Comparative Activity of Moxifloxacin in Vitro Against Obligately Anaerobic Bacteria

2000 ◽  
Vol 19 (3) ◽  
pp. 228-232 ◽  
Author(s):  
G. Ackermann ◽  
R. Schaumann ◽  
B. Pless ◽  
M. C. Claros ◽  
E. J. C. Goldstein ◽  
...  
Keyword(s):  
Anaerobic Bacteria ◽  
Obligately Anaerobic ◽  
Comparative Activity

scholarly journals In Vitro Activity of OPT-80 against Clostridium difficile

2004 ◽  
Vol 48 (6) ◽  
pp. 2280-2282 ◽  
Author(s):  
Grit Ackermann ◽  
Birgit Löffler ◽  
Daniela Adler ◽  
Arne C. Rodloff
Keyword(s):  
Clostridium Difficile ◽  
Anaerobic Bacteria ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Obligately Anaerobic ◽  
Nosocomial Diarrhea ◽  
Frequent Relapses

ABSTRACT Clostridium difficile remains the major cause of nosocomial diarrhea. Reports on impaired susceptibility of C. difficile to metronidazole and vancomycin and frequent relapses of patients after therapy necessitate the search for new substances. With this study, the activity of OPT-80, a new macrocycle, against 207 C. difficile strains and against other obligately anaerobic bacteria was tested. OPT-80 showed high in vitro activity against all C. difficile strains tested.

scholarly journals Comparative In Vitro Activities of Gemifloxacin, Other Quinolones, and Nonquinolone Antimicrobials against Obligately Anaerobic Bacteria

2001 ◽  
Vol 45 (6) ◽  
pp. 1896-1899 ◽  
Author(s):  
Niels Kleinkauf ◽  
Grit Ackermann ◽  
Reiner Schaumann ◽  
Arne C. Rodloff
Keyword(s):  
Anaerobic Bacteria ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Dilution Technique ◽  
Gram Negative ◽  
Obligately Anaerobic ◽  
Agar Dilution

ABSTRACT The in vitro activity of gemifloxacin was compared to that of other quinolone and nonquinolone antimicrobials against 204 anaerobes by the agar dilution technique. The data indicate that gemifloxacin has a rather selective anaerobic activity. Most Peptostreptococcus, Porphyromonas, and Fusobacterium species are susceptible, while gemifloxacin's activity against other gram-negative anaerobes appears to be variable.

In vitro activities of fourteen antimicrobial agents against obligately anaerobic bacteria

2000 ◽  
Vol 16 (3) ◽  
pp. 225-232 ◽  
Author(s):  
Reiner Schaumann ◽  
Grit Ackermann ◽  
Baerbel Pless ◽  
Marina C Claros ◽  
Ellie J.C Goldstein ◽  
...  
Keyword(s):  
Antimicrobial Agents ◽  
Anaerobic Bacteria ◽  
Obligately Anaerobic

scholarly journals In Vitro Activities of Gatifloxacin, Two Other Quinolones, and Five Nonquinolone Antimicrobials against Obligately Anaerobic Bacteria

1999 ◽  
Vol 43 (11) ◽  
pp. 2783-2786 ◽  
Author(s):  
Reiner Schaumann ◽  
Grit Ackermann ◽  
Baerbel Pless ◽  
Marina C. Claros ◽  
Arne C. Rodloff
Keyword(s):  
Antimicrobial Agents ◽  
Anaerobic Bacteria ◽  
Broth Microdilution ◽  
Obligately Anaerobic

ABSTRACT The activity of the new fluoroquinolone gatifloxacin was compared with those of other quinolones and antimicrobial agents of other classes against 294 anaerobes by the broth microdilution technique. For all strains tested, gatifloxacin MICs at which 50 and 90% of the isolates were inhibited were 0.5 and 2 mg/liter, respectively, and were 3 to 4 dilution steps lower than, e.g., ciprofloxacin.

