scholarly journals Analyzing kinesin motor domain translocation in cultured hippocampal neurons

2016 ◽  
pp. 217-232 ◽  
Author(s):  
Rui Yang ◽  
Marvin Bentley ◽  
Chung-Fang Huang ◽  
Gary Banker
Keyword(s):  
Hippocampal Neurons ◽  
Motor Domain ◽  
Kinesin Motor ◽  
Cultured Hippocampal Neurons

scholarly journals A novel split kinesin assay identifies motor proteins that interact with distinct vesicle populations

2012 ◽  
Vol 198 (4) ◽  
pp. 749-761 ◽  
Author(s):  
Brian Jenkins ◽  
Helena Decker ◽  
Marvin Bentley ◽  
Julie Luisi ◽  
Gary Banker
Keyword(s):  
Cell Biology ◽  
Hippocampal Neurons ◽  
Systematic Approach ◽  
Living Cells ◽  
Motor Domain ◽  
Challenging Problem ◽  
Experimental Paradigm ◽  
Kinesin Motor ◽  
Selective Transport ◽  
Cultured Hippocampal Neurons

Identifying the kinesin motors that interact with different vesicle populations is a longstanding and challenging problem with implications for many aspects of cell biology. Here we introduce a new live-cell assay to assess kinesin–vesicle interactions and use it to identify kinesins that bind to vesicles undergoing dendrite-selective transport in cultured hippocampal neurons. We prepared a library of “split kinesins,” comprising an axon-selective kinesin motor domain and a series of kinesin tail domains that can attach to their native vesicles; when the split kinesins were assembled by chemical dimerization, bound vesicles were misdirected into the axon. This method provided highly specific results, showing that three Kinesin-3 family members—KIF1A, KIF13A, and KIF13B—interacted with dendritic vesicle populations. This experimental paradigm allows a systematic approach to evaluate motor–vesicle interactions in living cells.

scholarly journals Dihydrotestosterone activates CREB signaling in cultured hippocampal neurons

2009 ◽  
Vol 1298 ◽  
pp. 1-12 ◽  
Author(s):  
Thuy-Vi V. Nguyen ◽  
Mingzhong Yao ◽  
Christian J. Pike
Keyword(s):  
Hippocampal Neurons ◽  
Cultured Hippocampal Neurons
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