evolutionarily Conserved ◽  
Germ Line Stem Cell

Maelstrom (MAEL) is a highly evolutionarily conserved protein located at the perinuclear structure of animal germ cells called nuage. The MAEL protein contains HMG and Tudor domains and associates with components of the piRNA and RNAi pathways and chromatin remodelling factors. Recent work has shown that MAEL is required for the differentiation of the germ-line stem cell lineage and for the retroposon repression. In mouse, Mael is expressed in male germ cells and is essential for spermatogenesis and retroposon suppression. We have investigated the evolution of the Mael gene in mammals and birds. As expected the gene is highly conserved in all three mammalian lineages and in chicken. Interestingly, the platypus MAEL has exclusive changes in the DnaQ-H 3’-5’ exonuclease domain and computational modelling suggested that these changes may affect the folding of the protein. Expression analysis revealed that the Mael gene is transcribed in testis but also in adult ovaries of chicken, platypus, mouse and human. In situ hybridisation of the Mael transcript on ovary sections of mouse and platypus shows that gene expression is found in pre-antral and antral follicles. The data so far also showed some differences in the expression pattern between mouse and platypus. In mouse, we detected transcript in oocyte, granulosa cells and cumulus cells whereas in the platypus we only observed expression in oocyte. Earlier work demonstrated that Drosophila Mael mutant ovaries had mislocalisation of the RNAi pathway proteins, Dicer and Argonaute2. It is well known that RNAi pathyway is involved in the repression of transposon in the testis and ovary across animal kingdom. As a key component of the RNAi pathway, MAEL is reported to co-localise and interact with MILI and MIWI proteins. These finding may suggest a role of MAEL in retroposon control in ovary and folliculogenesis.

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