scholarly journals Optical Biopsy of In Vivo Human Skin: Multi-photon Excitation Microscopy

1998 ◽  
Vol 13 (3) ◽  
pp. 196-203 ◽  
Author(s):  
B.R. Masters ◽  
P.T.C. So ◽  
E. Gratton
Keyword(s):  
Human Skin ◽  
Optical Biopsy ◽  
Photon Excitation

Laser system for optical biopsy and in-vivo study of the human skin

2001 ◽  
Author(s):  
Ekaterina G. Borisova ◽  
Lachezar A. Avramov
Keyword(s):  
Human Skin ◽  
Laser System ◽  
Optical Biopsy ◽  
In Vivo Study

scholarly journals FLAME: Macroscopic imaging with microscopic resolution. Optical biopsy of human skin

2020 ◽  
Author(s):  
Alexander Fast ◽  
Akarsh Lal ◽  
Amanda F. Durkin ◽  
Christopher B. Zachary ◽  
Anand K. Ganesan ◽  
...  
Keyword(s):  
Human Skin ◽  
Single Photon ◽  
Ex Vivo ◽  
Photon Counting ◽  
Optical Biopsy ◽  
Large Area ◽  
Time Resolved ◽  
Distribution Maps ◽  
Imaging Tool

AbstractWe introduce a compact, fast large area multiphoton exoscope (FLAME) system with enhanced molecular contrast for macroscopic imaging of human skin with microscopic resolution. A versatile imaging platform with multiple modes of operation for comprehensive analysis of live or resected thick human skin tissue, it produces 3D images that encompass sub-mm2 to cm2 scale areas of tissue within minutes. The FLAME imaging platform, which expands on a design recently introduced by our group, features deep learning, additional scanning hardware elements and time-resolved single photon counting detection to uniquely allow fast discrimination and 3D virtual staining of melanin. We demonstrate its performance and utility by fast ex vivo and in vivo imaging of human skin. With the ability to provide rapid access to depth resolved images of skin over cm2 area and to generate 3D distribution maps of key sub-cellular skin components such as melanocytic dendrites and melanin, FLAME represents a promising imaging tool for enhancing diagnosis accuracy, guiding therapy and understanding skin biology.

Multi-photon Excitation Microscopy and Confocal Microscopy Imaging of In Vivo Human Skin: A Comparison

1999 ◽  
Vol 5 (4) ◽  
pp. 282-289 ◽  
Author(s):  
Barry R. Masters ◽  
Peter T.C. So
Keyword(s):  
Confocal Microscopy ◽  
Human Skin ◽  
Structural Information ◽  
Laser Pulses ◽  
Skin Surface ◽  
Femtosecond Laser Pulses ◽  
Microscopy Imaging ◽  
Photon Excitation ◽  
Reflected Light

Abstract: We compare here multi-photon excitation microscopy and tandem scanning reflected light confocal microscopy for the microscopic observation of human skin in vivo. Multi-photon excitation is induced by a 80-MHz pulse train of femtosecond laser pulses at 780 nm wavelength. This nonlinear microscopic technique is inherently suitable for tissue fluorescence imaging because of its deeper penetration depth and lower specimen photodamage. This technique has noninvasively obtained tissue structural information in human epidermis and dermis. Alternatively, tandem scanning confocal light microscopy based on a white light source can provide video-rate image acquisition with high resolution and high contrast. Reflected light confocal methods have been used to obtain images from the skin surface to the epidermal–dermal junction. The relative merits of these two techniques can be identified by comparing three-dimensionally resolved images obtained from the forearm skin of the same volunteer.

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