Magnetic Particles used in a New Approach for Designed Protein Crystallization

CrystEngComm ◽  
2021 ◽  
Author(s):  
Raquel dos Santos ◽  
Maria João Romão ◽  
Ana C A Roque ◽  
Ana Luisa Moreira Carvalho
Keyword(s):  
Structure Determination ◽  
Magnetic Particles ◽  
Protein Crystallization ◽  
Protein Structures ◽  
3D Structure ◽  
New Approach ◽  
X Ray ◽  
X Ray Crystallography ◽  
3D Structure Determination

After more than one hundred and thirty thousand protein structures determined by X-ray crystallography, the challenge of protein crystallization for 3D structure determination remains. In the quest for additives for...

scholarly journals 3D structure determination of native mammalian cells using cryo-FIB and cryo-electron tomography

2012 ◽  
Vol 180 (2) ◽  
pp. 318-326 ◽  
Author(s):  
Ke Wang ◽  
Korrinn Strunk ◽  
Gongpu Zhao ◽  
Jennifer L. Gray ◽  
Peijun Zhang
Keyword(s):  
Structure Determination ◽  
Mammalian Cells ◽  
Electron Tomography ◽  
3D Structure ◽  
Cryo Electron Tomography ◽  
3D Structure Determination

scholarly journals 3D structure determination of amyloid fibrils using solid-state NMR spectroscopy

Methods ◽  
2018 ◽  
Vol 138-139 ◽  
pp. 26-38 ◽  
Author(s):  
Antoine Loquet ◽  
Nadia El Mammeri ◽  
Jan Stanek ◽  
Mélanie Berbon ◽  
Benjamin Bardiaux ◽  
...  
Keyword(s):  
Solid State ◽  
Nmr Spectroscopy ◽  
Structure Determination ◽  
Solid State Nmr ◽  
Amyloid Fibrils ◽  
3D Structure ◽  
Solid State Nmr Spectroscopy ◽  
3D Structure Determination

scholarly journals Accurate inference of the full base-pairing structure of RNA by deep mutational scanning and covariation-induced deviation of activity

2019 ◽  
Author(s):  
Zhe Zhang ◽  
Peng Xiong ◽  
Tongchuan Zhang ◽  
Junfeng Wang ◽  
Jian Zhan ◽  
...  
Keyword(s):  
Human Genome ◽  
Structure Determination ◽  
False Positive ◽  
3D Structure ◽  
Noncoding Rnas ◽  
High Accuracy ◽  
Base Pairing ◽  
Base Pairs ◽  
3D Structure Determination ◽  
Covariation Analysis

ABSTRACTDespite the transcription of noncoding RNAs in 75% of the human genome and their roles in many diseases include cancer, we know very little about them due to lack of structural clues. The centerpiece of the structural clues is the full RNA base-pairing structure of secondary and tertiary contacts that can be precisely obtained only from costly and inefficient 3D structure determination. Here, we performed deep mutational scanning of self-cleaving CPEB3 ribozyme by error-prone PCR and showed that a library of <5×104 single-to-triple mutants is sufficient to infer all 26 including nonhelical and noncanonical base pairs at the precision of a single false positive. The accurate inference, further confirmed by a twister ribozyme, is resulted from covariation analysis by utilizing both functional and nonfunctional variants for unsupervised learning, followed by restrained optimization. The result highlights the usefulness of deep mutational scanning for high-accuracy structural inference.

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