Identification of Microsatellite Repeats in Turbot (Scophthalmus maximus) and Dover Sole (Solea solea) using a RAPD-Based Technique: Characterization of Microsatellite Markers in Dover Sole

2000 ◽  
Vol 2 (1) ◽  
pp. 49-56 ◽  
Author(s):  
Arati Iyengar ◽  
Sanit Piyapattanakorn ◽  
David M. Stone ◽  
Diana A. Heipel ◽  
Bari R. Howell ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Scophthalmus Maximus ◽  
Microsatellite Repeats ◽  
Solea Solea ◽  
Turbot Scophthalmus Maximus ◽  
Dover Sole

Development and characterization of 248 novel microsatellite markers in turbot (Scophthalmus maximus)

Genome ◽  
2007 ◽  
Vol 50 (3) ◽  
pp. 329-332 ◽  
Author(s):  
B.G. Pardo ◽  
C. Fernández ◽  
M. Hermida ◽  
A. Vázquez-López ◽  
M. Pérez ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Scophthalmus Maximus ◽  
Marine Aquaculture ◽  
Genomic Libraries ◽  
Polymorphic Microsatellites ◽  
Great Relevance ◽  
Trait Locus ◽  
Turbot Scophthalmus Maximus ◽  
Simple Sequence

The turbot is a flatfish species of great relevance to marine aquaculture in Europe. Only a limited number of microsatellites have been isolated to date in this species. To increase the number of potentially useful mapping markers, we screened simple sequence repeat (SSR) - enriched genomic libraries obtained from several di-, tri-, and tetranucleotide tandem repeat motifs. A total of 248 new polymorphic microsatellites were successfully optimized. The efficiency of the protocol applied (6.4%) was higher than that in other studies of fish that used the same method. Dinucleotide and perfect microsatellites were predominant in this species; the (AC)n motif was the most frequent class of repeat. Polymorphism and structural properties at these loci, together with 30 variable loci previously reported in turbot, were evaluated in 6 wild individuals. The number of alleles per locus ranged from 2 to 10, with an average of 4.046. The microsatellite markers characterized in this study will contribute to the development of the turbot genetic map, which can be used for quantitative trait locus (QTL) identification, marker-assisted selection programs, and other applications to improve its culture.

scholarly journals Conservation aspects of natural populations and captive-bred stocks of turbot (Scophthalmus maximus) and Dover sole (Solea solea) using estimates of genetic diversity

2007 ◽  
Vol 64 (6) ◽  
pp. 1173-1181 ◽  
Author(s):  
Athanasios Exadactylos ◽  
Mark J. Rigby ◽  
Audrey J. Geffen ◽  
John P. Thorpe
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Natural Populations ◽  
Genetic Data ◽  
Scophthalmus Maximus ◽  
Effective Population ◽  
Solea Solea ◽  
Population Sizes ◽  
Turbot Scophthalmus Maximus ◽  
Dover Sole

Exadactylos, A., Rigby, M. J., Geffen, A. J., and Thorpe, J. P. 2007. Conservation aspects of natural populations and captive-bred stocks of turbot (Scophthalmus maximus) and Dover sole (Solea solea) using estimates of genetic diversity. – ICES Journal of marine Science, 64: 1173–1181. Population genetic analyses have been highly successful in predicting inter- and intraspecific evolutionary relationships, levels of gene flow, genetic divergence, and effective population sizes. Parameters estimated are evolutionary averages and are therefore relevant for addressing contemporary ecological or conservation issues. Changes in genetic variation within the range of a species may indicate patterns of population structure resulting from past ecological and demographic events that are otherwise difficult to infer, so may provide an insight into evolutionary development. Genetic data, drawn from 14 enzyme loci amplified from two populations of turbot (Scophthalmus maximus) and five populations of Dover sole (Solea solea) from the Irish Sea were used to examine population structure estimated from measures of genetic diversity. The aim was to provide an empirical assessment of whether artificial propagation poses a genetic threat to conservation of naturally spawning populations, and whether the fitness for natural spawning and rearing can be rapidly and substantially reduced or increased by artificial propagation. Because of prolonged overfishing, turbot and sole populations in the region are below natural levels, and survive in small local populations in fragmented habitats. Genetic data derived from allozymes have shown that populations are characterized by relatively low levels of genetic diversity. A hypothetical model supporting genetic population substructure, such as range expansion with founder-flush effects, and subsequent population decline with small effective population sizes was considered. Observations support our belief that conservation measures based on genetic diversity have to be developed to ensure the survival of this diverse gene pool.

Characterization of hepatic flavin monooxygenase from the marine teleost turbot (Scophthalmus maximus L.)

Xenobiotica ◽  
1995 ◽  
Vol 25 (2) ◽  
pp. 121-131 ◽  
Author(s):  
L. D. Peters ◽  
D. R. Livingstone ◽  
S. Shenin-Johnson ◽  
R. N. Hines ◽  
D. Schlenk
Keyword(s):  
Scophthalmus Maximus ◽  
Marine Teleost ◽  
Flavin Monooxygenase ◽  
Turbot Scophthalmus Maximus
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