ATP generation during reduced inorganic sulfur compound oxidation by Acidithiobacillus caldus is exclusively due to electron transport phosphorylation

Extremophiles ◽  
2002 ◽  
Vol 6 (2) ◽  
pp. 123-129 ◽  
Author(s):  
Kevin Hallberg ◽  
Mark Dopson ◽  
Börje Lindström
Keyword(s):  
Electron Transport ◽  
Sulfur Compound ◽  
Inorganic Sulfur ◽  
Acidithiobacillus Caldus ◽  
Atp Generation ◽  
Inorganic Sulfur Compound

Utilisation of Inorganic Substrates by Alicyclobacillus - Like Strain FP1

2015 ◽  
Vol 1130 ◽  
pp. 308-311 ◽  
Author(s):  
Helen R. Watling ◽  
David M. Collinson ◽  
Felicity A. Perrot ◽  
Denis W. Shiers
Keyword(s):  
Growth Factor ◽  
Yeast Extract ◽  
Elemental Sulfur ◽  
Sulfur Compound ◽  
Growth Media ◽  
Inorganic Sulfur ◽  
Inorganic Substrates ◽  
Water Ph ◽  
Abiotic Controls ◽  
Inorganic Sulfur Compound

As Alicyclobacillus-like strain FP1 was isolated from copper heap process water (pH 1.5), this research was directed towards its bioleaching attributes, specifically ferrous ion (Fe (II)) and reduced inorganic sulfur compound (RISC) oxidation, and bioleaching of sulfide minerals. Strain FP1 oxidised iron (II) but not tetrathionate or elemental sulfur in growth media containing yeast extract as growth factor. The addition of tetrathionate (2.5 mM) suppressed iron (II) oxidation. Strain FP1 grew on pyrite, arsenopyrite, chalcopyrite, sphalerite and pentlandite in BSM-YE medium at 30 °C and pH 1.8 (35 days), enhancing Zn, Co (in cobaltiferous pyrite), Fe and As recovery, but not Cu or Ni, relative to abiotic controls.

scholarly journals Electricity generation from an inorganic sulfur compound containing mining wastewater by acidophilic microorganisms

2016 ◽  
Vol 167 (7) ◽  
pp. 568-575 ◽  
Author(s):  
Gaofeng Ni ◽  
Stephan Christel ◽  
Pawel Roman ◽  
Zhen Lim Wong ◽  
Martijn F.M. Bijmans ◽  
...  
Keyword(s):  
Electricity Generation ◽  
Sulfur Compound ◽  
Acidophilic Microorganisms ◽  
Inorganic Sulfur ◽  
Mining Wastewater ◽  
Inorganic Sulfur Compound

scholarly journals RNA transcript sequencing reveals inorganic sulfur compound oxidation pathways in the acidophileAcidithiobacillus ferrivorans

2016 ◽  
Vol 363 (7) ◽  
pp. fnw057 ◽  
Author(s):  
Stephan Christel ◽  
Jimmy Fridlund ◽  
Antoine Buetti-Dinh ◽  
Moritz Buck ◽  
Elizabeth L. Watkin ◽  
...  
Keyword(s):  
Sulfur Compound ◽  
Inorganic Sulfur ◽  
Rna Transcript ◽  
Inorganic Sulfur Compound

scholarly journals Gene Identification and Substrate Regulation Provide Insights into Sulfur Accumulation during Bioleaching with the Psychrotolerant Acidophile Acidithiobacillus ferrivorans

2012 ◽  
Vol 79 (3) ◽  
pp. 951-957 ◽  
Author(s):  
Maria Liljeqvist ◽  
Olena I. Rzhepishevska ◽  
Mark Dopson
Keyword(s):  
Acidithiobacillus Ferrooxidans ◽  
Ferrous Iron ◽  
Sulfur Compound ◽  
Iron Oxidation ◽  
Sulfur Compounds ◽  
Content Type ◽  
Inorganic Sulfur ◽  
Cold Environments ◽  
Ferrous Iron Oxidation ◽  
Inorganic Sulfur Compound

ABSTRACTThe psychrotolerant acidophileAcidithiobacillus ferrivoranshas been identified from cold environments and has been shown to use ferrous iron and inorganic sulfur compounds as its energy sources. A bioinformatic evaluation presented in this study suggested thatAcidithiobacillus ferrivoransutilized a ferrous iron oxidation pathway similar to that of the related speciesAcidithiobacillus ferrooxidans. However, the inorganic sulfur oxidation pathway was less clear, since theAcidithiobacillus ferrivoransgenome contained genes from bothAcidithiobacillus ferrooxidansandAcidithiobacillus caldusencoding enzymes whose assigned functions are redundant. Transcriptional analysis revealed that thepetA1andpetB1genes (implicated in ferrous iron oxidation) were downregulated upon growth on the inorganic sulfur compound tetrathionate but were on average 10.5-fold upregulated in the presence of ferrous iron. In contrast, expression ofcyoB1(involved in inorganic sulfur compound oxidation) was decreased 6.6-fold upon growth on ferrous iron alone. Competition assays between ferrous iron and tetrathionate withAcidithiobacillus ferrivoransSS3 precultured on chalcopyrite mineral showed a preference for ferrous iron oxidation over tetrathionate oxidation. Also, pure and mixed cultures of psychrotolerant acidophiles were utilized for the bioleaching of metal sulfide minerals in stirred tank reactors at 5 and 25°C in order to investigate the fate of ferrous iron and inorganic sulfur compounds. Solid sulfur accumulated in bioleaching cultures growing on a chalcopyrite concentrate. Sulfur accumulation halted mineral solubilization, but sulfur was oxidized after metal release had ceased. The data indicated that ferrous iron was preferentially oxidized during growth on chalcopyrite, a finding with important implications for biomining in cold environments.

