fatvg encodes a new localized RNA that uses a 25-nucleotide element (FVLE1) to localize to the vegetal cortex of Xenopus oocytes

Development ◽  
1999 ◽  
Vol 126 (22) ◽  
pp. 4943-4953 ◽  
Author(s):  
A.P. Chan ◽  
M. Kloc ◽  
L.D. Etkin
Keyword(s):  
Cell Fate ◽  
Xenopus Oocytes ◽  
Single Copy ◽  
Biological Functions ◽  
Embryonic Patterning ◽  
Cis Acting ◽  
Maternal Transcript ◽  
Vegetal Cortex ◽  
Mitochondrial Cloud ◽  
Localization Elements

Vegetally localized transcripts have been implicated in a number of important biological functions, including cell fate determination and embryonic patterning. We have isolated a cDNA, fatvg, which encodes a localized maternal transcript that exhibits a localization pattern reminiscent of Vg1 mRNA. fatvg is the homologue of a mammalian gene expressed in adipose tissues. The fatvg transcript, unlike Vg1 which localizes strictly through the Late pathway, also associates with the mitochondrial cloud that is characteristic of the METRO or Early pathway. This suggests that fatvg mRNA may utilize both the METRO and Late pathways to localize to the vegetal cortex during oogenesis. We have dissected the cis-acting localization elements of fatvg mRNA and compared these elements with Vg1 mRNA. Our results indicate that, like most localized RNAs, in a variety of systems, transcripts of fatvg contain localization elements in the 3′UTR. The 3′UTR of fatvg mRNA contains multiple elements that are able to function independently; however, it functions most efficiently when all of the elements are present. We have defined a short 25-nucleotide element that can direct vegetal localization as a single copy. This element differs in sequence from previously described Vg1 localization elements, suggesting that different localization elements are involved in the localization of RNAs through the Late pathway.

Expression of zebrafish cyp11a1 as a maternal transcript and in yolk syncytial layer

2002 ◽  
Vol 2 (3-4) ◽  
pp. 219-222 ◽  
Author(s):  
Hwei-Jan Hsu ◽  
Peihung Hsiao ◽  
Ming-Wei Kuo ◽  
Bon-chu Chung
Keyword(s):  
Yolk Syncytial Layer ◽  
Maternal Transcript

scholarly journals Joint action of two RNA degradation pathways controls the timing of maternal transcript elimination at the midblastula transition in Drosophila melanogaster

1999 ◽  
Vol 18 (9) ◽  
pp. 2610-2620 ◽  
Author(s):  
Arash Bashirullah ◽  
Susan R. Halsell ◽  
Ramona L. Cooperstock ◽  
Malgorzata Kloc ◽  
Angelo Karaiskakis ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Joint Action ◽  
Rna Degradation ◽  
Degradation Pathways ◽  
Midblastula Transition ◽  
Maternal Transcript

scholarly journals The Maternal Transcript for Truncated Voltage-Dependent Ca2+ Channels in the Ascidian Embryo: A Potential Suppressive Role in Ca2+ Channel Expression

2001 ◽  
Vol 230 (2) ◽  
pp. 258-277 ◽  
Author(s):  
Ryugo Okagaki ◽  
Hiroko Izumi ◽  
Toshiaki Okada ◽  
Hitoshi Nagahora ◽  
Koichi Nakajo ◽  
...  
Keyword(s):  
Maternal Transcript ◽  
Voltage Dependent ◽  
Channel Expression ◽  
Ca2 Channels ◽  
Ascidian Embryo

Delay in maternal transcript degradation in ovine embryos derived from low competence oocytes

2018 ◽  
Vol 85 (5) ◽  
pp. 427-439 ◽  
Author(s):  
Laura Masala ◽  
Federica Ariu ◽  
Luisa Bogliolo ◽  
Emanuela Bellu ◽  
Sergio Ledda ◽  
...  
Keyword(s):  
Maternal Transcript

scholarly journals Heparan Sulfate Biosynthesis in Zebrafish

2020 ◽  
Vol 69 (1) ◽  
pp. 49-60
Author(s):  
Beata Filipek-Górniok ◽  
Judith Habicher ◽  
Johan Ledin ◽  
Lena Kjellén
Keyword(s):  
Biological Activity ◽  
Heparan Sulfate ◽  
Genome Duplication ◽  
Model System ◽  
Zebrafish Genome ◽  
Zebrafish Model ◽  
Functional Studies ◽  
Maternal Transcript ◽  
Golgi Compartment ◽  
Sulfation Pattern

The biosynthesis of heparan sulfate (HS) proteoglycans occurs in the Golgi compartment of cells and will determine the sulfation pattern of HS chains, which in turn will have a large impact on the biological activity of the proteoglycans. Earlier studies in mice have demonstrated the importance of HS for embryonic development. In this review, the enzymes participating in zebrafish HS biosynthesis, along with a description of enzyme mutants available for functional studies, are presented. The consequences of the zebrafish genome duplication and maternal transcript contribution are briefly discussed as are the possibilities of CRISPR/Cas9 methodologies to use the zebrafish model system for studies of biosynthesis as well as proteoglycan biology.

