Production of Leukocyte Migration Inhibition Factor by Lymphocytes of Larynx Cancer Patients Stimulated by Laryngeal Carcinoma Solubilized Membrane Antigens

1982 ◽  
Vol 68 (1) ◽  
pp. 39-46 ◽  
Author(s):  
Giorgio Cortesina ◽  
Giovanni Patrick Cavallo ◽  
Fabio Beatrice ◽  
Alberto Sartoris ◽  
Mario Bussi ◽  
...  
Keyword(s):  
Laryngeal Carcinoma ◽  
Normal Mucosa ◽  
Leukocyte Migration ◽  
Migration Inhibition Factor ◽  
Migration Inhibition ◽  
Inhibition Factor ◽  
Histocompatibility Complex ◽  
Laryngeal Mucosa ◽  
Leukocyte Migration Inhibition ◽  
Membrane Antigens

The production of leukocyte migration inhibition factor (LIF) from lymphocytes after stimulation with 3 M KCl soluble tumor and normal mucosa extracts was investigated in 30 patients with laryngeal carcinoma at different development stages and in 30 normal donors. The experiments were performed in heterologous and autologous systems. In heterologous systems 3 M KCl tumor extracts induced LIF production by heterologous lymphocytes from patients in 91 % of the cases, and normal mucosa extracts induced LIF production by heterologous lymphocytes from patients in 73 % of the cases and from normal donors in 90 % of the cases. In autologous systems 3 M KCl tumor extracts induced LIF production by autologous lymphocytes from the same patients in 65 % of the cases, whereas the normal laryngeal mucosa extracts induced LIF production by the same autologous lymphocytes in the 6 % of the cases. The high positivity percentage of the test in heterologous systems could be related to differences in the major histocompatibility complex. The 65 % test positivity in autologous systems using tumor extracts could be related to the presence of tumor associated antigens.

Impaired Cell-Mediated Immune Response in Patients with Congenital Rubella: Correlation with Gestational Age at Time of Infection

PEDIATRICS ◽  
1979 ◽  
Vol 64 (5) ◽  
pp. 620-626
Author(s):  
Elena Buimovici-Klein ◽  
Paul B. Lang ◽  
Philip R. Ziring ◽  
Louis Z. Cooper
Keyword(s):  
Immune Response ◽  
Gestational Age ◽  
Rubella Virus ◽  
Lymphocyte Transformation ◽  
Leukocyte Migration ◽  
Migration Inhibition Factor ◽  
Migration Inhibition ◽  
Inhibition Factor ◽  
Congenital Rubella ◽  
Leukocyte Migration Inhibition

Lymphocyte transformation, interferon, and leukocyte migration inhibition factor synthesis were studied in purified lymphocyte cultures for 20 children with congenital rubella and 18 healthy children (seven susceptible and 11 immune to rubella). Lymphocyte transformation after phytohemagglutinin stimulation was significantly lower in children with congenital rubella as compared to healthy controls. Responses to purified rubella virus were absent in the susceptible controls and absent or at least two times lower in congenital rubella children than in immune controls. After purified rubella virus stimulation, leukocyte migration inhibition factor production was detected in all immune controls, but in none of the susceptible controls, or the congenital rubella-infected children. The results varied with gestational age of intrauterine infection: the impairment of cellular immune response, both after phytohemagglutinin or rubella virus stimulation, was more severe in the children infected in the first two months than in the latter stages of gestation.

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