Comparison of the S RNA segments among Japanese isolates and Taiwanese isolates of Watermelon silver mottle virus

2001 ◽  
Vol 146 (2) ◽  
pp. 389-394 ◽  
Author(s):  
M. Okuda ◽  
S. Taba ◽  
S. Tsuda ◽  
S. Hidaka ◽  
M. Kameya-Iwaki ◽  
...  
Keyword(s):  
Mottle Virus ◽  
Watermelon Silver Mottle Virus

scholarly journals Comparison of Ambisense M RNA of Watermelon Silver Mottle Virus with Other Tospoviruses

1998 ◽  
Vol 88 (4) ◽  
pp. 351-358 ◽  
Author(s):  
Fang-Hua Chu ◽  
Shyi-Dong Yeh
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Consensus Sequence ◽  
Chenopodium Quinoa ◽  
Distinct Species ◽  
Mottle Virus ◽  
Impatiens Necrotic Spot Virus ◽  
Cdna Clones ◽  
Genomic Rnas ◽  
Watermelon Silver Mottle Virus

Double-stranded genomic RNAs (dsRNAs) extracted from Chenopodium quinoa infected with watermelon silver mottle virus (WSMV) were similar to those of tomato spotted wilt virus (TSWV, serogroup I) and impatiens necrotic spot virus (INSV, serogroup III), except that the S dsRNA of WSMV is 0.75 and 0.6 kbp longer than those of TSWV and INSV, respectively. The complete nucleotide sequence of the genomic M RNA of WSMV was determined from cDNA clones generated from separated M dsRNA. The M RNA is 4,880 nucleotides in length with two open reading frames (ORFs) in an ambisense organization. The M RNA-encoded nonstructural (NSm) ORF located on the viral strand encodes a protein of 312 amino acids (35 kDa), and the G1/G2 ORF located on the viral complementary strand encodes a protein of 1,121 amino acids (127.6 kDa). The RNA probe corresponding to the NSm or G1/G2 ORF of WSMV failed to hybridize with the M dsRNAs of TSWV and INSV. Comparison of M and S RNAs of WSMV, TSWV, INSV, and peanut bud necrosis virus (PBNV, serogroup IV) revealed a consensus sequence of eight nucleotides of 5′-AGAGCAAU…-3′ at their 5′ ends and 5′-…AUUGCUCU-3′ at their 3′ ends. The low overall nucleotide identities (56.4 to 56.9%) of the M RNA and the low amino acid identities of the NSm and G1/G2 proteins (30.5 to 40.9%) with those of TSWV and INSV indicate that WSMV belongs to the Tospovirus genus but is phylogenetically distinct from viruses in serogroups I and III. The M RNA of WSMV shares a nucleotide identity of 79.6% with that of PBNV, and the two viruses share 83.4 and 88.7% amino acid identities for their NSm and G1/G2 proteins, respectively. It is concluded that they are two related but distinct species of serogroup IV. In addition to the viral or viral complementary full-length M RNA, two putative RNA messages for the NSm gene and the G1/G2 gene, 1.0 and 3.4 kb, respectively, were detected from the total RNA extracted from WSMV-infected tissue of Nicotiana benthamiana. The 1.0- and 3.4-kb RNAs were also detected in the viral RNAs extracted from purified nucleocapsids, suggesting that the putative messages of the M RNA of WSMV can also be encapsidated by the nucleocapsid protein.

Watermelon silver mottle orthotospovirus. [Distribution map].

2018 ◽  
Author(s):  
Keyword(s):  
Solanum Lycopersicum ◽  
Geographical Distribution ◽  
Cucumis Melo ◽  
Citrullus Lanatus ◽  
Mottle Virus ◽  
Ryukyu Archipelago ◽  
Distribution Map ◽  
Watermelon Silver Mottle Virus ◽  
New Distribution

Abstract A new distribution map is provided for Watermelon silver mottle virus. Bunyavirales: Bunyaviridae: Orthotospovirus. Hosts: Cucurbitaceae especially watermelon (Citrullus lanatus), melon (Cucumis melo), tomato (Solanum lycopersicum), Capsicum. Information is given on the geographical distribution in Asia (China, Guangdong, Yunnan, India, Maharashtra, Japan, Kyushu, Ryukyu Archipelago, Taiwan, Thailand).

scholarly journals A Chlorotic Spot Disease on Calla Lilies (Zantedeschia spp.) Is Caused by a Tospovirus Serologically but Distantly Related to Watermelon silver mottle virus

Plant Disease ◽  
2005 ◽  
Vol 89 (5) ◽  
pp. 440-445 ◽  
Author(s):  
C. C. Chen ◽  
T. C. Chen ◽  
Y. H. Lin ◽  
S. D. Yeh ◽  
H. T. Hsu
Keyword(s):  
Mottle Virus ◽  
N Gene ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
Chlorotic Spot ◽  
Calla Lily ◽  
Thrips Palmi ◽  
Benincasa Hispida ◽  
Watermelon Silver Mottle Virus ◽  
Wax Gourd

A new tospovirus, Calla lily chlorotic spot virus (CCSV), was isolated from calla lilies (Zantedeschia spp.) in Taiwan. Chlorotic spots, ranging from light green to yellow, appear on the middle leaves of the affected plants. Virions measuring 75 to 105 nm, similar in size to tospovirus particles, were present in crude extracts and ultrathin sections of diseased leaves. Of 35 plant species inoculated mechanically, 24, including wax gourd (Benincasa hispida) and zucchini squash (Cucurbita pepo), were susceptible to the virus. CCSV was transmitted from infected wax gourd by Thrips palmi to healthy wax gourd and zucchini squash. The virus was weakly related to Watermelon silver mottle virus (WSMoV) in enzyme-linked immunosorbent assay (ELISA) and western blot tests. WSMoV-specific N gene primers, however, failed to produce DNA fragments from total RNA extracts of CCSV-infected plants in reverse transcription-polymerase chain reaction (RT-PCR). Results of RT-PCR show that the conserved regions of the L genes of tospoviruses are present in CCSV.

