Genetic Analysis of Human Preimplantation Embryos

2016 ◽  
pp. 421-447 ◽  
Author(s):  
S. Garcia-Herrero ◽  
A. Cervero ◽  
E. Mateu ◽  
P. Mir ◽  
M.E. Póo ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Preimplantation Embryos ◽  
Human Preimplantation Embryos

scholarly journals Transmission ratio distortion in the myotonic dystrophy locus in human preimplantation embryos

2006 ◽  
Vol 14 (3) ◽  
pp. 299-306 ◽  
Author(s):  
Nicola L Dean ◽  
J Concepción Loredo-Osti ◽  
T Mary Fujiwara ◽  
Kenneth Morgan ◽  
Seang Lin Tan ◽  
...  
Keyword(s):  
Myotonic Dystrophy ◽  
Transmission Ratio Distortion ◽  
Preimplantation Embryos ◽  
Transmission Ratio ◽  
Ratio Distortion ◽  
Human Preimplantation Embryos

scholarly journals Biological and Clinical Significance of Mosaicism in Human Preimplantation Embryos

2021 ◽  
Vol 9 (2) ◽  
pp. 18
Author(s):  
Ioanna Bouba ◽  
Elissavet Hatzi ◽  
Paris Ladias ◽  
Prodromos Sakaloglou ◽  
Charilaos Kostoulas ◽  
...  
Keyword(s):  
Assisted Reproduction ◽  
Basic Research ◽  
Blastocyst Stage ◽  
Preimplantation Embryos ◽  
New Approach ◽  
Assisted Reproduction Technology ◽  
Clinical Utilization ◽  
Art Practices ◽  
In Utero Development ◽  
Human Preimplantation Embryos

Applications and indications of assisted reproduction technology are expanding, but every new approach is under scrutiny and thorough consideration. Recently, groups of assisted reproduction experts have presented data that support the clinical use of mosaic preimplantation embryos at the blastocyst stage, previously excluded from transfer. In the light of published contemporary studies, with or without clinical outcomes, there is growing evidence that mosaic embryos have the capacity for further in utero development and live birth. Our in-depth discussion will enable readers to better comprehend current developments. This expansion into the spectrum of ART practices requires further evidence and further theoretical documentation, basic research, and ethical support. Therefore, if strict criteria for selecting competent mosaic preimplantation embryos for further transfer, implantation, fetal growth, and healthy birth are applied, fewer embryos will be excluded, and more live births will be achieved. Our review aims to discuss the recent literature on the transfer of mosaic preimplantation embryos. It also highlights controversies as far as the clinical utilization of preimplantation embryos concerns. Finally, it provides the appropriate background to elucidate and highlight cellular and genetic aspects of this novel direction.

scholarly journals Quantitative and qualitative changes of mitochondria in human preimplantation embryos

2017 ◽  
Vol 34 (5) ◽  
pp. 573-580 ◽  
Author(s):  
Shu Hashimoto ◽  
Naoharu Morimoto ◽  
Masaya Yamanaka ◽  
Hiroshi Matsumoto ◽  
Takayuki Yamochi ◽  
...  
Keyword(s):  
Preimplantation Embryos ◽  
Human Preimplantation Embryos ◽  
Qualitative Changes

scholarly journals Effects of pyruvate and glucose on the development of human preimplantation embryos in vitro

Reproduction ◽  
1993 ◽  
Vol 99 (1) ◽  
pp. 87-95 ◽  
Author(s):  
J. Conaghan ◽  
A. H. Handyside ◽  
R. M. L. Winston ◽  
H. J. Leese
Keyword(s):  
Preimplantation Embryos ◽  
Human Preimplantation Embryos

scholarly journals Comparison of gene expression in fresh and frozen–thawed human preimplantation embryos

Reproduction ◽  
2012 ◽  
Vol 144 (5) ◽  
pp. 569-582 ◽  
Author(s):  
Lisa Shaw ◽  
Sharon F Sneddon ◽  
Daniel R Brison ◽  
Susan J Kimber
Keyword(s):  
Gene Expression ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Developmental Expression ◽  
Preimplantation Embryos ◽  
Human Embryos ◽  
Molecular Events ◽  
Reliable Source ◽  
Human Preimplantation Embryos ◽  
Early Human

Identification and characterisation of differentially regulated genes in preimplantation human embryonic development are required to improve embryo quality and pregnancy rates in IVF. In this study, we examined expression of a number of genes known to be critical for early development and compared expression profiles in individual preimplantation human embryos to establish any differences in gene expression in fresh compared to frozen–thawed embryos used routinely in IVF. We analysed expression of 19 genes by cDNA amplification followed by quantitative real-time PCR in a panel of 44 fresh and frozen–thawed human preimplantation embryos. Fresh embryos were obtained from surplus early cleavage stage embryos and frozen–thawed embryos from cryopreserved 2PN embryos. Our aim was to determine differences in gene expression between fresh and frozen–thawed human embryos, but we also identified differences in developmental expression patterns for particular genes. We show that overall gene expression among embryos of the same stage is highly variable and our results indicate that expression levels between groups did differ and differences in expression of individual genes was detected. Our results show that gene expression from frozen–thawed embryos is more consistent when compared with fresh, suggesting that cryopreserved embryos may represent a reliable source for studying the molecular events underpinning early human embryo development.

Sign in / Sign up

Export Citation Format

Share Document