Aqueous mixtures of spherical and rodlike (flexible) macroparticles: structure and dynamics of latex spheres in saltfree solutions

1999 ◽  
Vol 10 (3) ◽  
pp. 525-536 ◽  
Author(s):  
J. Biegel ◽  
C. Hundegger ◽  
B. Nadig ◽  
R. Weber
Author(s):  
Neda Beheshti ◽  
Giao T.M. Nguyen ◽  
Anna-Lena Kjøniksen ◽  
Kenneth D. Knudsen ◽  
Bo Nyström

Author(s):  
Alain R. Trudel ◽  
M. Trudel

AirfugeR (Beckman) direct ultracentrifugation of viral samples on electron microscopy grids offers a rapid way to concentrate viral particles or subunits and facilitate their detection and study. Using the A-100 fixed angle rotor (30°) with a K factor of 19 at maximum speed (95 000 rpm), samples up to 240 μl can be prepared for electron microscopy observation in a few minutes: observation time is decreased and structural details are highlighted. Using latex spheres to calculate the increase in sensitivity compared to the inverted drop procedure, we obtained a 10 to 40 fold increase in sensitivity depending on the size of particles. This technique also permits quantification of viral particles in samples if an aliquot is mixed with latex spheres of known concentration.Direct ultracentrifugation for electron microscopy can be performed on laboratory samples such as gradient or column fractions, infected cell supernatant, or on clinical samples such as urine, tears, cephalo-rachidian liquid, etc..


2006 ◽  
Vol 73 ◽  
pp. 109-119 ◽  
Author(s):  
Chris Stockdale ◽  
Michael Bruno ◽  
Helder Ferreira ◽  
Elisa Garcia-Wilson ◽  
Nicola Wiechens ◽  
...  

In the 30 years since the discovery of the nucleosome, our picture of it has come into sharp focus. The recent high-resolution structures have provided a wealth of insight into the function of the nucleosome, but they are inherently static. Our current knowledge of how nucleosomes can be reconfigured dynamically is at a much earlier stage. Here, recent advances in the understanding of chromatin structure and dynamics are highlighted. The ways in which different modes of nucleosome reconfiguration are likely to influence each other are discussed, and some of the factors likely to regulate the dynamic properties of nucleosomes are considered.


1998 ◽  
Vol 77 (2) ◽  
pp. 357-362 ◽  
Author(s):  
A. Matic, L. Borjesson

Author(s):  
V. D. Tereshchenko ◽  
E. B. Vasil'ev ◽  
O. F. Ogloblina ◽  
V. A. Tereshchenko ◽  
S. M. Chernyakov

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