Ultrastructure of Kaposi's sarcoma-associated herpesvirus (KSHV)/human herpesvirus-8 (HHV-8) in a primary effusion lymphoma cell line treated with tetradecanoyl phorbol acetate (TPA)

1999 ◽  
Vol 32 (2) ◽  
pp. 94-99 ◽  
Author(s):  
Yuji Ohtsuki ◽  
Jun Iwata ◽  
Mutsuo Furihata ◽  
Tamotsu Takeuchi ◽  
Hiroshi Sonobe ◽  
...  
Keyword(s):  
Cell Line ◽  
Kaposi's Sarcoma ◽  
Primary Effusion Lymphoma ◽  
Human Herpesvirus ◽  
Lymphoma Cell ◽  
Human Herpesvirus 8 ◽  
Lymphoma Cell Line ◽  
Herpesvirus 8 ◽  
Primary Effusion Lymphoma Cell ◽  
Tetradecanoyl Phorbol Acetate

scholarly journals Human Herpesvirus 8 Gene Encodes a Functional Thymidylate Synthase

2002 ◽  
Vol 76 (20) ◽  
pp. 10530-10532 ◽  
Author(s):  
Gábor Gáspár ◽  
Erik De Clercq ◽  
Johan Neyts
Keyword(s):  
Cell Line ◽  
Thymidylate Synthase ◽  
Kaposi's Sarcoma ◽  
Human Herpesvirus ◽  
Kaposi’S Sarcoma ◽  
Lymphoma Cell ◽  
Human Herpesvirus 8 ◽  
Lymphoma Cell Line ◽  
Herpesvirus 8

ABSTRACT We demonstrate that human herpesvirus 8, obtained from the lymphoma cell line BC-3 as well as from Kaposi's sarcoma lesions, carries a gene that encodes a functional thymidylate synthase (TS). The particular characteristics of this enzyme are studied and compared to the characteristics of TSs encoded by other organisms.

Human interleukin-6 induces human herpesvirus-8 replication in a body cavity-based lymphoma cell line

2002 ◽  
Vol 68 (3) ◽  
pp. 404-411 ◽  
Author(s):  
Jian Song ◽  
Takako Ohkura ◽  
Masamichi Sugimoto ◽  
Yasuko Mori ◽  
Reiko Inagi ◽  
...  
Keyword(s):  
Cell Line ◽  
Interleukin 6 ◽  
Human Herpesvirus ◽  
Body Cavity ◽  
Lymphoma Cell ◽  
Human Herpesvirus 8 ◽  
Lymphoma Cell Line ◽  
Herpesvirus 8

scholarly journals Differential Expression of Viral Bcl-2 Encoded by Kaposi's Sarcoma-Associated Herpesvirus and Human Bcl-2 in Primary Effusion Lymphoma Cells and Kaposi's Sarcoma Lesions

2002 ◽  
Vol 76 (5) ◽  
pp. 2551-2556 ◽  
Author(s):  
Isabelle Widmer ◽  
Marion Wernli ◽  
Felix Bachmann ◽  
Fred Gudat ◽  
Gieri Cathomas ◽  
...  
Keyword(s):  
Kaposi's Sarcoma ◽  
Mononuclear Cells ◽  
Primary Effusion Lymphoma ◽  
Human Herpesvirus ◽  
Kaposi’S Sarcoma ◽  
Human Herpesvirus 8 ◽  
Lymphoma Cells ◽  
Herpesvirus 8 ◽  
Primary Effusion Lymphoma Cell ◽  
Spindle Cells

ABSTRACT Expression of human herpesvirus 8 viral Bcl-2 protein was demonstrated in spindle cells of late-stage Kaposi's sarcoma lesions but not in primary effusion lymphoma cell lines. In contrast, strong expression of human Bcl-2 was found in stimulated primary effusion lymphoma cells, whereas in Kaposi's sarcoma lesions preferential mononuclear cells, and to a lesser extent spindle cells, stained positive.

scholarly journals Human Immunodeficiency Virus Replication in a Primary Effusion Lymphoma Cell Line Stimulates Lytic-Phase Replication of Kaposi’s Sarcoma-Associated Herpesvirus

1999 ◽  
Vol 73 (12) ◽  
pp. 10329-10338 ◽  
Author(s):  
Vasundhara Varthakavi ◽  
Philip J. Browning ◽  
Paul Spearman
Keyword(s):  
Human Immunodeficiency Virus ◽  
Cell Line ◽  
Kaposi's Sarcoma ◽  
Primary Effusion Lymphoma ◽  
Kaposi’S Sarcoma ◽  
Lytic Replication ◽  
Lymphoma Cell ◽  
Lymphoma Cell Line ◽  
Primary Effusion Lymphoma Cell ◽  
Immunodeficiency Virus

ABSTRACT Human immunodeficiency virus (HIV) and Kaposi’s sarcoma-associated herpesvirus (KSHV) coinfect many individuals in North America and in parts of Africa. Infection with HIV is a leading risk factor for the development of Kaposi’s sarcoma (KS). In this study, we tested the hypothesis that HIV infection of common or adjacent cells would stimulate replication and spread of KSHV. Infection of a primary effusion lymphoma cell line by vesicular stomatitis virus type G-pseudotyped HIV type 1 led to a rapid induction of lytic-phase KSHV replication. Induction of lytic KSHV replication by HIV required active replication of HIV. The addition of the nucleoside reverse transcriptase inhibitor azidothymidine or the protease inhibitor indinavir to the culture prevented HIV spread and inhibited the associated induction of KSHV lytic replication. Lytic replication occurred in both HIV-infected and HIV-uninfected cells within the culture, and could be induced in uninfected cells via a soluble factor released from the HIV-infected cells. Transmission of infectious KSHV to an uninfected target cell was enhanced by HIV replication and was inhibited by antiretroviral drugs. These results may have implications for the pathogenesis and treatment of KS in individuals coinfected with KSHV and HIV.

Establishment of a Primary Effusion Lymphoma Cell Line (RM-P1) and In Vivo Growth System Using SCID Mice

2002 ◽  
Vol 76 (2) ◽  
pp. 165-172 ◽  
Author(s):  
Jun-ichi Miyagi ◽  
Masato Masuda ◽  
Nobuyuki Takasu ◽  
Akitoshi Nagasaki ◽  
Tetsuharu Shinjyo ◽  
...  
Keyword(s):  
Cell Line ◽  
Primary Effusion Lymphoma ◽  
Scid Mice ◽  
Lymphoma Cell ◽  
Lymphoma Cell Line ◽  
Primary Effusion Lymphoma Cell ◽  
Growth System ◽  
In Vivo Growth
Sign in / Sign up

Export Citation Format

Share Document