Vibriophage KVP40 and coliphage T4 genomes share a homologous 7-kbp region immediately upstream of the gene encoding the major capsid protein

1999 ◽  
Vol 144 (10) ◽  
pp. 2007-2012 ◽  
Author(s):  
S. Matsuzaki ◽  
M. Kuroda ◽  
S. Kimura ◽  
S. Tanaka
Keyword(s):  
Capsid Protein ◽  
Major Capsid Protein ◽  
Gene Encoding

scholarly journals Inducible Gene Expression from African Swine Fever Virus Recombinants: Analysis of the Major Capsid Protein p72

1998 ◽  
Vol 72 (4) ◽  
pp. 3185-3195 ◽  
Author(s):  
Ramón García-Escudero ◽  
Germán Andrés ◽  
Fernando Almazán ◽  
Eladio Viñuela
Keyword(s):  
Capsid Protein ◽  
Major Capsid Protein ◽  
African Swine Fever Virus ◽  
Expression System ◽  
African Swine Fever ◽  
Firefly Luciferase ◽  
Fever Virus ◽  
Gene Encoding ◽  
Iptg Induction ◽  
Dense Membrane

ABSTRACT A method to study the function of individual African swine fever virus (ASFV) gene products utilizing the Escherichia coli lac repressor-operator system has been developed. Recombinant viruses containing both the lacI gene encoding thelac repressor and a strong virus late promoter modified by the insertion of one or two copies of the lac operator sequence at various positions were constructed. The ability of each modified promoter to regulate expression of the firefly luciferase gene was assayed in the presence and in the absence of the inducer isopropyl β-d-thiogalactoside (IPTG). Induction and repression of gene activity were dependent on the position(s) of the operator(s) with respect to the promoter and on the number of operators inserted. The ability of this system to regulate the expression of ASFV genes was analyzed by constructing a recombinant virus inducibly expressing the major capsid protein p72. Electron microscopy analysis revealed that under nonpermissive conditions, electron-dense membrane-like structures accumulated in the viral factories and capsid formation was inhibited. Induction of p72 expression allowed the progressive building of the capsid on these structures, leading to assembly of ASFV particles. The results of this report demonstrate that the transferred inducible expression system is a powerful tool for analyzing the function of ASFV genes.

Sequence variation in the gene encoding the major capsid protein of Korean fish iridoviruses

2004 ◽  
Vol 150 (2) ◽  
pp. 351-359 ◽  
Author(s):  
J. W. Do ◽  
S. J. Cha ◽  
J. S. Kim ◽  
E. J. An ◽  
M. S. Park ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Sequence Variation ◽  
Major Capsid Protein ◽  
Gene Encoding

scholarly journals Identification of a nonconventional motif necessary for the nuclear import of the human parvovirus B19 major capsid protein (VP2)

Virology ◽  
2003 ◽  
Vol 306 (1) ◽  
pp. 25-32 ◽  
Author(s):  
Sylvie Pillet ◽  
Zeinab Annan ◽  
Serge Fichelson ◽  
F.rédéric Morinet
Keyword(s):  
Capsid Protein ◽  
Nuclear Import ◽  
Major Capsid Protein ◽  
Parvovirus B19 ◽  
Human Parvovirus B19
Sign in / Sign up

Export Citation Format

Share Document