Visualization Based on Molecular Tagging Methods

2011 ◽  
Keyword(s):  
Molecular Tagging ◽  
Tagging Methods

scholarly journals Photoisomeric molecular tagging velocimetry with CCVJ

2021 ◽  
Vol 6 (4) ◽  
Author(s):  
Markus J. Schmidt ◽  
Benno Käslin ◽  
Thomas Rösgen
Keyword(s):  
Molecular Tagging Velocimetry ◽  
Molecular Tagging

scholarly journals Optimization of a WGA-Free Molecular Tagging-Based NGS Protocol for CTCs Mutational Profiling

2020 ◽  
Vol 21 (12) ◽  
pp. 4364
Author(s):  
Giuseppa De Luca ◽  
Barbara Cardinali ◽  
Lucia Del Mastro ◽  
Sonia Lastraioli ◽  
Franca Carli ◽  
...  
Keyword(s):  
Substantial Reduction ◽  
Breast Cancer Patients ◽  
Dna Templates ◽  
Molecular Tagging ◽  
Mutational Profiling ◽  
Optimized Protocol ◽  
Set Up ◽  
Detection Of Mutations ◽  
Almost All ◽  
Next Generation Sequencing Ngs

Molecular characterization of Circulating Tumor Cells (CTCs) is still challenging, despite attempts to minimize the drawbacks of Whole Genome Amplification (WGA). In this paper, we propose a Next-Generation Sequencing (NGS) optimized protocol based on molecular tagging technology, in order to detect CTCs mutations while skipping the WGA step. MDA-MB-231 and MCF-7 cell lines, as well as leukocytes, were sorted into pools (2–5 cells) using a DEPArray™ system and were employed to set up the overall NGS procedure. A substantial reduction of reagent volume for the preparation of libraries was performed, in order to fit the limited DNA templates directly derived from cell lysates. Known variants in TP53, KRAS, and PIK3CA genes were detected in almost all the cell line pools (35/37 pools, 94.6%). No additional alterations, other than those which were expected, were found in all tested pools and no mutations were detected in leukocytes. The translational value of the optimized NGS workflow is confirmed by sequencing CTCs pools isolated from eight breast cancer patients and through the successful detection of variants. In conclusion, this study shows that the proposed NGS molecular tagging approach is technically feasible and, compared to traditional NGS approaches, has the advantage of filtering out the artifacts generated during library amplification, allowing for the reliable detection of mutations and, thus, making it highly promising for clinical use.

Molecular tagging of the dwarf BREIZH (Bzh) gene in Brassica napus

1995 ◽  
Vol 91 (5) ◽  
pp. 756-761 ◽  
Author(s):  
N. Foisset ◽  
R. Delourme ◽  
P. Barret ◽  
M. Renard
Keyword(s):  
Brassica Napus ◽  
Molecular Tagging

Quantitative NO2 molecular tagging velocimetry at 500 kHz frame rate

2010 ◽  
Vol 97 (22) ◽  
pp. 221103 ◽  
Author(s):  
Naibo Jiang ◽  
Munetake Nishihara ◽  
Walter R. Lempert
Keyword(s):  
Frame Rate ◽  
Molecular Tagging Velocimetry ◽  
Molecular Tagging
Sign in / Sign up

Export Citation Format

Share Document