Microcell–Mediated Chromosome Transfer

2012 ◽  
Keyword(s):  
Chromosome Transfer

Chromosome mobilization of Legionella pneumophila with RK2::Mu and Tn5-Mob

1992 ◽  
Vol 38 (7) ◽  
pp. 664-671 ◽  
Author(s):  
Clifford S. Mintz ◽  
Chang Hua Zou
Keyword(s):  
Genetic Mapping ◽  
Key Words ◽  
Legionella Pneumophila ◽  
Transposon Tn5 ◽  
Helper Plasmid ◽  
Chromosome Transfer ◽  
Chromosomal Markers

RK2::Mu plasmids and transposon Tn5-Mob were used to mobilize the Legionella pneumophila chromosome. Plate matings between L. pneumophila donors that contained RK2::Mu plasmids and auxotrophic recipients yielded recombinants at fequencies ranging from 10−6 to 10−7 per recipient for the markers tested. The presence of a Mu insertion in the chromosome of donors that harbored RK2::Mu plasmids increased the frequency of chromosome transfer of certain selected markers as compared with strains that contained RK2::Mu alone. Cotransfer experiments with Mu-containing donors and a thymidine and tryptophan auxotroph failed to reveal any linkage between the thy and trp loci in L. pneumophila. A strain that contained a chromosomal Tn5-Mob insertion and helper plasmid pRK24.4 transferred chromosomal markers at frequencies of 10−7 per recipient. These findings suggest that RK2::Mu plasmids and Tn5-Mob may be useful for genetic mapping experiments with L. pneumophila. Key words: Legionella pneumophila, chromosome transfer, Tn5-Mob, RK2::Mu.

scholarly journals Chromosome transfer inPseudomonas aeruginosamediated by R factors

1971 ◽  
Vol 17 (2) ◽  
pp. 169-172 ◽  
Author(s):  
Vilma A. Stanisich ◽  
B. W. Holloway
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Specific Resistance ◽  
Host Chromosome ◽  
Chromosome Transfer ◽  
R Factors

SUMMARYR factors present inPseudomonas aeruginosastrains of clinical origin can be transferred to other strains ofP. aeruginosaand may act to promote host chromosome transfer. In general, their properties are similar to those R factors in Enterobacteria. The different R factors studied vary with respect to transferability, transfer of specific resistance properties, repressibility, and ability to promote chromosome transfer.

scholarly journals Recalibration of the Pseudomonas aeruginosa Strain PAO Chromosome Map in Time Units Using High-Frequency-of-Recombination Donors

Genetics ◽  
1987 ◽  
Vol 115 (4) ◽  
pp. 611-618
Author(s):  
Kim O'Hoy ◽  
Viji Krishnapillai
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Nalidixic Acid ◽  
High Frequency ◽  
Temperature Sensitive ◽  
Chromosome Transfer ◽  
Chromosome Map ◽  
Entire Chromosome

ABSTRACT High-frequency-of-recombination donors of P. aeruginosa strain PAO were generated using a temperature-sensitive, replication mutant of the IncP-1 plasmid R68, loaded with the transposon Tn2521. Fourteen donors so isolated mobilized the chromosome in a polarized manner from a number of different transfer origins. The donors were used to construct a time of entry map of the entire chromosome and this was achieved by determining the time of entry of 32 randomly dispersed markers in crosses using nalidixic acid to interrupt chromosome transfer. Analysis of the time of entry data enabled the recalibration of the chromosome map to 75 min.

R Plasmids and Bacterial Chromosome Transfer

1980 ◽  
pp. 19-27
Author(s):  
B. W. Holloway ◽  
C. Crowther ◽  
P. Royle ◽  
M. Nayudu
Keyword(s):  
Bacterial Chromosome ◽  
Chromosome Transfer ◽  
R Plasmids
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