Mutant Isolation

Gene Cloning and Mutant Isolation of Subunits of RNA Polymerases in the Yeast Saccharomyces Cerevisiae

Extrachromosomal Elements in Lower Eukaryotes ◽

10.1007/978-1-4684-5251-8_40 ◽

1986 ◽

pp. 519-531 ◽

Cited By ~ 1

Author(s):

Pierre Thuriaux ◽

Carl Mann ◽

Jean-Marie Buhler ◽

Isabelle Treich ◽

Rosmarie Gudenus ◽

...

Keyword(s):

Saccharomyces Cerevisiae ◽

Gene Cloning ◽

Rna Polymerases ◽

Yeast Saccharomyces Cerevisiae ◽

Mutant Isolation

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Encyclopedia of Genetics, Genomics, Proteomics and Informatics ◽

10.1007/978-1-4020-6754-9_11005 ◽

2008 ◽

pp. 1295-1296

Keyword(s):

Mutant Isolation

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Pronuclear fusion failure: an alternate conjugational pathway in Tetrahymena thermophila, induced by vinblastine.

Genetics ◽

10.1093/genetics/118.4.627 ◽

1988 ◽

Vol 118 (4) ◽

pp. 627-636

Author(s):

E P Hamilton ◽

P B Suhr-Jessen ◽

E Orias

Keyword(s):

Essential Role ◽

Tetrahymena Thermophila ◽

Mitotic Division ◽

Whole Genome ◽

Genetic Origins ◽

Mutant Isolation

Abstract Vinblastine is shown to induce pronuclear fusion failure in conjugating Tetrahymena thermophila. In this alternate conjugational pathway gametic pronuclei are exchanged between conjugants but do not fuse. Each pronucleus undergoes one mitotic division to produce a new macro- and micronucleus. Genetic consequences of pronuclear fusion failure include the following: (1) the progeny are whole genome homozygotes with nuclei derived from single meiotic products, and (2) half of the progeny are heterokaryons with micro- and macronuclei of different genetic origins. These facts make this process extremely useful in strain construction and mutant isolation. The induction of pronuclear fusion failure by vinblastine suggests that microtubules play an essential role in pronuclear fusion.

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