Electronic identification and chromosomal assignment by radiation hybrid mapping of human expressed sequence tags corresponding to new potassium channel genes

Neurogenetics ◽  
1999 ◽  
Vol 2 (2) ◽  
pp. 115-120
Author(s):  
Pierre Szepetowski ◽  
Anthony P. Monaco
Keyword(s):  
Potassium Channel ◽  
Radiation Hybrid ◽  
Expressed Sequence Tags ◽  
Radiation Hybrid Mapping ◽  
Chromosomal Assignment ◽  
Hybrid Mapping ◽  
Expressed Sequence

Characterization and radiation hybrid mapping of expressed sequence tags from the canine brain

2003 ◽  
Vol 14 (3) ◽  
pp. 203-213 ◽  
Author(s):  
Monica C. Roberts ◽  
Christophe Hitte ◽  
Julie A. Hendrickson ◽  
Daniel E. Hoffmann ◽  
Gail H. Flickinger ◽  
...  
Keyword(s):  
Radiation Hybrid ◽  
Expressed Sequence Tags ◽  
Radiation Hybrid Mapping ◽  
Hybrid Mapping ◽  
Canine Brain ◽  
Expressed Sequence

scholarly journals Generation and chromosome mapping of expressed sequence tags (ESTs) from a human infant thymus

Genome ◽  
1999 ◽  
Vol 42 (3) ◽  
pp. 457-464 ◽  
Author(s):  
Mee-Yul Hwang ◽  
Yoon-Joong Kang ◽  
Young-Ho Kim ◽  
Steve W Scherer ◽  
Lap-Chee Tsui ◽  
...  
Keyword(s):  
Expression Profile ◽  
Radiation Hybrid ◽  
Expressed Sequence Tags ◽  
Radiation Hybrid Mapping ◽  
Human Infant ◽  
Hybrid Mapping ◽  
Novel Genes ◽  
Ehlers Danlos Syndrome ◽  
Disease Loci ◽  
Expressed Sequence

In an effort to identify novel genes that are expressed differentially in an infant thymus, we constructed an oligo-d(T) primed cDNA library from a human infant thymus followed by single-run partial sequencing to generate expressed sequence tags (ESTs). Characterization of more than 1400 sequences enabled us to convert human thymus transcripts into 1223 useful ESTs. These ESTs consisted of 613 (50.1%) showing homology to known human genes, 51 (4.2%) matching to genes from other species, 289 (23.6%) matching ESTs of unknown functions, and 182 (14.9%) being novel transcripts. The expression profile of an infant thymus features a high number of genes related to cell division-DNA synthesis and gene-protein expression, indicating the active growth stage of an infant thymus. To identify the chromosomal localization of 43 thymus ESTs, PCR-based mapping was performed using a human-rodent somatic cell hybrid or radiation hybrid mapping panel. The results indicated that several novel genes were determined to be located in the vicinity of previously mapped disease loci; histidinemia loci, plasminogen Tochigi disease loci, Ehlers-Danlos syndrome, hypertriglyceridemia, thyroid resistance locus, ocular albinism, galactosemia, porphyria variegata, Charcot-Marie-tooth disease, FEOM (fibrosis of extraocular muscles), Prader-Willi syndrome.Key words: cDNA, expression profile, radiation hybrid mapping, disease locus.

Chromosomal assignment of loci susceptible to replication errors by radiation hybrid mapping

1998 ◽  
Vol 382 (3-4) ◽  
pp. 81-83
Author(s):  
Maja Krajinovic ◽  
Chantal Richer ◽  
Ljiljana Lukovic ◽  
Damian Labuda ◽  
Daniel Sinnett
Keyword(s):  
Radiation Hybrid ◽  
Radiation Hybrid Mapping ◽  
Chromosomal Assignment ◽  
Hybrid Mapping ◽  
Replication Errors
Sign in / Sign up

Export Citation Format

Share Document