Pcal_1699, an extremely thermostable malate dehydrogenase from hyperthermophilic archaeon Pyrobaculum calidifontis

Extremophiles ◽  
2015 ◽  
Vol 20 (1) ◽  
pp. 57-67 ◽  
Author(s):  
Ghazaleh Gharib ◽  
Naeem Rashid ◽  
Qamar Bashir ◽  
Qura-tul Ann Afza Gardner ◽  
Muhammad Akhtar ◽  
...  
Keyword(s):  
Malate Dehydrogenase ◽  
Hyperthermophilic Archaeon ◽  
Pyrobaculum Calidifontis

scholarly journals Unique Presence of a Manganese Catalase in a Hyperthermophilic Archaeon, Pyrobaculum calidifontis VA1

2002 ◽  
Vol 184 (12) ◽  
pp. 3305-3312 ◽  
Author(s):  
Taku Amo ◽  
Haruyuki Atomi ◽  
Tadayuki Imanaka
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Catalase Activity ◽  
Specific Activity ◽  
Homology Search ◽  
Aerobic Conditions ◽  
Hyperthermophilic Archaeon ◽  
Manganese Catalase ◽  
Catalase Gene ◽  
Pyrobaculum Calidifontis

ABSTRACT We had previously isolated a facultatively anaerobic hyperthermophilic archaeon, Pyrobaculum calidifontis strain VA1. Here, we found that strain VA1, when grown under aerobic conditions, harbors high catalase activity. The catalase was purified 91-fold from crude extracts and displayed a specific activity of 23,500 U/mg at 70°C. The enzyme exhibited a Km value of 170 mM toward H2O2 and a k cat value of 2.9 × 104 s−1·subunit−1 at 25°C. Gel filtration chromatography indicated that the enzyme was a homotetramer with a subunit molecular mass of 33,450 Da. The purified catalase did not display the Soret band, which is an absorption band particular to heme enzymes. In contrast to typical heme catalases, the catalase was not strongly inhibited by sodium azide. Furthermore, with plasma emission spectroscopy, we found that the catalase did not contain iron but instead contained manganese. Our biochemical results indicated that the purified catalase was not a heme catalase but a manganese (nonheme) catalase, the first example in archaea. Intracellular catalase activity decreased when cells were grown anaerobically, while under aerobic conditions, an increase in activity was observed with the removal of thiosulfate from the medium, or addition of manganese. Based on the N-terminal amino acid sequence of the purified protein, we cloned and sequenced the catalase gene (katPc ). The deduced amino acid sequence showed similarity with that of the manganese catalase from a thermophilic bacterium, Thermus sp. YS 8-13. Interestingly, in the complete archaeal genome sequences, no open reading frame has been assigned as a manganese catalase gene. Moreover, a homology search with the sequence of katPc revealed that no orthologue genes were present on the archaeal genomes, including those from the “aerobic” (hyper)thermophilic archaea Aeropyrum pernix, Sulfolobus solfataricus, and Sulfolobus tokodaii. Therefore, Kat Pc can be considered a rare example of a manganese catalase from archaea.

Crystal structure of UDP-galactose 4-epimerase from the hyperthermophilic archaeon Pyrobaculum calidifontis

2011 ◽  
Vol 512 (2) ◽  
pp. 126-134 ◽  
Author(s):  
Haruhiko Sakuraba ◽  
Tomoyuki Kawai ◽  
Kazunari Yoneda ◽  
Toshihisa Ohshima
Keyword(s):  
Crystal Structure ◽  
Hyperthermophilic Archaeon ◽  
Pyrobaculum Calidifontis

Characterization of a novel dye-linked l-proline dehydrogenase from an aerobic hyperthermophilic archaeon, Pyrobaculum calidifontis

2010 ◽  
Vol 89 (4) ◽  
pp. 1075-1082 ◽  
Author(s):  
Takenori Satomura ◽  
Xiao-Dong Zhang ◽  
Yusuke Hara ◽  
Katsumi Doi ◽  
Haruhiko Sakuraba ◽  
...  
Keyword(s):  
Proline Dehydrogenase ◽  
Hyperthermophilic Archaeon ◽  
Pyrobaculum Calidifontis
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