Diterpenoid natural compound C4 (Crassin) exerts cytostatic effects on triple-negative breast cancer cells via a pathway involving reactive oxygen species

2017 ◽  
Vol 41 (1) ◽  
pp. 35-46 ◽  
Author(s):  
Cathy E. Richards ◽  
Sri H. Vellanki ◽  
Yvonne E. Smith ◽  
Ann M. Hopkins
Keyword(s):  
Breast Cancer ◽  
Reactive Oxygen Species ◽  
Cancer Cells ◽  
Triple Negative Breast Cancer ◽  
Breast Cancer Cells ◽  
Natural Compound ◽  
Triple Negative ◽  
Reactive Oxygen ◽  
Oxygen Species

scholarly journals Pentagamaboronon-0-Sorbitol Induces Apoptosis through Elevation of Reactive Oxygen Species in Triple Negative Breast Cancer Cells

2021 ◽  
Vol 12 (1) ◽  
pp. 46
Author(s):  
Ratna Dwi Ramadani ◽  
Rohmad Yudi Utomo ◽  
Adam Hermawan ◽  
Edy Meiyanto
Keyword(s):  
Breast Cancer ◽  
Reactive Oxygen Species ◽  
Cell Cycle ◽  
Cancer Cells ◽  
Triple Negative Breast Cancer ◽  
Breast Cancer Cells ◽  
Triple Negative ◽  
Oxygen Species ◽  
Apoptosis Induction ◽  
Curcumin Analogue

Breast cancer is the most common type of cancer causing mortality for women due to metastasis. More than 50% of breast cancer patients are suffered lung metastases. One strategy to target the cancerous cell is Boron Neutron Captured Therapy (BNCT) which showed high affinity toward cancer cells and reported to have anti-proliferative as well as anti-metastatic activities. Pentagamaboronon-0 (PGB-0) is a curcumin analogue substance which had reported to exert anticancer activities against Her-2 expressing as well as triple negative breast cancer cells. Despite its great potency as BNCT agent candidate, this compound also exerted several anticancer properties. Complex formation of this substance with sorbitol was achieved to improve the solubility and maximize compound’s delivery to the target cells. This study aimed to investigate the ability of Pentagamaboronon-0-Sorbitol (PGB-0-So) to modulate cell cycle and induce apoptosis especially through the mechanisms of reactive oxygen species (ROS) modulation. The 3-(4,5-dimethylthiazzol-2yl)-2,5-diphenyltetrazolium (MTT) cytotoxicity assay of PGB-0-So against 4T1 breast cancer cell line were found to exert potential effect in dose-dependent manner with lethal concentration (IC50) values of 39 μM. The cytotoxicity of PGB-0-So complex was found to be increased considerably compared with that of PGB-0. Cell cycle modulation identified using propidium iodide (PI) staining showed cell accumulation in S phase following treatment with PGB-0-So. Apoptosis induction assay analyzed using flowcytometer with Annexin V and PI staining on its IC50 dose was found to induce programmed cell death (apoptosis). The sub-IC50 treatment of this compound was also improved the cellular ROS level which also took role in apoptosis induction. These findings suggest that PGB-0-So is potential as an anticancer agent.Keywords: Curcumin analogue, PGB-0-So, Anticancer, 4T1 cell line, ROS modulation.

Resveratrol Induces Reactive Oxygen Species Production and Apoptosis in Breast Cancer Cells

2010 ◽  
Vol 49 ◽  
pp. S92-S93
Author(s):  
Paula Seixas Costa ◽  
Danielly Cristiny Ferraz Costa ◽  
Fabiana Alves Casanova ◽  
Jerson Lima Silva ◽  
Eliane Fialho Oliveira
Keyword(s):  
Breast Cancer ◽  
Reactive Oxygen Species ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Reactive Oxygen Species Production ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Species Production
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