scholarly journals Transmissible gastroenteritis virus induced apoptosis in swine testes cell cultures

1998 ◽  
Vol 143 (12) ◽  
pp. 2471-2485 ◽  
Author(s):  
T. Sirinarumitr ◽  
J. P. Kluge ◽  
P. S. Paul
Keyword(s):  
Cell Cultures ◽  
Transmissible Gastroenteritis Virus ◽  
Gastroenteritis Virus ◽  
Transmissible Gastroenteritis ◽  
Induced Apoptosis

scholarly journals miR-27b attenuates apoptosis induced by transmissible gastroenteritis virus (TGEV) infection via targeting runt-related transcription factor 1 (RUNX1)

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e1635 ◽  
Author(s):  
Xiaomin Zhao ◽  
Xiangjun Song ◽  
Xiaoyuan Bai ◽  
Naijiao Fei ◽  
Yong Huang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Caspase 3 ◽  
Transmissible Gastroenteritis Virus ◽  
Over Expression ◽  
Gastroenteritis Virus ◽  
Related Transcription Factor ◽  
Infected Cells ◽  
Transmissible Gastroenteritis ◽  
Induced Apoptosis ◽  
Transcription Factor 1

Transmissible gastroenteritis virus (TGEV), belonging to the coronaviridae family, is the key cause of the fatal diarrhea of piglets and results in many pathological processes. microRNAs (miRNAs) play a key role in the regulation of virus-induced apoptosis. During the process of apoptosis induced by TGEV infection in PK-15 cells, the miR-27b is notably down-regulated. Thus, we speculate that miR-27b is involved in regulating the process of apoptosis in PK-15 cells. In this study we demonstrated that the over-expression of miR-27b led to the inhibition of TGEV-induced apoptosis, reduction of Bax protein level, and decrease of caspase-3 and −9 activities. Conversely, silencing of miR-27b by miR-27b inhibitors enhanced apoptosis via up-regulating Bax expression and promoting the activities of caspase-3 and −9 in TGEV-infected cells. Subsequently, the runt-related transcription factor 1 (RUNX1) is a candidate target of miR-27b predicted by bioinformatics search. We further identified that the miR-27b directly bound to the 3′ UTR of RUNX1 mRNA and suppressed RUNX1 expression, which indicates RUNX1 is the direct target gene of miR-27b. The over-expression of RUNX1 increased apoptosis and knockdown RUNX1blocked apoptosis of viral-infected cells via regulating Bax expression and the activities of caspase-3 and −9. Our data reveal that miR-27b may repress the mitochondrial pathway of apoptosis by targeting RUNX1, indicating that TGEV may induce apoptosis via down-regulating miR-27b and that miR-27b may act as a target for therapeutic intervention.

scholarly journals Reverse Genetic Analysis of the Transcription Regulatory Sequence of the Coronavirus Transmissible Gastroenteritis Virus

2004 ◽  
Vol 78 (11) ◽  
pp. 6061-6066 ◽  
Author(s):  
Kristopher M. Curtis ◽  
Boyd Yount ◽  
Amy C. Sims ◽  
Ralph S. Baric
Keyword(s):  
Genetic Analysis ◽  
Full Length ◽  
Transmissible Gastroenteritis Virus ◽  
Regulatory Sequence ◽  
Reverse Genetic ◽  
Conserved Sequence ◽  
Gastroenteritis Virus ◽  
Flanking Sequences ◽  
Transmissible Gastroenteritis ◽  
Discontinuous Transcription

ABSTRACT Coronavirus discontinuous transcription uses a highly conserved sequence (CS) in the joining of leader and body RNAs. Using a full-length infectious construct of transmissable gastroenteritis virus, the present study demonstrates that subgenomic transcription is heavily influenced by upstream flanking sequences and supports a mechanism of transcription attenuation that is regulated in part by a larger domain composed of primarily upstream flanking sequences which select appropriately positioned CS elements for synthesis of subgenomic RNAs.

scholarly journals The Application of Immunogold Silver Staining (IGSS) for the Detection of Transmissible Gastroenteritis Virus in Fixed Tissues

1993 ◽  
Vol 5 (1) ◽  
pp. 16-20 ◽  
Author(s):  
Renke Larochelle ◽  
Ronald Magar
Keyword(s):  
Silver Staining ◽  
Protein A ◽  
Immunogold Electron Microscopy ◽  
Transmissible Gastroenteritis Virus ◽  
Tissue Sections ◽  
Gastroenteritis Virus ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Transmissible Gastroenteritis ◽  
Immune Aggregates

Protein A-gold (PAG) and a primary porcine antiserum were used in immunogold silver staining (IGSS) for the detection of transmissible gastroenteritis virus (TGEV) in formalin-fixed paraffin-embedded tissue sections of small intestine originating from infected pigs. Immunogold electron microscopy was used to evaluate the reactivity of the prepared PAG marker with the specific porcine TGEV antiserum. Gold particles were closely associated with single virions and immune aggregates of TGEV. When IGSS, using PAG as the marker, was applied to tissue sections, dark staining of TGEV-infected villous enterocytes was observed. Background was low, allowing good visualization by light microscopy of the distribution of viral antigen. Two other gold conjugates, protein A/G-gold (PA/GG) and protein G-gold (PGG), were tested in IGSS. The labeling with PA/GG was comparable to that obtained with PAG. However, no staining was observed when PGG was used. The use of IGSS and PAG offers advantages and may represent a useful technique for the detection of other viral pathogens.

Sign in / Sign up

Export Citation Format

Share Document