Is there a rate-limiting step in the catalytic cycle of [Ni-Fe] hydrogenases?

2000 ◽  
Vol 5 (6) ◽  
pp. 682-691 ◽  
Author(s):  
Patrick Bertrand ◽  
François Dole ◽  
Marcel Asso ◽  
Bruno Guigliarelli
Keyword(s):  
Catalytic Cycle ◽  
Rate Limiting Step ◽  
Limiting Step ◽  
Rate Limiting

Is the Internal Electron Transfer the Rate-Limiting Step in the Catalytic Cycle of Cytochrome c Oxidase?

1988 ◽  
Vol 550 (1 Cytochrome Ox) ◽  
pp. 161-166 ◽  
Author(s):  
PAOLO SARTI ◽  
GIOVANNI ANTONINI ◽  
RANCESCO MALATESTA ◽  
BEATRICE VALLONE ◽  
MAURIZIO BRUNORI
Keyword(s):  
Electron Transfer ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Catalytic Cycle ◽  
Rate Limiting Step ◽  
Limiting Step ◽  
Internal Electron ◽  
Rate Limiting

scholarly journals How VASP enhances actin-based motility

2003 ◽  
Vol 163 (1) ◽  
pp. 131-142 ◽  
Author(s):  
Stanislav Samarin ◽  
Stéphane Romero ◽  
Christine Kocks ◽  
Dominique Didry ◽  
Dominique Pantaloni ◽  
...  
Keyword(s):  
Actin Filaments ◽  
Actin Polymerization ◽  
Living Cells ◽  
Catalytic Cycle ◽  
Motility Assay ◽  
Regulatory Proteins ◽  
Capping Proteins ◽  
Rate Limiting Step ◽  
Limiting Step ◽  
Rate Limiting

The function of vasodilator-stimulated phosphoprotein (VASP) in motility is analyzed using a biomimetic motility assay in which ActA-coated microspheres propel themselves in a medium containing actin, the Arp2/3 complex, and three regulatory proteins in the absence or presence of VASP. Propulsion is linked to cycles of filament barbed end attachment-branching-detachment-growth in which the ActA-activated Arp2/3 complex incorporates at the junctions of branched filaments. VASP increases the velocity of beads. VASP increases branch spacing of filaments in the actin tail, as it does in lamellipodia in living cells. The effect of VASP on branch spacing of Arp2/3-induced branched actin arrays is opposed to the effect of capping proteins. However, VASP does not compete with capping proteins for binding barbed ends of actin filaments. VASP enhances branched actin polymerization only when ActA is immobilized on beads or on Listeria. VASP increases the rate of dissociation of the branch junction from immobilized ActA, which is the rate-limiting step in the catalytic cycle of site-directed filament branching.

scholarly journals Evidence That the Pi Release Event Is the Rate-Limiting Step in the Nitrogenase Catalytic Cycle

Biochemistry ◽  
2016 ◽  
Vol 55 (26) ◽  
pp. 3625-3635 ◽  
Author(s):  
Zhi-Yong Yang ◽  
Rhesa Ledbetter ◽  
Sudipta Shaw ◽  
Natasha Pence ◽  
Monika Tokmina-Lukaszewska ◽  
...  
Keyword(s):  
Catalytic Cycle ◽  
Rate Limiting Step ◽  
Limiting Step ◽  
Rate Limiting

Ornithine Decarboxylase Activity in Cultured Endothelial Cells Stimulated by Serum, Thrombin and Serotonin

1978 ◽  
Vol 39 (02) ◽  
pp. 496-503 ◽  
Author(s):  
P A D’Amore ◽  
H B Hechtman ◽  
D Shepro
Keyword(s):  
Endothelial Cells ◽  
Ornithine Decarboxylase ◽  
Decarboxylase Activity ◽  
Aortic Endothelial Cells ◽  
Ornithine Decarboxylase Activity ◽  
Rate Limiting Step ◽  
Bovine Aortic Endothelial Cells ◽  
Limiting Step ◽  
Rate Limiting ◽  
Serum Serotonin

SummaryOrnithine decarboxylase (ODC) activity, the rate-limiting step in the synthesis of polyamines, can be demonstrated in cultured, bovine, aortic endothelial cells (EC). Serum, serotonin and thrombin produce a rise in ODC activity. The serotonin-induced ODC activity is significantly blocked by imipramine (10-5 M) or Lilly 11 0140 (10-6M). Preincubation of EC with these blockers together almost completely depresses the 5-HT-stimulated ODC activity. These observations suggest a manner by which platelets may maintain EC structural and metabolic soundness.

Dynamics of hepatic and peripheral insulin effects suggest common rate-limiting step in vivo

Diabetes ◽  
1993 ◽  
Vol 42 (2) ◽  
pp. 296-306 ◽  
Author(s):  
D. C. Bradley ◽  
R. A. Poulin ◽  
R. N. Bergman
Keyword(s):  
Rate Limiting Step ◽  
Limiting Step ◽  
Rate Limiting
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