Human monocytes bind to two cytokine-induced adhesive ligands on cultured human endothelial cells: endothelial-leukocyte adhesion molecule-1 and vascular cell adhesion molecule-1

Blood ◽  
1991 ◽  
Vol 77 (10) ◽  
pp. 2266-2271 ◽  
Author(s):  
T Carlos ◽  
N Kovach ◽  
B Schwartz ◽  
M Rosa ◽  
B Newman ◽  
...  
Keyword(s):  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Leukocyte Adhesion ◽  
Vascular Cell Adhesion ◽  
Vascular Cell ◽  
Leukocyte Adhesion Molecule ◽  
Endothelial Leukocyte Adhesion Molecule ◽  
Adhesive Proteins ◽  
Cell Adhesion Molecule 1 ◽  
Monocyte Adherence

Abstract Vascular cell adhesion molecule-1 (VCAM-1) and endothelial-leukocyte adhesion molecule-1 (ELAM-1) are adhesive proteins induced on endothelium by cytokines. We examined the contribution of these adhesive proteins to human peripheral blood monocyte adherence to endothelium using transfected Chinese hamster ovary (CHO) cells stably expressing these proteins and monoclonal antibodies (MoAbs) to ELAM-1, VCAM-1, or CD49d/CD29 (VLA-4), the leukocyte receptor for VCAM-1. Monocytes bound to CHO cells transfected with cDNA of ELAM-1 or VCAM-1. Binding to ELAM-1 was inhibited by MoAb to ELAM-1 and binding to VCAM-1 was inhibited by MoAb to VCAM-1 or the alpha-chain of very late activation antigen-4 (VLA-4) (CD49d). Additive inhibition of adherence to unstimulated human umbilical vein endothelium (HUVE) was observed when monocytes were pretreated with both MoAb to CD49d and MoAb to CD18, the common beta-chain of the leukocyte beta 2 integrin receptors. Adherence of monocytes to HUVE stimulated by recombinant human tumor necrosis factor-alpha was not reduced by MoAbs to CD18, CD49d, or ELAM- 1 when used singly, but combinations of these MoAbs produced significant inhibition. We conclude that multiple receptor-ligand systems are involved in monocyte adherence to endothelium.

A blocking monoclonal antibody to endothelial-leukocyte adhesion molecule-1 (ELAM1)

1990 ◽  
Vol 171 (1) ◽  
pp. 348-353 ◽  
Author(s):  
Christopher Benjamin ◽  
Irene Dougas ◽  
Gloria Chi-Rosso ◽  
Stefan Luhowskyj ◽  
Margaret Rosa ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Adhesion Molecule ◽  
Leukocyte Adhesion ◽  
Leukocyte Adhesion Molecule ◽  
Endothelial Leukocyte Adhesion Molecule
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