scholarly journals Two Novel Motifs of Watermelon Silver Mottle Virus NSs Protein Are Responsible for RNA Silencing Suppression and Pathogenicity

PLoS ONE ◽  
2015 ◽  
Vol 10 (5) ◽  
pp. e0126161 ◽  
Author(s):  
Chung-Hao Huang ◽  
Weng-Rong Hsiao ◽  
Ching-Wen Huang ◽  
Kuan-Chun Chen ◽  
Shih-Shun Lin ◽  
...  
Keyword(s):  
Rna Silencing ◽  
Mottle Virus ◽  
Silencing Suppression ◽  
Watermelon Silver Mottle Virus

scholarly journals A Conserved Helix in the C-Terminal Region of Watermelon Silver Mottle Virus Nonstructural Protein S Is Imperative For Protein Stability Affecting Self-Interaction, RNA Silencing Suppression, and Pathogenicity

2020 ◽  
Vol 33 (4) ◽  
pp. 637-652
Author(s):  
Chung-Hao Huang ◽  
Mung-Hsia Foo ◽  
Joseph A. J. Raja ◽  
Yue-Rong Tan ◽  
Tzu-Tung Lin ◽  
...  
Keyword(s):  
Amino Acids ◽  
Rna Silencing ◽  
Nonstructural Protein ◽  
Protein S ◽  
Binding Activity ◽  
Terminal Region ◽  
Bimolecular Fluorescence Complementation ◽  
Mottle Virus ◽  
Α Helix ◽  
Watermelon Silver Mottle Virus

In orthotospovirus, the nonstructural protein S (NSs) is the RNA-silencing suppressor (RSS) and pathogenicity determinant. Here, we demonstrate that a putative α-helix, designated H8, spanning amino acids 338 to 369 of the C-terminal region of the NSs protein, is crucial for self-interaction of watermelon silver mottle virus NSs protein and that the H8 affects RSS function. Co-immunoprecipitation, yeast two-hybrid, and bimolecular fluorescence complementation analyses revealed that the triple point mutation (TPM) of H8 amino acids Y338A, H350A, and F353A resulted in NSs protein self-interaction dysfunction. Transient expression of H8-deleted (ΔH8) and TPM NSs proteins in Nicotiana benthamiana plants by agroinfitration indicated that these proteins have weaker RSS activity and are far less stable than wild-type (WT) NSs. However, an electrophoretic mobility assay revealed that small interfering RNA (siRNA) binding ability of TPM NSs protein is not compromised. The pathogenicity assay of WT NSs protein expressed by the attenuated turnip mosaic virus vector restored severe symptoms in recombinant-infected N. benthamiana plants but not for ΔH8 or TPM proteins. Taken together, we conclude that the H8 helix in the C-terminal region of NSs protein is crucial for stabilizing NSs protein through self-interaction to maintain normal functions of RSS and pathogenicity, but not for NSs-siRNA binding activity.

Serological Comparison and Molecular Characterization for Verification of Calla lily chlorotic spot virus as a New Tospovirus Species Belonging to Watermelon silver mottle virus Serogroup

2005 ◽  
Vol 95 (12) ◽  
pp. 1482-1488 ◽  
Author(s):  
Yu-Hsuan Lin ◽  
Tsung-Chi Chen ◽  
Hei-Ti Hsu ◽  
Fang-Lin Liu ◽  
Fang-Hua Chu ◽  
...  
Keyword(s):  
Open Reading Frames ◽  
Mottle Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Spot Virus ◽  
Heterologous Antigen ◽  
Chlorotic Spot ◽  
Calla Lily ◽  
Homologous Antigen ◽  
Distinct Member ◽  
Watermelon Silver Mottle Virus

Calla lily chlorotic spot virus (CCSV) isolated from central Taiwan was recently identified as a tospovirus serologically but distantly related to Watermelon silver mottle virus (WSMoV). To clarify the serological relationship between the two viruses, rabbit polyclonal antibody (PAb) to CCSV and mouse monoclonal antibodies (MAbs) to WSMoV NP or CCSV NP were produced in this investigation, using purified nucleocapsid protein (NP) as immunogens. The PAb to CCSV NP reacted stronger with the homologous antigen than with the heterologous antigen, with much lower A405 readings in indirect enzyme-linked immunosorbent assay (ELISA) and low-intensity banding in immunoblotting. MAbs produced to CCSV NP or WSMoV NP reacted specifically with the homologous antigens but not with the heterologous antigens in both ELISA and immunoblot analyses. The CCSV S RNA was determined to be 3,172 nucleotides in length, with an inverted repeat at the 5′ and 3′ ends and two open reading frames encoding the NP and a nonstructural (NSs) protein in an ambisense arrangement. A typical 3′-terminal sequence (5′-AUUGCUCU-3′) that is shared by all members of the genus Tospovirus also is present in the CCSV S RNA. The CCSV NP and NSs protein share low amino acid identities of 20.1 to 65.1% and 19.9 to 66.1%, respectively, with those of reported tospoviruses. Phylogenetic dendrogram analysis indicates that CCSV is a distinct member in the genus Tospovirus. The results provide evidence that CCSV is a new species in the genus Tospovirus and belongs to WSMoV serogroup.

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