Microarray analysis of apoptosis gene expression in liver injury induced by chronic exposure to arsenic and high-fat diet in male mice

2019 ◽  
Vol 26 (25) ◽  
pp. 26351-26366 ◽  
Author(s):  
Heibatullah Kalantari ◽  
Mohammad Javad Khodayar ◽  
Najmaldin Saki ◽  
Layasadat Khorsandi ◽  
Ali Teymoori ◽  
...  
Keyword(s):  
Gene Expression ◽  
Liver Injury ◽  
Microarray Analysis ◽  
High Fat Diet ◽  
Chronic Exposure ◽  
Male Mice ◽  
High Fat ◽  
Apoptosis Gene

scholarly journals Exposure to Bisphenol-A during Pregnancy Partially Mimics the Effects of a High-Fat Diet Altering Glucose Homeostasis and Gene Expression in Adult Male Mice

PLoS ONE ◽  
2014 ◽  
Vol 9 (6) ◽  
pp. e100214 ◽  
Author(s):  
Marta García-Arevalo ◽  
Paloma Alonso-Magdalena ◽  
Junia Rebelo Dos Santos ◽  
Ivan Quesada ◽  
Everardo M. Carneiro ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bisphenol A ◽  
Glucose Homeostasis ◽  
Adult Male ◽  
High Fat Diet ◽  
Male Mice ◽  
High Fat

scholarly journals EGCG Prevents High Fat Diet-Induced Changes in Gut Microbiota, Decreases of DNA Strand Breaks, and Changes in Expression and DNA Methylation ofDnmt1andMLH1in C57BL/6J Male Mice

2017 ◽  
Vol 2017 ◽  
pp. 1-17 ◽  
Author(s):  
Marlene Remely ◽  
Franziska Ferk ◽  
Sonja Sterneder ◽  
Tahereh Setayesh ◽  
Sylvia Roth ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Dna Damage ◽  
Gut Microbiota ◽  
High Fat Diet ◽  
Dna Methyltransferase ◽  
Dna Methyltransferase 1 ◽  
Male Mice ◽  
High Fat ◽  
Methyltransferase 1

Obesity as a multifactorial disorder involves low-grade inflammation, increased reactive oxygen species incidence, gut microbiota aberrations, and epigenetic consequences. Thus, prevention and therapies with epigenetic active antioxidants, (-)-Epigallocatechin-3-gallate (EGCG), are of increasing interest. DNA damage, DNA methylation and gene expression ofDNA methyltransferase 1,interleukin 6, andMutL homologue 1were analyzed in C57BL/6J male mice fed a high-fat diet (HFD) or a control diet (CD) with and without EGCG supplementation. Gut microbiota was analyzed with quantitative real-time polymerase chain reaction. An induction of DNA damage was observed, as a consequence of HFD-feeding, whereas EGCG supplementation decreased DNA damage. HFD-feeding induced a higher inflammatory status. Supplementation reversed these effects, resulting in tissue specific gene expression and methylation patterns ofDNA methyltransferase 1andMutL homologue 1. HFD feeding caused a significant lower bacterial abundance. TheFirmicutes/Bacteroidetesratio is significantly lower in HFD + EGCG but higher in CD + EGCG compared to control groups. The results demonstrate the impact of EGCG on the one hand on gut microbiota which together with dietary components affects host health. On the other hand effects may derive from antioxidative activities as well as epigenetic modifications observed on CpG methylation but also likely to include other epigenetic elements.

scholarly journals Gene Expression Profiling in Liver of Mice Fed a High-Fat Diet Supplemented With Watermelon Flesh, Arginine, or Citrulline Shows Similar Pattern Changes

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 303-303
Author(s):  
Mikayla Chen ◽  
Neil Shay
Keyword(s):  
Gene Expression ◽  
High Fat Diet ◽  
Expression Patterns ◽  
Cytochrome P450 Monooxygenases ◽  
Mrna Levels ◽  
Male Mice ◽  
High Fat ◽  
Total Rna ◽  
P450 Monooxygenases ◽  
Close Relationship

