Immunofluorescent staining of septins in primary cilia

2016 ◽  
pp. 269-283 ◽  
Author(s):  
M.S. Kim ◽  
C.D. Froese ◽  
H. Xie ◽  
W.S. Trimble
Keyword(s):  
Primary Cilia ◽  
Immunofluorescent Staining

scholarly journals Exposure to 16 Hz Pulsed Electromagnetic Fields Protect the Structural Integrity of Primary Cilia and Associated TGF-β Signaling in Osteoprogenitor Cells Harmed by Cigarette Smoke

2021 ◽  
Vol 22 (13) ◽  
pp. 7036
Author(s):  
Yangmengfan Chen ◽  
Romina H. Aspera-Werz ◽  
Maximilian M. Menger ◽  
Karsten Falldorf ◽  
Michael Ronniger ◽  
...  
Keyword(s):  
Cell Viability ◽  
Cigarette Smoke ◽  
Electromagnetic Fields ◽  
Primary Cilia ◽  
Structural Integrity ◽  
Immunofluorescent Staining ◽  
Pulsed Electromagnetic Fields ◽  
Migration Assay ◽  
Daily Exposure ◽  
Pulsed Electromagnetic

Cigarette smoking (CS) is one of the main factors related to avoidable diseases and death across the world. Cigarette smoke consists of numerous toxic compounds that contribute to the development of osteoporosis and fracture nonunion. Exposure to pulsed electromagnetic fields (PEMF) was proven to be a safe and effective therapy to support bone fracture healing. The aims of this study were to investigate if extremely low frequency (ELF-) PEMFs may be beneficial to treat CS-related bone disease, and which effect the duration of the exposure has. In this study, immortalized human mesenchymal stem cells (SCP-1 cells) impaired by 5% cigarette smoke extract (CSE) were exposed to ELF-PEMFs (16 Hz) with daily exposure ranging from 7 min to 90 min. Cell viability, adhesion, and spreading were evaluated by Sulforhodamine B, Calcein-AM staining, and Phalloidin-TRITC/Hoechst 33342 staining. A migration assay kit was used to determine cell migration. Changes in TGF-β signaling were evaluated with an adenoviral Smad2/3 reporter assay, RT-PCR, and Western blot. The structure and distribution of primary cilia were analyzed with immunofluorescent staining. Our data indicate that 30 min daily exposure to a specific ELF-PEMF most effectively promoted cell viability, enhanced cell adhesion and spreading, accelerated migration, and protected TGF-β signaling from CSE-induced harm. In summary, the current results provide evidence that ELF-PEMF can be used to support early bone healing in patients who smoke.

Studies on the Binding of Proteins to the Human Platelet Surface: Relation to Platelet Activation

1985 ◽  
Vol 53 (03) ◽  
pp. 360-365 ◽  
Author(s):  
Gerhard K M Endresen ◽  
Øystein Førre
Keyword(s):  
Platelet Aggregation ◽  
Lupus Erythematosus ◽  
Immune Complexes ◽  
Immunofluorescent Staining ◽  
Induce Platelet Aggregation ◽  
Platelet Surface ◽  
Platelet Aggregometry ◽  
Surface Staining ◽  
Alpha1 Antitrypsin ◽  
Induced Aggregation

SummarySeveral antibody fractions and sera from patients with rheumatoid arthritis, systemic lupus erythematosus and chronic idiopathic thrombocytopenic purpura were examined for their ability to bind to normal platelets using immunofluorescent staining techniques. Platelet aggregometry was used to study the activating capacity of the samples.Both C1q, C1s, C1 inactivator, fibrinogen, factor VIII-related antigen, alpha1-acid glycoprotein, alpha1-antitrypsin, beta2-micro- globulin and isoantigens A and B, as well as fibronectin and plasminogen were found on the platelet surface. Only antibodies to C1q, C1s and beta2-microglobulin were able to induce platelet aggregation. Sera containing immune complexes or platelet autoantibodies revealed positive surface staining for IgG, or for IgG and IgM. These sera also induced aggregation of platelets. Sera not containing immune complexes or autoantibodies gave negative staining and aggregation results. Thus, only some of the ligand receptor interactions were able to induce platelet aggregation.

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