In Vitro Activity of New Quinolones against Anaerobic Bacteria

Drugs ◽  
1995 ◽  
Vol 49 (Supplement 2) ◽  
pp. 233-234
Author(s):  
Carl Erik Nord ◽  
Ann Lindmark ◽  
Ingela Persson
Keyword(s):  
Anaerobic Bacteria ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
New Quinolones

scholarly journals The in-vitro activity of trovafloxacin and nine other antimicrobials against 413 anaerobic bacteria

1996 ◽  
Vol 38 (2) ◽  
pp. 271-281 ◽  
Author(s):  
K. E. Bowker ◽  
M. Wootton ◽  
H. A. Holt ◽  
D. S. Reeves ◽  
A. P. MacGowan
Keyword(s):  
Anaerobic Bacteria ◽  
Vitro Activity ◽  
In Vitro Activity

Comparative activity of anticestode drugs—praziquantel, niclosamide and Compound 77–6, against Hymenolepis nana

1983 ◽  
Vol 57 (1) ◽  
pp. 31-36 ◽  
Author(s):  
Suman Gupta ◽  
J. C. Katiyar
Keyword(s):  
Drug Concentration ◽  
Hymenolepis Nana ◽  
Comparative Activity

AbstractThe activity, in terms of speed of action, of three anticestode drugs against Hymenolepis nana, both in vivo and in vitro, was investigated. Praziquantel was most effective in vivo, but had little action on adult worms and cysticercoids in vitro. Niclosamide, the least effective in vivo, was highly toxic in vitro. Compound 77–6 killed adult worms and cysticercoids in vitro in 10 min and 15 min respectively at 1000 μg/ml of drug concentration, but its in viro effect was intermediate between that of praziquantel and niclosamide.

scholarly journals In Vitro Reconstitution of an NADPH-Dependent Superoxide Reduction Pathway from Pyrococcus furiosus

2005 ◽  
Vol 71 (3) ◽  
pp. 1522-1530 ◽  
Author(s):  
Amy M. Grunden ◽  
Francis E. Jenney ◽  
Kesen Ma ◽  
Mikyoung Ji ◽  
Michael V. Weinberg ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Pyrococcus Furiosus ◽  
Expression System ◽  
Anaerobic Bacteria ◽  
Flavin Mononucleotide ◽  
Superoxide Reductase ◽  
Recombinant Enzymes ◽  
Significant Similarity ◽  
Midpoint Potential

ABSTRACT A scheme for the detoxification of superoxide in Pyrococcus furiosus has been previously proposed in which superoxide reductase (SOR) reduces (rather than dismutates) superoxide to hydrogen peroxide by using electrons from reduced rubredoxin (Rd). Rd is reduced with electrons from NAD(P)H by the enzyme NAD(P)H:rubredoxin oxidoreductase (NROR). The goal of the present work was to reconstitute this pathway in vitro using recombinant enzymes. While recombinant forms of SOR and Rd are available, the gene encoding P. furiosus NROR (PF1197) was found to be exceedingly toxic to Escherichia coli, and an active recombinant form (rNROR) was obtained via a fusion protein expression system, which produced an inactive form of NROR until cleavage. This allowed the complete pathway from NAD(P)H to the reduction of SOR via NROR and Rd to be reconstituted in vitro using recombinant proteins. rNROR is a 39.9-kDa protein whose sequence contains both flavin adenine dinucleotide (FAD)- and NAD(P)H-binding motifs, and it shares significant similarity with known and putative Rd-dependent oxidoreductases from several anaerobic bacteria, both mesophilic and hyperthermophilic. FAD was shown to be essential for activity in reconstitution assays and could not be replaced by flavin mononucleotide (FMN). The bound FAD has a midpoint potential of −173 mV at 23°C (−193 mV at 80°C). Like native NROR, the recombinant enzyme catalyzed the NADPH-dependent reduction of rubredoxin both at high (80°C) and low (23°C) temperatures, consistent with its proposed role in the superoxide reduction pathway. This is the first demonstration of in vitro superoxide reduction to hydrogen peroxide using NAD(P)H as the electron donor in an SOR-mediated pathway.

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