scholarly journals Stem and progenitor cells in skeletal development

2019 ◽  
pp. 1-24 ◽  
Author(s):  
Noriaki Ono ◽  
Deepak H. Balani ◽  
Henry M. Kronenberg
Keyword(s):  
Progenitor Cells ◽  
Skeletal Development ◽  
Stem And Progenitor Cells

scholarly journals miR17~92 is essential for the survival of hematopoietic stem and progenitor cells by restraining pro-apoptotic BIM

2018 ◽  
Author(s):  
Kerstin Brinkmann ◽  
Craig Hyland ◽  
Carolyn A de Graaf ◽  
Andreas Strasser ◽  
Warren S Alexander ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Skeletal Development ◽  
Well Being ◽  
Micro Rna ◽  
Dramatic Reduction ◽  
Hematopoietic Stem ◽  
Hematopoietic System ◽  
Cellular Processes ◽  
Adult Mice ◽  
Stem And Progenitor Cells

SummaryThe micro RNA cluster miR17~92, also known as oncomiR-1, impacts diverse cellular processes, such as cell survival and proliferation. Constitutive loss of miR17~92 in mice causes severe defects in skeletal development, organ development and hematopoiesis, resulting in early post-natal lethality. The critical functions of miR17~92 in a fully developed animal have not yet been explored. Here we show that deletion of miR17~92 in adult mice had no impact on their lifespan or general well-being. However, detailed analysis of the hematopoietic system in mice, revealed a dramatic reduction in all mature hematopoietic lineages, which was due to the loss of early hematopoietic stem/progenitor cells (HSPCs). Strikingly, the concomitant loss of the pro-apoptotic BH3-only protein BIM rescued the loss of the HSPCs and all of their differentiated progeny that was caused by the deletion of miR17~92. These findings demonstrate that miR17~92 is critical for the survival of HSPCs by restraining the activity of the pro-apoptotic BH3-only protein BIM.

scholarly journals Hypertrophic Chondrocytes Serve as a Reservoir for Unique Marrow Associated Skeletal Stem and Progenitor Cells, Osteoblasts, and Adipocytes During Skeletal Development

2021 ◽  
Author(s):  
Jason T. Long ◽  
Abigail Leinroth ◽  
Yihan Liao ◽  
Yinshi Ren ◽  
Anthony J. Mirando ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Rna Sequencing ◽  
Bone Development ◽  
Skeletal Development ◽  
Primary Source ◽  
Hypertrophic Chondrocytes ◽  
Immunofluorescent Staining ◽  
Growth Plate Chondrocytes ◽  
Stem And Progenitor Cells

Skeletal stem and progenitor cells (SSPCs) reside within niches localized to the intramedullary bone marrow and periosteal tissues surrounding bones, with most being capable of becoming osteoblasts, chondrocytes, and adipocytes during bone development and/or regeneration. SSPCs within the periosteum can give rise to intramedullary SSPCs, osteoblasts, osteocytes, and adipocytes during bone development; however, whether they are the sole source of these cells remains to be determined. Growth plate chondrocytes contribute to the osteoblast lineage and trabecular bone formation; however, the cellular process used to achieve this is unknown. We utilized hypertrophic chondrocyte genetic reporter mouse models combined with single cell RNA-sequencing, immunofluorescent staining, and bulk RNA-sequencing approaches to determine that hypertrophic chondrocytes undergo a process of dedifferentiation to generate unique bone marrow associated SSPC populations that likely serve as a primary source of osteogenic cells during skeletal development, while also contributing to the adipogenic lineage with age.

Flow cytometric enumeration and immunophenotyping of hematopoietic stem and progenitor cells

2001 ◽  
Vol 38 (2) ◽  
pp. 139-147
Author(s):  
Jan W. Gratama ◽  
D. Robert Sutherland ◽  
Michael Keeney
Keyword(s):  
Progenitor Cells ◽  
Hematopoietic Stem ◽  
Flow Cytometric ◽  
Stem And Progenitor Cells
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