scholarly journals Genomic Analysis Unravels Reduced Inorganic Sulfur Compound Oxidation of Heterotrophic AcidophilicAcidicaldussp. Strain DX-1

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Yuanyuan Liu ◽  
Hongying Yang ◽  
Xian Zhang ◽  
Yunhua Xiao ◽  
Xue Guo ◽  
...  
Keyword(s):  
Sulfur Compound ◽  
Genomic Analysis ◽  
Direct Oxidation ◽  
Quinone Oxidoreductase ◽  
Heterodisulfide Reductase ◽  
Significant Similarity ◽  
Inorganic Sulfur ◽  
Genes Encoding ◽  
Sulfur Oxidizing ◽  
Inorganic Sulfur Compound

Although reduced inorganic sulfur compound (RISC) oxidation in many chemolithoautotrophic sulfur oxidizers has been investigated in recent years, there is little information about RISC oxidation in heterotrophic acidophiles. In this study,Acidicaldussp. strain DX-1, a heterotrophic sulfur-oxidizing acidophile, was isolated. Its genome was sequenced and then used for comparative genomics. Furthermore, real-time quantitative PCR was performed to identify the expression of genes involved in the RISC oxidation. Gene encoding thiosulfate: quinone oxidoreductase was present inAcidicaldussp. strain DX-1, while no candidate genes with significant similarity to tetrathionate hydrolase were found. Additionally, there were genes encoding heterodisulfide reductase complex, which was proposed to play a crucial role in oxidizing cytoplasmic sulfur. Like many heterotrophic sulfur oxidizers,Acidicaldussp. strain DX-1 had no genes encoding enzymes essential for the direct oxidation of sulfite. An indirect oxidation of sulfite via adenosine-5′-phosphosulfate was proposed inAcidicaldusstrain DX-1. However, compared to other closely related bacteriaAcidiphilium cryptumandAcidiphilium multivorum, which harbored the genes encoding Sox system, almost all of these genes were not detected inAcidicaldussp. strain DX-1. This study might provide some references for the future study of RISC oxidation in heterotrophic sulfur-oxidizing acidophiles.

Molecular characterization of inorganic sulfur-compound metabolism in the deep-sea epsilonproteobacterium Sulfurovum sp. NBC37-1

2010 ◽  
Vol 12 (5) ◽  
pp. 1144-1153 ◽  
Author(s):  
Masahiro Yamamoto ◽  
Satoshi Nakagawa ◽  
Shigeru Shimamura ◽  
Ken Takai ◽  
Koki Horikoshi
Keyword(s):  
Molecular Characterization ◽  
Deep Sea ◽  
Sulfur Compound ◽  
Inorganic Sulfur ◽  
Inorganic Sulfur Compound

Effects of Diphenylether Herbicides on Reactions of Mitochondria and Chloroplasts

Weed Science ◽  
1970 ◽  
Vol 18 (5) ◽  
pp. 636-642 ◽  
Author(s):  
D. E. Moreland ◽  
W. J. Blackmon ◽  
H. G. Todd ◽  
F. S. Farmer
Keyword(s):  
Electron Transport ◽  
Oxygen Uptake ◽  
Spinacia Oleracea ◽  
White Potato ◽  
Light Reaction ◽  
Site Of Action ◽  
Transport Inhibitors ◽  
Atp Generation ◽  
A Site ◽  
Limited Oxygen

Effects of three diphenylether herbicides [2,4-dichlorophenyl-p-nitrophenyl ether (nitrofen); 2,4,6-trichlorophenyl-4′-nitrophenyl ether (hereinafter referred to as MC-1478); and 2,4′-dinitro-4-trifluoromethyl-diphenylether (hereinafter referred to as C-6989)] were measured on phosphorylation and electron transport in spinach(Spinacia oleraceaL.) chloroplasts, and mung bean(Phaseolus aureusL., var. Jumbo) and white potato tuber(Solarium tuberosumL.) mitochondria. All of the diphenylethers acted primarily as inhibitors of chloroplast noncyclic electron transport, and the coupled photophosphorylation. The compounds ranked in the following decreasing order of inhibitory effectiveness: MC-1478 ≥ C-6989 >> nitrofen. A site of action close to light reaction II was suggested. At high molar concentrations, marginal interference with cyclic electron transport or phosphorylation was obtained. In mitochondria, MC-1478 and nitrofen acted primarily as electron transport inhibitors with malate, NADH, and succinate as substrates. MC-1478 was a slightly stronger inhibitor than nitrofen. Only slight stimulation of ADP-limited oxygen uptake was obtained during the oxidation of NADH and succinate; whereas, strong inhibition of oxygen uptake was obtained with malate. C-6989 also weakly stimulated ADP-limited oxygen uptake with NADH and succinate but differed from the two chlorinated diphenylethers in that electron transport was not inhibited when ADP was present in excess. Interference with ATP generation could be one of the mechanisms through which the phytotoxicity of diphenylether herbicides is expressed.

Sign in / Sign up

Export Citation Format

Share Document