Glycogen synthase kinase-3β mediated regulation of matrix metalloproteinase-9 and its involvement in oral squamous cell carcinoma progression and invasion

2017 ◽  
Vol 41 (1) ◽  
pp. 47-60 ◽  
Author(s):  
Kamdeo K. Pramanik ◽  
Siddavaram Nagini ◽  
Abhay K. Singh ◽  
Prajna Mishra ◽  
Tanushree Kashyap ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Matrix Metalloproteinase ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Glycogen Synthase ◽  
Matrix Metalloproteinase 9 ◽  
Glycogen Synthase Kinase ◽  
Glycogen Synthase Kinase 3Β ◽  
Metalloproteinase 9

scholarly journals Matrix metalloproteinase-9 (MMP-9) as prospective histopathological and molecular biomarker for oral squamous cell carcinoma

2018 ◽  
Vol 6 (8) ◽  
pp. 2801
Author(s):  
Bhagyalakshmi Atla ◽  
Sreevalli Prabhakula ◽  
Seeram Satish Kumar ◽  
Lakshmi Saraswathi Boni
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Matrix Metalloproteinase ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Treatment Strategies ◽  
Matrix Metalloproteinase 9 ◽  
P Value ◽  
Female Ratio ◽  
Metalloproteinase 9

Background: Despite the advance of therapeutic approaches, the morbidity and mortality of oral squamous cell carcinoma have not improved significantly. The invasion potential of oral squamous cell carcinoma is conferred by their ability to utilize Matrix metalloproteinase that are produced by the host’s stromal cells. In the present study an effort was made to analyse the role of clinical features and risk factors in the causation of oral squamous cell carcinoma and expression of Matrix metalloproteinase-9 (MMP-9) was studied in various grades of squamous cell carcinoma with its role in metastasis.Methods: The present study is an hospital based prospective study for a period of two years at the department of pathology from August 2014 to July 2016 consisting of 88 cases of oral squamous cell carcinoma. Tissue for H and E sections were fixed in 10% formalin and subjected to routine paraffin embedded processing and stained with hematoxylin and eosin.Results: Total number of cases of oral squamous cell carcinoma analyzed were 88, out of which 65 cases were subjected to MMP 9 expression by random selection on Immunohistochemistry. Majority of cases occurred in the age group of 41-50 years with male: female ratio of 1.4:1. In stage I and II the expression of MMP-9 is low in 37 cases (82.22%) and in stage III the expression is high in 14 cases (70%) with P value <0.05 (significant).Conclusions: Matrix metalloproteinases could act as a therapeutic target and could play an important role in effective treatment strategies of oral cancer.

scholarly journals A circular RNA derived from MMP9 facilitates oral squamous cell carcinoma metastasis through regulation of MMP9 mRNA stability

2019 ◽  
Vol 28 (12) ◽  
pp. 1614-1623 ◽  
Author(s):  
Bing Xia ◽  
Tao Hong ◽  
Xin He ◽  
Xinlan Hu ◽  
Yongbo Gao
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Matrix Metalloproteinase ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Messenger Rna ◽  
Rna Stability ◽  
Circular Rna ◽  
Matrix Metalloproteinase 9 ◽  
Metalloproteinase 9

Emerging evidence demonstrates that dysregulation of circular RNA is linked to tumorigenesis and aggressive progression. However, its role in oral squamous cell carcinoma remains largely uncharacterized. In this study, we identified a novel metastasis-associated circular RNA, circular matrix metalloproteinase 9 (hsa_circ_0001162, a circular RNA derived from matrix metalloproteinase 9), which was remarkably upregulated in oral squamous cell carcinoma and positively correlated with matrix metalloproteinase 9 expression. Patients with high circular matrix metalloproteinase 9 expression were prone to lymph node metastasis and an advanced TNM stage. Importantly, circular matrix metalloproteinase 9 was identified as an efficacious diagnostic and prognostic biomarker for oral squamous cell carcinoma patients. Functional experiments showed that depletion of circular matrix metalloproteinase 9 weakened the migratory and invasive capabilities of oral squamous cell carcinoma cells in vitro as well as inhibited lung metastasis in vivo. Regarding the mechanism, circular matrix metalloproteinase 9 could simultaneously interact with AUF1 and miR-149 to block the inhibitory effect of AUF1 and miR-149 on matrix metalloproteinase 9 3′-untranslated region, resulting in enhanced matrix metalloproteinase 9 messenger RNA stability, thereby facilitating oral squamous cell carcinoma metastasis. Collectively, our data indicate that circular matrix metalloproteinase 9 acts as a metastasis-promoting gene in oral squamous cell carcinoma through regulating the messenger RNA stability of its parental gene. Therapeutic targeting of circular matrix metalloproteinase 9 may be a promising treatment intervention for metastatic oral squamous cell carcinoma patients.

scholarly journals Immunoexpression of Matrix Metalloproteinase-9 in Histopathological tissue samples of oral Squamous Cell Carcinoma

2021 ◽  
Vol 15 (10) ◽  
pp. 2666-2669
Author(s):  
Fiza Shafiq ◽  
Abbas Saleem Khan ◽  
Sajjad Ahmad ◽  
Malik Arshman Khan ◽  
Sikandar Javed , Bajwa ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Cancer ◽  
Oral Squamous Cell Carcinoma ◽  
Matrix Metalloproteinase ◽  
Cell Carcinoma ◽  
Cancer Progression ◽  
Squamous Cell ◽  
Matrix Metalloproteinase 9 ◽  
Healthy Individuals ◽  
Metalloproteinase 9

Background: Oral cancer represents a significant component of the global cancer burden. It develops as a result of multiple risk factors. The main histological form of oral cancer is oral squamous cell carcinoma (OSCC). The disintegration of the extracellular matrix (ECM) is one of the key hallmarks of cancer progression, which stimulates the invasion of tumoral cells into the neighbouring tissue. Matrix metalloproteinases-9 (MMP-9) can degrade different elements of the ECM and basement membrane. Methods: A total of 35 histologically diagnosed cases of OSCC (n=25) and healthy individuals (n=10) as control were included. Immunohistochemical staining was performed on all cases. The results were analysed using SPSS version 20. Results: Increased expression of MMP-9 was observed in OSCC cases (n=23/25; 92%) as compared to healthy individuals. Statistically, a significant relation was observed while comparing the grade of staining intensity and MMP-9 expression among OSCC cases (p=0.001). Conclusion: The present study is planned to assess the immunohistochemical expression of MMP-9 in diagnosed cases of OSCC and healthy individuals. Increased expression of MMP-9 was observed in OSCC as compared to healthy individuals. Evaluation of MMP-9 could be of clinical importance in OSCC and can be used as a diagnostic predictive marker of OSCC. Keywords: Oral squamous cell carcinoma; Matrix Metalloproteinase -9; Immunohistochemistry

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