6 CLONING AND EXPRESSION OF BOVINE FACTOR IN THE GERMLINE ALPHA (FIGLA) IN OOCYTES AND EARLY EMBRYOS: A POTENTIAL TARGET OF microRNA-212

2011 ◽  
Vol 23 (1) ◽  
pp. 109 ◽  
Author(s):  
S. K. Tripurani ◽  
K. B. Lee ◽  
G. W. Smith ◽  
J. Yao
Keyword(s):  
Embryonic Development ◽  
Expression Analysis ◽  
Transcriptional Regulator ◽  
Early Embryogenesis ◽  
Luciferase Reporter ◽  
Bovine Embryos ◽  
Cell Stage ◽  
Primordial Follicles ◽  
Maternal Transcript ◽  
In Cells

Factor In the GermLine Alpha (FIGLA), a basic helix-loop-helix transcription factor, was first identified in regulating coordinate expression of zona pellucida genes in mice. It plays a crucial role in the formation of primordial follicles and lack of FIGLA in mice alters the expression of many oocyte specific genes that are required for fertilization and early embryonic survival. The objective of this study was to characterise the expression and regulation of bovine FIGLA during early embryogenesis. The cloned bovine FIGLA cDNA is 660 bp in length, which encodes a protein of 165 amino acids. Expression of bovine FIGLA mRNA is restricted to ovarian tissue and can be detected in fetal ovaries harvested as early as 90 days of gestation when primordial follicles start to form. Expression analysis demonstrated that FIGLA mRNA is abundant in germinal vesicle and metaphase II stage oocytes, as well as in embryos from pronuclear to 8-cell stage, but barely detectable in embryos collected at morula and blastocyst stages, suggesting that FIGLA might be a maternal effect gene. Recent studies in zebrafish have highlighted the importance of non-coding small RNAs (microRNAs) as key regulatory molecules targeting maternal mRNAs for degradation during embryonic development. We hypothesised that FIGLA, as a maternal transcript, is regulated by microRNAs during early embryogenesis. Using microInspector, an algorithm for detection of possible interactions between microRNAs and target mRNA sequences, a microRNA binding site (miR-212) was identified in the 3′-UTR of the bovine FIGLA mRNA. Alignment of the 3′-UTR of FIGLA mRNAs from bovine, human and mouse shows complete conservation of the ‘seed’ region indicating that miR-212 might be a post-transcriptional regulator of FIGLA and the microRNA: mRNA interaction is evolutionary conserved. Expression analysis indicates that bovine miR-212 is expressed in oocytes and tends to increase at the 4-cell and 8-cell stage embryos followed by a decline at morula and blastocyst stages, indicating that miR-212 is presumably of maternal origin and potentially involved in maternal transcript degradation during the maternal-to-embryonic transition. To validate the role of miR-212 in silencing FIGLA, a luciferase reporter assay was performed using HeLa cells. The luciferase activity in cells expressing a luciferase construct containing the entire 3′ UTR of bovine FIGLA was suppressed by ∼40% in the presence of miR-212. We also investigated the stability of FIGLA mRNA in cells transfected with bovine FIGLA expression plasmid in the presence or absence of miR-212. Expression of bovine FIGLA mRNA was significantly reduced in the presence of mir-212 compared to control cells transfected with FIGLA construct alone. In summary, our data establish miR-212 as a potential post-transcriptional regulator of FIGLA during the maternal-to-embryonic transition in bovine embryos. Future studies aim to determine if miR-212 mimic can inhibit endogenous FIGLA expression in bovine embryos and its effect on subsequent embryonic development.

scholarly journals The Drosophila prage Gene, Required for Maternal Transcript Destabilization in Embryos, Encodes a Predicted RNA Exonuclease

2016 ◽  
Vol 6 (6) ◽  
pp. 1687-1693 ◽  
Author(s):  
Jun Cui ◽  
Yun Wei Lai ◽  
Caroline V. Sartain ◽  
Rebecca M. Zuckerman ◽  
Mariana F. Wolfner
Keyword(s):  
Maternal Transcript
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