scholarly journals Strategies for analyzing cardiac phenotypes in the zebrafish embryo

2016 ◽  
pp. 335-368 ◽  
Author(s):  
A.R. Houk ◽  
D. Yelon
Keyword(s):  
Zebrafish Embryo

The zebrafish embryo model in environmental risk assessment—applications beyond acute toxicity testing

2008 ◽  
Vol 15 (5) ◽  
pp. 394-404 ◽  
Author(s):  
Stefan Scholz ◽  
Stephan Fischer ◽  
Ulrike Gündel ◽  
Eberhard Küster ◽  
Till Luckenbach ◽  
...  
Keyword(s):  
Risk Assessment ◽  
Acute Toxicity ◽  
Environmental Risk ◽  
Environmental Risk Assessment ◽  
Zebrafish Embryo ◽  
Toxicity Testing ◽  
Acute Toxicity Testing

scholarly journals Toxic or Not Toxic, That Is the Carbon Quantum Dot’s Question: A Comprehensive Evaluation with Zebrafish Embryo, Eleutheroembryo, and Adult Models

Polymers ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1598
Author(s):  
Chih-Yu Chung ◽  
Yu-Ju Chen ◽  
Chia-Hui Kang ◽  
Hung-Yun Lin ◽  
Chih-Ching Huang ◽  
...  
Keyword(s):  
Zebrafish Embryo ◽  
Comprehensive Evaluation ◽  
Aquatic Organisms ◽  
Carbon Quantum Dots ◽  
Adult Zebrafish ◽  
Fish Embryo ◽  
Wide Range ◽  
Different Types ◽  
Low Toxicity

Carbon quantum dots (CQDs) are emerging novel nanomaterials with a wide range of applications and high biocompatibility. However, there is a lack of in-depth research on whether CQDs can cause acute or long-term adverse reactions in aquatic organisms. In this study, two different types of CQDs prepared by ammonia citrate and spermidine, namely CQDAC and CQDSpd, were used to evaluate their biocompatibilities. In the fish embryo acute toxicity test (FET), the LD50 of CQDAC and CQDSpd was about 500 and 100 ppm. During the stage of eleutheroembryo, the LD50 decreased to 340 and 55 ppm, respectively. However, both CQDs were quickly eliminated from embryo and eleutheroembryo, indicating a lack of bioaccumulation. Long-term accumulation of CQDs was also performed in this study, and adult zebrafish showed no adverse effects in 12 weeks. In addition, there was no difference in the hatchability and deformity rates of offspring produced by adult zebrafish, regardless of whether they were fed CQDs or not. The results showed that both CQDAC and CQDSpd have low toxicity and bioaccumulation to zebrafish. Moreover, the toxicity assay developed in this study provides a comprehensive platform to assess the impacts of CQDs on aquatic organisms in the future.

scholarly journals Effective processing and evaluation of chemical imaging data with respect to morphological features of the zebrafish embryo

2021 ◽  
Author(s):  
Katharina Halbach ◽  
Timothy Holbrook ◽  
Thorsten Reemtsma ◽  
Stephan Wagner
Keyword(s):  
Inductively Coupled Plasma ◽  
Zebrafish Embryo ◽  
Statistical Significance ◽  
Software Tool ◽  
Chemical Imaging ◽  
Imaging Data ◽  
Inductively Coupled ◽  
Spatially Resolved ◽  
Icp Ms ◽  
Plasma Mass Spectrometry

AbstractA workflow was developed and implemented in a software tool for the automated combination of spatially resolved laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) data and data on the morphology of the biological tissue. Making use of a recently published biological annotation software, FishImager automatically assigns the biological feature as regions of interest (ROIs) and overlays them with the quantitative LA-ICP-MS data. Furthermore, statistical tools including cluster algorithms can be applied to the elemental intensity data and directly compared with the ROIs. This is effectively visualized in heatmaps. This allows gaining statistical significance on distribution and co-localization patterns. Finally, the biological functions of the assigned ROIs can then be easily linked with elemental distributions. We demonstrate the versatility of FishImager with quantitative LA-ICP-MS data of the zebrafish embryo tissue. The distribution of natural elements and xenobiotics is analyzed and discussed. With the help of FishImager, it was possible to identify compartments affected by toxicity effects or biological mechanisms to eliminate the xenobiotic. The presented workflow can be used for clinical and ecotoxicological testing, for example. Ultimately, it is a tool to simplify and reproduce interpretations of imaging LA-ICP-MS data in many applications. Graphical abstract

Toxicity of polymer‐modified CuS nanoclusters on zebrafish embryo development

2021 ◽  
Author(s):  
Qiuping Zhang ◽  
Lifeng Wang ◽  
Qian Gao ◽  
Xinge Zhang ◽  
Yushuang Lin ◽  
...  
Keyword(s):  
Embryo Development ◽  
Zebrafish Embryo

Developmental and neurobehavioral assessment of low-dose N-nitrosodimethylamine (NDMA) using zebrafish embryo bioassay

2021 ◽  
Vol 770 ◽  
pp. 144748
Author(s):  
Yajie Zhang ◽  
Bin Zhang ◽  
Ting Xu ◽  
Xinyue Yang ◽  
Sheng Wei ◽  
...  
Keyword(s):  
Zebrafish Embryo ◽  
Low Dose
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