X-ray absorption spectroscopic studies of the methane monooxygenase hydroxylase component from Methylosinus trichosporium OB3b

1996 ◽  
Vol 1 (4) ◽  
pp. 297-304 ◽  
Author(s):  
Lijin Shu ◽  
Y. Liu ◽  
J. D. Lipscomb ◽  
L. Que
Keyword(s):  
Methane Monooxygenase ◽  
Spectroscopic Studies ◽  
Methylosinus Trichosporium Ob3b ◽  
Methylosinus Trichosporium ◽  
X Ray ◽  
X Ray Absorption

Spectroscopic studies of the methane monooxygenase from Methylosinus trichosporium OB3b

1989 ◽  
Vol 36 (3-4) ◽  
pp. 322 ◽  
Author(s):  
B.G. Fox ◽  
J.D. Lipscomb ◽  
K.K. Surerus ◽  
M.P. Hendrich ◽  
E. Münck
Keyword(s):  
Methane Monooxygenase ◽  
Spectroscopic Studies ◽  
Methylosinus Trichosporium Ob3b ◽  
Methylosinus Trichosporium

X-ray Absorption Spectroscopic Studies of the Diiron Center in Methane Monooxygenase in the Presence of Substrate and the Coupling Protein of the Enzyme System

1995 ◽  
Vol 34 (10) ◽  
pp. 2505-2515 ◽  
Author(s):  
Jane G. DeWitt ◽  
Amy C. Rosenzweig ◽  
Athanasios Salifoglou ◽  
Britt Hedman ◽  
Stephen J. Lippard ◽  
...  
Keyword(s):  
Enzyme System ◽  
Methane Monooxygenase ◽  
Spectroscopic Studies ◽  
X Ray ◽  
Coupling Protein ◽  
X Ray Absorption

scholarly journals Methanobactin from Methylocystis sp. Strain SB2 Affects Gene Expression and Methane Monooxygenase Activity in Methylosinus trichosporium OB3b

2015 ◽  
Vol 81 (7) ◽  
pp. 2466-2473 ◽  
Author(s):  
Muhammad Farhan Ul-Haque ◽  
Bhagyalakshmi Kalidass ◽  
Alexey Vorobev ◽  
Bipin S. Baral ◽  
Alan A. DiSpirito ◽  
...  
Keyword(s):  
Gene Expression ◽  
Methane Monooxygenase ◽  
Particulate Methane Monooxygenase ◽  
Methylosinus Trichosporium Ob3b ◽  
Methylosinus Trichosporium ◽  
Monooxygenase Activity ◽  
Content Type ◽  
Soluble Methane Monooxygenase ◽  
A Subunit ◽  
Membrane Bound

ABSTRACTMethanotrophs can express a cytoplasmic (soluble) methane monooxygenase (sMMO) or membrane-bound (particulate) methane monooxygenase (pMMO). Expression of these MMOs is strongly regulated by the availability of copper. Many methanotrophs have been found to synthesize a novel compound, methanobactin (Mb), that is responsible for the uptake of copper, and methanobactin produced byMethylosinus trichosporiumOB3b plays a key role in controlling expression of MMO genes in this strain. As all known forms of methanobactin are structurally similar, it was hypothesized that methanobactin from one methanotroph may alter gene expression in another. WhenMethylosinus trichosporiumOB3b was grown in the presence of 1 μM CuCl2, expression ofmmoX, encoding a subunit of the hydroxylase component of sMMO, was very low.mmoXexpression increased, however, when methanobactin fromMethylocystissp. strain SB2 (SB2-Mb) was added, as did whole-cell sMMO activity, but there was no significant change in the amount of copper associated withM. trichosporiumOB3b. IfM. trichosporiumOB3b was grown in the absence of CuCl2, themmoXexpression level was high but decreased by several orders of magnitude if copper prebound to SB2-Mb (Cu-SB2-Mb) was added, and biomass-associated copper was increased. Exposure ofMethylosinus trichosporiumOB3b to SB2-Mb had no effect on expression ofmbnA, encoding the polypeptide precursor of methanobactin in either the presence or absence of CuCl2.mbnAexpression, however, was reduced when Cu-SB2-Mb was added in both the absence and presence of CuCl2. These data suggest that methanobactin acts as a general signaling molecule in methanotrophs and that methanobactin “piracy” may be commonplace.

scholarly journals Towards a unified understanding of the copper sites in particulate methane monooxygenase: An X-ray absorption spectroscopic investigation

2021 ◽  
Author(s):  
George Cutsail III ◽  
Matthew O Ross ◽  
Amy C Rosenzweig ◽  
Serena DeBeer
Keyword(s):  
Greenhouse Gas ◽  
Spectroscopic Investigation ◽  
Liquid Fuel ◽  
Methane Monooxygenase ◽  
Enzymatic Conversion ◽  
Particulate Methane Monooxygenase ◽  
X Ray ◽  
Mild Conditions ◽  
Copper Sites ◽  
X Ray Absorption

The enzymatic conversion of the greenhouse gas, methane, to a liquid fuel, methanol, is performed by methane monooxygenases (MMOs) under mild conditions. The copper stoichiometry of particulate MMO (pMMO) has...

X-ray Absorption Fine Structure Spectroscopic Studies of Octakis(DMSO)lanthanoid(III) Complexes in Solution and in the Solid Iodides

2007 ◽  
Vol 46 (19) ◽  
pp. 7742-7748 ◽  
Author(s):  
Ingmar Persson ◽  
Emiliana Damian Risberg ◽  
Paola D'Angelo ◽  
Simone De Panfilis ◽  
Magnus Sandström ◽  
...  
Keyword(s):  
Fine Structure ◽  
Spectroscopic Studies ◽  
X Ray ◽  
Absorption Fine ◽  
X Ray Absorption

scholarly journals High-Energy-Resolution Fluorescence-Detected X-ray Absorption of the Q Intermediate of Soluble Methane Monooxygenase

2017 ◽  
Vol 139 (49) ◽  
pp. 18024-18033 ◽  
Author(s):  
Rebeca G. Castillo ◽  
Rahul Banerjee ◽  
Caleb J. Allpress ◽  
Gregory T. Rohde ◽  
Eckhard Bill ◽  
...  
Keyword(s):  
Energy Resolution ◽  
Methane Monooxygenase ◽  
High Energy ◽  
High Energy Resolution ◽  
X Ray ◽  
Soluble Methane Monooxygenase ◽  
X Ray Absorption
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