Section 8 update: Stable isotope probing of nucleic acids to identify active microbial populations

2012 ◽  
Keyword(s):  
Stable Isotope ◽  
Nucleic Acids ◽  
Stable Isotope Probing ◽  
Microbial Populations ◽  
Section 8

Dynamics of soil microbial populations involved in 2,4-D biodegradation revealed by FAME-based Stable Isotope Probing

2009 ◽  
Vol 41 (1) ◽  
pp. 77-85 ◽  
Author(s):  
Thomas Z. Lerch ◽  
Marie-France Dignac ◽  
Naoise Nunan ◽  
Gérard Bardoux ◽  
Enrique Barriuso ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Stable Isotope Probing ◽  
Microbial Populations ◽  
Soil Microbial

scholarly journals Targeted metagenomics of active microbial populations with stable-isotope probing

2016 ◽  
Vol 41 ◽  
pp. 1-8 ◽  
Author(s):  
Sara Coyotzi ◽  
Jennifer Pratscher ◽  
J Colin Murrell ◽  
Josh D Neufeld
Keyword(s):  
Stable Isotope ◽  
Stable Isotope Probing ◽  
Microbial Populations

scholarly journals Syntrophomonadaceae-Affiliated Species as Active Butyrate-Utilizing Syntrophs in Paddy Field Soil

2011 ◽  
Vol 77 (11) ◽  
pp. 3884-3887 ◽  
Author(s):  
Pengfei Liu ◽  
Qiongfen Qiu ◽  
Yahai Lu
Keyword(s):  
Stable Isotope ◽  
Nucleic Acids ◽  
Paddy Field ◽  
Stable Isotope Probing ◽  
Field Soil ◽  
Content Type ◽  
Paddy Field Soil

ABSTRACTDNA-based stable-isotope probing was applied to identify the active microorganisms involved in syntrophic butyrate oxidation in paddy field soil. After 14 and 21 days of incubation with [U-13C]butyrate, the bacterialSyntrophomonadaceaeand the archaealMethanosarcinaceaeandMethanocellalesincorporated substantial amounts of13C label into their nucleic acids. Unexpectedly, members of thePlanctomycetesandChloroflexiwere also labeled with13C by yet-unclear mechanisms.

scholarly journals Proteomic Stable Isotope Probing Reveals Taxonomically Distinct Patterns in Amino Acid Assimilation by Coastal Marine Bacterioplankton

mSystems ◽  
2016 ◽  
Vol 1 (2) ◽  
Author(s):  
Samuel Bryson ◽  
Zhou Li ◽  
Jennifer Pett-Ridge ◽  
Robert L. Hettich ◽  
Xavier Mayali ◽  
...  
Keyword(s):  
Amino Acid ◽  
Stable Isotope ◽  
Organic Carbon ◽  
Stable Isotope Probing ◽  
Microbial Populations ◽  
Cellular Functions ◽  
Marine Systems ◽  
Spatial And Temporal Scales ◽  
Functional Roles ◽  
Microbial Carbon

ABSTRACT An estimated 50 gigatons of carbon is annually fixed within marine systems, of which heterotrophic microbial populations process nearly half. These communities vary in composition and activity across spatial and temporal scales, so understanding how these changes affect global processes requires the delineation of functional roles for individual members. In a step toward ascertaining these roles, we applied proteomic stable isotope probing to quantify the assimilation of organic carbon from DFAAs into microbial protein biomass, since the turnover of DFAAs accounts for a substantial fraction of marine microbial carbon metabolism that is directed into biomass production. We conducted experiments at two coastal North Pacific locations and found taxonomically distinct responses. This approach allowed us to compare amino acid assimilation by specific bacterioplankton populations and characterize their allocation of this substrate among cellular functions. Heterotrophic marine bacterioplankton are a critical component of the carbon cycle, processing nearly a quarter of annual primary production, yet defining how substrate utilization preferences and resource partitioning structure microbial communities remains a challenge. In this study, proteomic stable isotope probing (proteomic SIP) was used to characterize population-specific assimilation of dissolved free amino acids (DFAAs), a major source of dissolved organic carbon for bacterial secondary production in aquatic environments. Microcosms of seawater collected from Newport, Oregon, and Monterey Bay, California, were incubated with 1 µM 13C-labeled amino acids for 15 and 32 h. The taxonomic compositions of microcosm metaproteomes were highly similar to those of the sampled natural communities, with Rhodobacteriales, SAR11, and Flavobacteriales representing the dominant taxa. Analysis of 13C incorporation into protein biomass allowed for quantification of the isotopic enrichment of identified proteins and subsequent determination of differential amino acid assimilation patterns between specific bacterioplankton populations. Proteins associated with Rhodobacterales tended to have a significantly high frequency of 13C-enriched peptides, opposite the trend for Flavobacteriales and SAR11 proteins. Rhodobacterales proteins associated with amino acid transport and metabolism had an increased frequency of 13C-enriched spectra at time point 2. Alteromonadales proteins also had a significantly high frequency of 13C-enriched peptides, particularly within ribosomal proteins, demonstrating their rapid growth during incubations. Overall, proteomic SIP facilitated quantitative comparisons of DFAA assimilation by specific taxa, both between sympatric populations and between protein functional groups within discrete populations, allowing an unprecedented examination of population level metabolic responses to resource acquisition in complex microbial communities. IMPORTANCE An estimated 50 gigatons of carbon is annually fixed within marine systems, of which heterotrophic microbial populations process nearly half. These communities vary in composition and activity across spatial and temporal scales, so understanding how these changes affect global processes requires the delineation of functional roles for individual members. In a step toward ascertaining these roles, we applied proteomic stable isotope probing to quantify the assimilation of organic carbon from DFAAs into microbial protein biomass, since the turnover of DFAAs accounts for a substantial fraction of marine microbial carbon metabolism that is directed into biomass production. We conducted experiments at two coastal North Pacific locations and found taxonomically distinct responses. This approach allowed us to compare amino acid assimilation by specific bacterioplankton populations and characterize their allocation of this substrate among cellular functions.

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