scholarly journals FluorescenceIn SituHybridization (FISH)-Based Karyotyping Reveals Rapid Evolution of Centromeric and Subtelomeric Repeats in Common Bean (Phaseolus vulgaris) and Relatives

2016 ◽  
Vol 6 (4) ◽  
pp. 1013-1022 ◽  
Author(s):  
Aiko Iwata-Otsubo ◽  
Brittany Radke ◽  
Seth Findley ◽  
Brian Abernathy ◽  
C. Eduardo Vallejos ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Rapid Evolution ◽  
Subtelomeric Repeats

Organization of a Solatium brevidens repetitive sequence related to the TGRI subtelomeric repeats of Lycopersicon esculentum

1994 ◽  
Vol 89 (1) ◽  
pp. 1-8 ◽  
Author(s):  
J. Preiszner ◽  
I. Takács ◽  
M. Bilgin ◽  
J. Györgyey ◽  
D. Dudits ◽  
...  
Keyword(s):  
Lycopersicon Esculentum ◽  
Repetitive Sequence ◽  
Subtelomeric Repeats

scholarly journals Organization of subtelomeric repeats in Plasmodium berghei.

1990 ◽  
Vol 10 (5) ◽  
pp. 2423-2427 ◽  
Author(s):  
E Dore ◽  
T Pace ◽  
M Ponzi ◽  
L Picci ◽  
C Frontali
Keyword(s):  
Plasmodium Berghei ◽  
Tandem Repeats ◽  
Telomeric Sequence ◽  
Chromosome Size ◽  
Base Pairs ◽  
Telomeric Sequences ◽  
Subtelomeric Repeats

Several (but not all) Plasmodium berghei chromosomes bear in the subtelomeric position a cluster of 2.3-kilobase (kb) tandem repeats. The 2.3-kb unit contains 160 base pairs of telomeric sequence. The resulting subtelomeric structure is one in which stretches of telomeric sequences are periodically spaced by a 2.1-kb reiterated sequence. This periodic organization of internal telomeric sequences might be related to chromosome-size polymorphisms involving the loss or addition of subtelomeric 2.3-kb units.

Characterization of chromosome ends on the basis of the structure of TrsA subtelomeric repeats in rice (Oryza sativa L.)

2008 ◽  
Vol 280 (1) ◽  
pp. 19-24 ◽  
Author(s):  
Hiroshi Mizuno ◽  
Jianzhong Wu ◽  
Yuichi Katayose ◽  
Hiroyuki Kanamori ◽  
Takuji Sasaki ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Subtelomeric Repeats

scholarly journals Prospects of telomere-to-telomere assembly in barley: analysis of sequence gaps in the MorexV3 reference genome

2021 ◽  
Author(s):  
Pavla Navrátilová ◽  
Helena Toegelová ◽  
Zuzana Tulpová ◽  
Yi-Tzu Kuo ◽  
Nils Stein ◽  
...  
Keyword(s):  
Ribosomal Dna ◽  
Reference Genome ◽  
Plant Chromosomes ◽  
Satellite Repeats ◽  
Dna Repeat ◽  
Optical Map ◽  
Long Read ◽  
Subtelomeric Repeats ◽  
The Difference ◽  
Almost All

The first gapless, telomere-to-telomere (T2T) sequence assemblies of plant chromosomes were reported recently. However, sequence assemblies of most plant genomes remain fragmented. Only recent breakthroughs in accurate long-read sequencing have made it possible to achieve highly contiguous sequence assemblies with a few tens of contigs per chromosome, i.e. a number small enough to allow for a systematic inquiry into the causes of the remaining sequence gaps and the approaches and resources needed to close them. Here, we analyze sequence gaps in the current reference genome sequence of barley cv. Morex (MorexV3). Optical map and sequence raw data, complemented by ChIP-seq data for centromeric histone variant CENH3, were used to estimate the abundance of centromeric, ribosomal DNA and subtelomeric repeats in the barley genome. These estimates were compared with copy numbers in the MorexV3 pseudomolecule sequence. We found that almost all centromeric sequences and 45S ribosomal DNA repeat arrays were absent from the MorexV3 pseudomolecules and that the majority of sequence gaps can be attributed to assembly breakdown in long stretches of satellite repeats. However, missing sequences cannot fully account for the difference between assembly size and flow cytometric genome size estimates. We discuss the prospects of gap closure with ultra-long sequence reads.

Organization of subtelomeric repeats in Plasmodium berghei

1990 ◽  
Vol 10 (5) ◽  
pp. 2423-2427
Author(s):  
E Dore ◽  
T Pace ◽  
M Ponzi ◽  
L Picci ◽  
C Frontali
Keyword(s):  
Plasmodium Berghei ◽  
Tandem Repeats ◽  
Telomeric Sequence ◽  
Chromosome Size ◽  
Base Pairs ◽  
Telomeric Sequences ◽  
Subtelomeric Repeats

Several (but not all) Plasmodium berghei chromosomes bear in the subtelomeric position a cluster of 2.3-kilobase (kb) tandem repeats. The 2.3-kb unit contains 160 base pairs of telomeric sequence. The resulting subtelomeric structure is one in which stretches of telomeric sequences are periodically spaced by a 2.1-kb reiterated sequence. This periodic organization of internal telomeric sequences might be related to chromosome-size polymorphisms involving the loss or addition of subtelomeric 2.3-kb units.

scholarly journals Telomerase-Independent Stabilization of Short Telomeres in Trypanosoma brucei

2006 ◽  
Vol 26 (13) ◽  
pp. 4911-4919 ◽  
Author(s):  
Oliver Dreesen ◽  
George A. M. Cross
Keyword(s):  
Trypanosoma Brucei ◽  
Germ Cells ◽  
Telomere Maintenance ◽  
Genomic Integrity ◽  
Cloning And Sequencing ◽  
Telomere Elongation ◽  
Subtelomeric Repeats ◽  
Rapid Elongation ◽  
Human Telomerase

ABSTRACT In cancer cells and germ cells, shortening of chromosome ends is prevented by telomerase. Telomerase-deficient cells have a replicative life span, after which they enter senescence. Senescent cells can give rise to survivors that maintain chromosome ends through recombination-based amplification of telomeric or subtelomeric repeats. We found that in Trypanosoma brucei, critically short telomeres are stable in the absence of telomerase. Telomere stabilization ensured genomic integrity and could have implications for telomere maintenance in human telomerase-deficient cells. Cloning and sequencing revealed 7 to 27 TTAGGG repeats on stabilized telomeres and no changes in the subtelomeric region. Clones with short telomeres were used to study telomere elongation dynamics, which differed dramatically at transcriptionally active and silent telomeres, after restoration of telomerase. We propose that transcription makes the termini of short telomeres accessible for rapid elongation by telomerase and that telomere elongation in T. brucei is not regulated by a protein-counting mechanism. Many minichromosomes were lost after long-term culture in the absence of telomerase, which may reflect their different mitotic segregation properties.

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