Abstract Objectives Watermelon is a nutrient-dense fruit known to contain high levels or arginine and citrulline; these two compounds may influence the nitric oxide pathway, vasodilation, and thus be hypotensive. We tested the hypothesis that when C57BL/6J male mice fed a high-fat diet (HF) had additions to the HF diet of either watermelon flesh (WF), arginine (ARG) or citrulline (CIT), changes in gene expression patterns would occur vs. those seen in HF. Further, we hypothesize that patterns of expression seen in WF, ARG, and CIT groups would be somewhat similar based on increased dietary levels of ARG and CIT in all three groups. Methods Following prior work (Becraft et al.; 2018, Egea et al. 2020), groups of mice were provided either a low-fat diet (LF, 10% kcal fat), high-fat diet (HF, 45% kcal fat), HF plus Watermelon Flesh (WF), HF plus 1% (w/w) arginine (ARG) or 1% (w/w citrulline (CIT) for 10 weeks. Watermelon flesh was provided at 10% of total energy. After ten weeks, animals were euthanized, and liver total RNA was isolated using Trizol. Total RNA was then used for gene expression analysis (N = 4 per group) using Clariom S microarrays and TAC analysis software (ThermoFisher). Results Mice fed WF, ARG, and CIT had several shared canonical pathways of gene expression, including eicosanoid metabolism via cytochrome P450 monooxygenases and exercise-induced circadian rhythm (All P < 0.05). Intake of WF and ARG significantly up-regulated both Cyp2c9 and Cyp2c38 mRNA levels (P < 0.05). The Bst2 gene was significantly down-regulated in all three groups compared to HF mice (P < 0.05). The Cyp2b9 gene was upregulated ∼10.7 fold in WF, and > 1000-fold in ARG mice (P < 0.05). Conclusions We demonstrated that when added to a HF diet, WF, ARG, and CIT all produced a change in hepatic gene expression in male mice. Possibly due to the close relationship of ARG and CIT metabolism, and high content of ARG and CIT in WF, expression patterns observed in all three groups demonstrated a high degree of similarity. Several genes, including Cyp2c9, Cyp2c38, and Elvol5 were up-regulated; these genes may be involved in modifying steroids and arachidonic acid and other long-chain fatty acids. Funding Sources National Watermelon Promotion Board.

scholarly journals Chronic exposure to arsenic and high fat diet additively induced cardiotoxicity in male mice

2018 ◽  
Vol 13 (1) ◽  
pp. 47 ◽  
Author(s):  
Leila Zeidooni ◽  
Akram Ahangarpour ◽  
Azin Samimi ◽  
Soheila Alboghobeish ◽  
LayaSadat Khorsandi ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Chronic Exposure ◽  
Male Mice ◽  
High Fat

scholarly journals Effect of inulin supplementation in male mice fed with high fat diet on biochemical profile and α-amylase gene expression

2016 ◽  
Vol 15 (6) ◽  
pp. 1197 ◽  
Author(s):  
Zahra Pouyamanesh ◽  
Mahsa M Amoli ◽  
Parichehreh Yaghmaei ◽  
Azadeh Ebrahim-Habibi
Keyword(s):  
Gene Expression ◽  
High Fat Diet ◽  
Biochemical Profile ◽  
Male Mice ◽  
High Fat ◽  
Amylase Gene

scholarly journals Hepatic Gene Expression Profiling in Nrf2 Knockout Mice after Long-Term High-Fat Diet-Induced Obesity

2013 ◽  
Vol 2013 ◽  
pp. 1-17 ◽  
Author(s):  
Dionysios V. Chartoumpekis ◽  
Panos G. Ziros ◽  
Apostolos Zaravinos ◽  
Ralitsa P. Iskrenova ◽  
Agathoklis I. Psyrogiannis ◽  
...  
Keyword(s):  
Gene Expression ◽  
Microarray Analysis ◽  
High Fat Diet ◽  
Metabolic Regulation ◽  
Expression Profiles ◽  
Related Factor ◽  
High Fat ◽  
Qrt Pcr ◽  
Nrf2 Knockout

Introduction. The transcription factor NFE2-related factor 2 (Nrf2) is a central regulator of antioxidant and detoxification gene expression in response to electrophilic or oxidative stress. Nrf2 has recently been shown to cross-talk with metabolic pathways, and its gene deletion protected mice from high-fat-diet-(HFD-) induced obesity and insulin resistance. This study aimed to identify potential Nrf2-regulated genes of metabolic interest by comparing gene expression profiles of livers of wild-type (WT) versus Nrf2 knockout (Nrf2-KO) mice after a long-term HFD.Methods. WT and Nrf2-KO mice were fed an HFD for 180 days; total RNA was prepared from liver and used for microarray analysis and quantitative real-time RT-PCR (qRT-PCR).Results. The microarray analysis identified 601 genes that were differentially expressed between WT and Nrf2-KO mice after long-term HFD. Selected genes, including ones known to be involved in metabolic regulation, were prioritized for verification by qRT-PCR:Cyp7a1andFabp5were significantly overexpressed in Nrf2-KO mice; in contrast,Car,Cyp2b10,Lipocalin 13,Aquaporin 8,Cbr3,Me1, andNqo1were significantly underexpressed in Nrf2-KO mice.Conclusion. Transcriptome profiling after HFD-induced obesity confirms thatNrf2is implicated in liver metabolic gene networks. The specific genes identified here may provide insights into Nrf2-dependent mechanisms of metabolic regulation.

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