Diagnostic Value of Cytokine Assays in Cerebrospinal Fluid in Culture-Negative, Polymerase Chain Reaction-Positive Bacterial Meningitis

2000 ◽  
Vol 19 (5) ◽  
pp. 388-392 ◽  
Author(s):  
G. Lorino ◽  
S. Angeletti ◽  
G. Gherardi ◽  
M. De Cesaris ◽  
L. Gherardi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cerebrospinal Fluid ◽  
Bacterial Meningitis ◽  
Diagnostic Value ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Culture Negative ◽  
Negative Polymerase Chain Reaction

scholarly journals Detection of Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae in Culture Negative Cerebrospinal Fluid Samples from Meningitis Patients Using a Multiplex Polymerase Chain Reaction in Nepal

2021 ◽  
Vol 13 (1) ◽  
pp. 173-180
Author(s):  
Supriya Sharma ◽  
Jyoti Acharya ◽  
Dominique A. Caugant ◽  
Megha Raj Banjara ◽  
Prakash Ghimire ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cerebrospinal Fluid ◽  
Streptococcus Pneumoniae ◽  
Bacterial Meningitis ◽  
Haemophilus Influenzae ◽  
Neisseria Meningitidis ◽  
Multiplex Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Culture Negative

The rapid identification of bacteria causing meningitis is crucial as delays in the treatment increase mortality rate. Though considered as the gold standard for the laboratory diagnosis of bacterial meningitis, culture might give false negative results in a case of patients under antibiotics prior to lumbar puncture. This study aimed to detect Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae by a multiplex polymerase chain reaction (PCR) in culture-negative cerebrospinal fluid samples collected from clinically suspected meningitis cases attending different hospitals in Kathmandu, Nepal from January 2017 to December 2019. S. pneumoniae, N. meningitidis and H. influenzae were detected in 8.59% (33/384) of the specimens by PCR and 7.55% (29/384) of the specimens by culture. Correlation between culture and PCR of the same sample was good (Spearman’s rho correlation coefficient = 0.932). However, the difference in positivity between culture and PCR was statistically not significant (p value > 0.05). In four specimens, culture could not detect any of the targeted bacteria whereas PCR could detect presence of H. influenzae. PCR increases the diagnostic yield for bacterial meningitis. PCR may be considered as an adjunctive test for establishing the cause of infection in culture negative clinically suspected meningitis cases.

scholarly journals Polymerase chain reaction, with sequencing, as a diagnostic tool in culture-negative bacterial meningitis

1999 ◽  
Vol 5 (2) ◽  
pp. 92-96 ◽  
Author(s):  
Giordano Dicuonzo ◽  
Giulia Lorino ◽  
Daniela Lilli ◽  
Daniela Rivanera ◽  
Paola Guarino ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Bacterial Meningitis ◽  
Diagnostic Tool ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Culture Negative

scholarly journals Culture NegativeListeria monocytogenesMeningitis Resulting in Hydrocephalus and Severe Neurological Sequelae in a Previously Healthy Immunocompetent Man with Penicillin Allergy

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Shahin Gaini ◽  
Gunn Hege Karlsen ◽  
Anirban Nandy ◽  
Heidi Madsen ◽  
Debes Hammershaimb Christiansen ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cerebrospinal Fluid ◽  
Listeria Monocytogenes ◽  
Bacterial Meningitis ◽  
Penicillin Allergy ◽  
Ventricular Catheter ◽  
Chain Reaction ◽  
Neck Stiffness ◽  
Neurological Sequelae ◽  
Polymerase Chain

A previously healthy 74-year-old Caucasian man with penicillin allergy was admitted with evolving headache, confusion, fever, and neck stiffness. Treatment for bacterial meningitis with dexamethasone and monotherapy ceftriaxone was started. The cerebrospinal fluid showed negative microscopy for bacteria, no bacterial growth, and negative polymerase chain reaction for bacterial DNA. The patient developed hydrocephalus on a second CT scan of the brain on the 5th day of admission. An external ventricular catheter was inserted andListeria monocytogenesgrew in the cerebrospinal fluid from the catheter. The patient had severe neurological sequelae. This case report emphasises the importance of covering empirically forListeria monocytogenesin all patients with penicillin allergy with suspected bacterial meningitis. The case also shows that it is possible to have significant infection and inflammation even with negative microscopy, negative cultures, and negative broad range polymerase chain reaction in cases ofListeriameningitis. Follow-up spinal taps can be necessary to detect the presence ofListeria monocytogenes.

scholarly journals Incidence of bacterial meningitis in Asia using enhanced CSF testing: polymerase chain reaction, latex agglutination and culture

2007 ◽  
Vol 135 (7) ◽  
pp. 1217-1226 ◽  
Author(s):  
W. A. KENNEDY ◽  
S-J. CHANG ◽  
K. PURDY ◽  
T. LE ◽  
P. E. KILGORE ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cerebrospinal Fluid ◽  
Bacterial Meningitis ◽  
Bacterial Culture ◽  
East Asian ◽  
Latex Agglutination ◽  
Type B ◽  
Chain Reaction ◽  
Surveillance Studies ◽  
Polymerase Chain

SUMMARYTo enhance the detection of bacterial meningitis in an East Asian surveillance study, we employed cerebrospinal fluid (CSF) bacterial culture, latex agglutination (LA) and polymerase chain reaction–enzyme immunoassay (PCR–EIA) testing forHaemophilus influenzaetype b (Hib) andStreptococcus pneumoniae(Sp). The sensitivity and specificity of CSF PCR–EIA testing was compared to LA and culture. A meningitis case was defined by one positive result for any of the three tests. The sensitivity ofH. influenzaeCSF PCR–EIA, LA, and culture was 100%, 40% and 57·5% respectively; and for Sp CSF PCR–EIA, LA and culture, the sensitivity was 100%, 58·3% and 66·7%, respectively. Hib and Sp specificity was 100% by each method. CSF PCR–EIA was more sensitive than culture or LA for the detection of Hib and Sp meningitis cases increasing their incidence by 74% and 50% compared to culture respectively. CSF PCR–EIA should be included for the detection of bacterial meningitis in surveillance studies.

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