Formation and manipulation of regular metallic nanoparticle arrays on bacterial surface layers: an advanced TEM study

2001 ◽  
Vol 16 (1) ◽  
pp. 317-320 ◽  
Author(s):  
M. Mertig ◽  
R. Wahl ◽  
M. Lehmann ◽  
P. Simon ◽  
W. Pompe
1990 ◽  
Vol 36 (5) ◽  
pp. 327-335 ◽  
Author(s):  
Stephen H. Smith ◽  
Robert G. E. Murray

Aquaspirillum sinuosum cell walls bear two paracrystalline, proteinaceous surface layers (S layers). Each shows a different symmetry: the inner layer is closely apposed to the outer membrane and is a tetragonal array (90° axes; 5-nm units; repeat frequency 8 nm); the outer layer is a hexagonal array on the external surface (14-nm units; repeat frequency 18 nm) and, although the units have a six-pointed stellate form, the linkage between units is not resolved. The outer layer consists of a major 130-kDa protein and a 180-kDa minor component; these co-extract, co-assemble, and are inseparable by hydroxylapatite chromatography or by recrystallization. The solubilizing effects of reagents suggest stabilization by hydrogen bonding and Ca2+. The two outer layer proteins are serologically related and show partial identity by peptide mapping. Periodic acid – Schiff staining of the 180-kDa band suggests that this may be a glycosylated form of the 130-kDa component. The inner layer components form a doublet of 75- and 80-kDa polypeptides with extreme resistance to extraction. Close apposition to the outer membrane, resistance to chaotropes, aqueous insolubility, and behaviour in charge-shift electrophoresis suggest hydrophobic interaction between subunits and an integral association with the outer membrane. Key words: bacterial surface, cell wall, surface layers, cell-wall proteins, cell-wall assembly.


2007 ◽  
Vol 101 (5) ◽  
pp. 054304 ◽  
Author(s):  
Elefterios Lidorikis ◽  
Shunji Egusa ◽  
J. D. Joannopoulos

2014 ◽  
Vol 118 (33) ◽  
pp. 19315-19321 ◽  
Author(s):  
Ying Bao ◽  
Zijie Yan ◽  
Norbert F. Scherer

2011 ◽  
Author(s):  
Tino Benkenstein ◽  
Michael Flämmich ◽  
Torsten Harzendorf ◽  
Thomas Käsebier ◽  
Dirk Michaelis ◽  
...  

Author(s):  
Dietmar Pum ◽  
Claudia Hödl ◽  
Ursula Kainz ◽  
Angela Neubauer ◽  
Walter Schreier ◽  
...  

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